FastQCFastQC Report
Fri 10 Feb 2017
ERR1631814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723026
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41270.2395204715425072No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA39420.22878354708518617No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA38430.22303784156478193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36410.21131428080597736No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA36400.2112562433764784No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA34300.19906838318168152No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC33430.1940191268152657No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG32040.1859519241149002No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA31590.1833402397874437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30960.17968388172900468No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT30020.17422836335609562No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC29300.17004966843216526No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27300.15844218253235876No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA26930.15629479764089457No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT25430.14758918321603967No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA22890.1328476761232854No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA18880.10957466689417339No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA18570.10777550657970338No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18060.10481559767525273No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17720.10284232507228562No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT17710.10278428764278658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.029.487311
GTTATCG909.48512E-820.55555511
GTATCAA43800.019.809362
TCTATCG759.270174E-619.73333431
CTTATAC16800.019.6011937
AGTATTA951.6773265E-719.47368415
TCTATAC951.6773265E-719.4736843
TAATACT1700.018.54
GACCGTT1900.018.57
TTAGACG609.2393643E-418.54
CGTTACG1002.8769864E-718.516
CAGTCGG7200.018.510
TATACCC1002.8769864E-718.55
TATAACA500.00703627118.54
TATACAC5150.018.32038937
ATACGGC2350.018.10638429
CTAGTTC2050.018.0487823
GCCTAGT1750.017.971431
AGTCGGT7550.017.88741511
GCAGTCG7350.017.8707499
Produced by FastQC (version 0.11.5)