##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631815.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9058
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50275999116803	34.0	31.0	34.0	31.0	34.0
2	32.70423934643409	34.0	31.0	34.0	31.0	34.0
3	32.78008390373151	34.0	31.0	34.0	31.0	34.0
4	36.258335173327445	37.0	37.0	37.0	35.0	37.0
5	36.15378670788253	37.0	35.0	37.0	35.0	37.0
6	36.18580260543166	37.0	36.0	37.0	35.0	37.0
7	36.17939942592184	37.0	36.0	37.0	35.0	37.0
8	36.13005078383749	37.0	36.0	37.0	35.0	37.0
9	37.96919849856481	39.0	38.0	39.0	35.0	39.0
10	37.84080370942813	39.0	38.0	39.0	35.0	39.0
11	37.953632148377125	39.0	38.0	39.0	35.0	39.0
12	37.880547582247736	39.0	38.0	39.0	35.0	39.0
13	37.9571649370722	39.0	38.0	39.0	35.0	39.0
14	39.333517332744535	40.0	39.0	41.0	36.0	41.0
15	39.32413336277324	40.0	39.0	41.0	36.0	41.0
16	39.296975049679844	40.0	39.0	41.0	36.0	41.0
17	39.27853830867741	40.0	39.0	41.0	36.0	41.0
18	39.22024729520866	40.0	39.0	41.0	36.0	41.0
19	39.264959152130714	40.0	39.0	41.0	36.0	41.0
20	39.257120777213515	40.0	39.0	41.0	36.0	41.0
21	39.19253698388165	40.0	39.0	41.0	36.0	41.0
22	39.16880105983661	40.0	39.0	41.0	36.0	41.0
23	39.1097372488408	40.0	39.0	41.0	36.0	41.0
24	39.13877235592846	40.0	39.0	41.0	36.0	41.0
25	39.12287480680062	40.0	39.0	41.0	35.0	41.0
26	39.058070214175316	40.0	39.0	41.0	35.0	41.0
27	38.933760211967325	40.0	38.0	41.0	35.0	41.0
28	38.931221020092735	40.0	38.0	41.0	35.0	41.0
29	38.87017001545595	40.0	38.0	41.0	35.0	41.0
30	38.84775888717156	40.0	38.0	41.0	35.0	41.0
31	38.72035769485537	40.0	38.0	41.0	35.0	41.0
32	38.70015455950541	40.0	38.0	41.0	35.0	41.0
33	38.67984102450872	40.0	38.0	41.0	35.0	41.0
34	38.59969088098918	40.0	38.0	41.0	35.0	41.0
35	38.44071538971075	40.0	38.0	41.0	34.0	41.0
36	38.46268491940826	40.0	38.0	41.0	34.0	41.0
37	38.42548023846324	40.0	38.0	41.0	34.0	41.0
38	38.36807242216825	40.0	38.0	41.0	34.0	41.0
39	38.28770147935526	40.0	38.0	41.0	34.0	41.0
40	38.01115036431884	40.0	37.0	41.0	33.0	41.0
41	38.05575182159417	40.0	37.0	41.0	33.0	41.0
42	38.025171119452416	40.0	37.0	41.0	33.0	41.0
43	37.519319938176196	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	3.0
24	7.0
25	7.0
26	14.0
27	28.0
28	44.0
29	53.0
30	67.0
31	104.0
32	144.0
33	185.0
34	257.0
35	358.0
36	547.0
37	978.0
38	2046.0
39	4214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27666151468315	15.842349304482225	12.188120998012806	28.69286818282181
2	21.174652241112828	17.608743652020316	32.87701479355266	28.339589313314196
3	21.627290792669463	19.319938176197837	28.869507617575625	30.183263413557075
4	15.952748951203354	14.429233826451757	33.51733274453522	36.10068447780967
5	17.100905277103113	32.77765511150365	32.788695076175756	17.332744535217486
6	35.537646279531906	33.20821373371605	14.683153013910355	16.570986972841688
7	31.717818502980787	27.01479355266063	19.308898211525722	21.958489732832856
8	27.224552881430782	31.03334069330978	18.999779200706556	22.74232722455288
9	28.1739898432325	12.938838595716492	16.924265842349303	41.9629057187017
10	18.889379553985428	23.78008390373151	29.598145285935086	27.73239125634798
11	39.35747405608302	18.436741002428793	18.845219695296976	23.36056524619121
12	25.436078604548467	23.23912563479797	24.60808125413999	26.71671450651358
13	33.826451755354384	18.02826230956061	20.35769485537646	27.787591079708545
14	24.497681607418855	19.15433870611614	22.896886729962464	33.451092956502535
15	29.785824685361007	23.371605210863326	19.882976374475604	26.959593729300067
16	26.694634577169353	23.415765069551778	20.699933760211966	29.189666593066903
17	26.738794435857805	23.857363656436302	21.064252594391697	28.339589313314196
18	26.50695517774343	20.78825347758887	23.294325458158536	29.41046588650916
19	28.75910797085449	21.627290792669463	22.80856701258556	26.805034223890484
20	29.741664826672555	20.85449326562155	22.885846765290353	26.517995142415547
21	29.344226098476483	21.07529255906381	21.859130050783836	27.721351291675866
22	29.63126517995142	22.30072863766836	20.368734820048576	27.69927136233164
23	28.49414881872378	22.135129167586665	20.89865312431	28.472068889379553
24	28.77014793552661	20.99801280635902	21.32921174652241	28.90262751159196
25	28.483108854051668	20.622654007507176	23.040406270699933	27.853830867741223
26	28.284389489953632	22.19032899094723	22.642967542503865	26.882313976595274
27	28.16294987856039	21.163612276440716	22.344888496356813	28.328549348642085
28	26.484875248399202	21.605210863325237	23.20600574078163	28.703908147493927
29	28.30646941929786	21.527931110620447	22.8416869066019	27.323912563479798
30	27.44535217487304	20.08169573857364	25.071759770368736	27.401192316184584
31	27.853830867741223	21.39545153455509	22.720247295208658	28.03047030249503
32	25.855597262088757	22.400088319717376	22.952086553323028	28.792227864870835
33	27.467432104217266	21.638330757341574	24.177522631927577	26.71671450651358
34	28.372709207330537	21.848090086111725	23.06248620004416	26.71671450651358
35	26.992713623316405	22.687127401192317	23.813203797747846	26.50695517774343
36	27.147273128725992	22.090969308898213	23.007286376683595	27.754471185692204
37	25.89975712077721	21.892249944800177	24.100242879222787	28.107750055199826
38	27.058953411349083	20.99801280635902	25.458158533892693	26.484875248399202
39	25.745197615367633	20.86553323029366	25.281519099138883	28.107750055199826
40	25.08279973504085	20.655773901523517	27.43431221020093	26.82711415323471
41	24.497681607418855	19.463457716935306	27.345992492824024	28.69286818282181
42	22.786487083241333	21.274011923161844	27.599911680282624	28.339589313314196
43	22.433208213733717	19.353058070214175	29.36630602782071	28.8474276882314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	0.5
9	0.0
10	2.0
11	4.0
12	4.0
13	3.0
14	2.0
15	9.5
16	17.0
17	15.5
18	14.0
19	14.0
20	16.0
21	18.0
22	17.0
23	16.0
24	15.0
25	14.0
26	14.0
27	16.5
28	19.0
29	30.5
30	42.0
31	52.0
32	62.0
33	62.0
34	75.5
35	89.0
36	104.0
37	119.0
38	184.0
39	249.0
40	249.0
41	288.5
42	328.0
43	384.5
44	441.0
45	528.0
46	615.0
47	615.0
48	634.0
49	653.0
50	766.5
51	880.0
52	902.0
53	924.0
54	924.0
55	929.0
56	934.0
57	855.5
58	777.0
59	713.0
60	649.0
61	649.0
62	636.0
63	623.0
64	500.5
65	378.0
66	339.5
67	301.0
68	301.0
69	244.5
70	188.0
71	196.0
72	204.0
73	210.0
74	216.0
75	216.0
76	190.5
77	165.0
78	114.5
79	64.0
80	44.5
81	25.0
82	25.0
83	17.5
84	10.0
85	9.0
86	8.0
87	7.0
88	6.0
89	6.0
90	4.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9058.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.77655111503643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49467707594039	68.05034223890483
2	6.784953867991483	10.554206226540074
3	2.256919801277502	5.266063148597924
4	1.249112845990064	3.8860675645837937
5	0.9226401703335699	3.587988518436741
6	0.397444996451384	1.8547140649149922
7	0.24130589070262598	1.313755795981453
8	0.227111426543648	1.4131154780304702
9	0.07097232079488999	0.49679841024508725
>10	0.35486160397444994	3.5769485537646277
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	25	0.27599911680282624	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	18	0.1987193640980349	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	17	0.18767939942592185	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	16	0.17663943475380878	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	15	0.16559947008169573	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	15	0.16559947008169573	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	15	0.16559947008169573	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	14	0.1545595054095827	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	14	0.1545595054095827	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	13	0.14351954073746964	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	13	0.14351954073746964	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	12	0.1324795760653566	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	12	0.1324795760653566	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	12	0.1324795760653566	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	11	0.12143961139324355	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	11	0.12143961139324355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.12143961139324355	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	10	0.11039964672113049	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	10	0.11039964672113049	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	10	0.11039964672113049	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	10	0.11039964672113049	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	10	0.11039964672113049	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	10	0.11039964672113049	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	10	0.11039964672113049	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	10	0.11039964672113049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.011039964672113049	0.0
23	0.0	0.0	0.0	0.011039964672113049	0.0
24	0.0	0.0	0.0	0.011039964672113049	0.0
25	0.0	0.0	0.0	0.011039964672113049	0.0
26	0.0	0.0	0.0	0.011039964672113049	0.0
27	0.0	0.0	0.0	0.044159858688452194	0.0
28	0.0	0.0	0.0	0.12143961139324354	0.0
29	0.0	0.0	0.0	0.27599911680282624	0.0
30	0.0	0.0	0.0	0.4967984102450872	0.0
31	0.0	0.0	0.0	0.90527710311327	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGGGAA	25	0.00521661	29.6	18
>>END_MODULE