##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631814.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1723026
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67709134975328	34.0	31.0	34.0	31.0	34.0
2	32.835602596826746	34.0	31.0	34.0	31.0	34.0
3	32.921746392683566	34.0	31.0	34.0	31.0	34.0
4	36.33323583045178	37.0	37.0	37.0	35.0	37.0
5	36.25484061180737	37.0	37.0	37.0	35.0	37.0
6	36.31083686491092	37.0	37.0	37.0	35.0	37.0
7	36.298761016955055	37.0	37.0	37.0	35.0	37.0
8	36.282669559252156	37.0	37.0	37.0	35.0	37.0
9	38.12118621541404	39.0	39.0	39.0	37.0	39.0
10	38.057812244272576	39.0	38.0	39.0	35.0	39.0
11	38.130922574586805	39.0	38.0	39.0	37.0	39.0
12	38.06895949335645	39.0	38.0	39.0	37.0	39.0
13	38.12079852538499	39.0	38.0	39.0	37.0	39.0
14	39.52057310800882	41.0	39.0	41.0	37.0	41.0
15	39.53003669126293	41.0	39.0	41.0	37.0	41.0
16	39.47900554025302	41.0	39.0	41.0	37.0	41.0
17	39.448773262852676	41.0	39.0	41.0	37.0	41.0
18	39.480085616816	41.0	39.0	41.0	37.0	41.0
19	39.50091931288326	41.0	39.0	41.0	37.0	41.0
20	39.500825872621775	41.0	39.0	41.0	37.0	41.0
21	39.46581363252789	41.0	39.0	41.0	37.0	41.0
22	39.44522775628458	41.0	39.0	41.0	37.0	41.0
23	39.38217473212824	41.0	39.0	41.0	37.0	41.0
24	39.3938808816582	41.0	39.0	41.0	36.0	41.0
25	39.34435812344097	41.0	39.0	41.0	36.0	41.0
26	39.28765439407182	40.0	39.0	41.0	36.0	41.0
27	39.1767187494559	40.0	39.0	41.0	36.0	41.0
28	39.17079951202129	40.0	39.0	41.0	36.0	41.0
29	39.142592160536175	40.0	39.0	41.0	35.0	41.0
30	39.103046036449825	40.0	39.0	41.0	35.0	41.0
31	39.01200736378905	40.0	39.0	41.0	35.0	41.0
32	38.9643412229415	40.0	38.0	41.0	35.0	41.0
33	38.9050374167308	40.0	38.0	41.0	35.0	41.0
34	38.87494500953555	40.0	38.0	41.0	35.0	41.0
35	38.801015771091095	40.0	38.0	41.0	35.0	41.0
36	38.755408798242165	40.0	38.0	41.0	35.0	41.0
37	38.70696495583932	40.0	38.0	41.0	35.0	41.0
38	38.67064455208453	40.0	38.0	41.0	35.0	41.0
39	38.594917894448486	40.0	38.0	41.0	35.0	41.0
40	38.27237197813614	40.0	38.0	41.0	34.0	41.0
41	38.34025661829827	40.0	38.0	41.0	34.0	41.0
42	38.32134976489037	40.0	38.0	41.0	34.0	41.0
43	37.85885064996117	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	1.0
14	2.0
15	2.0
16	3.0
17	4.0
18	15.0
19	30.0
20	55.0
21	121.0
22	207.0
23	412.0
24	741.0
25	1251.0
26	2000.0
27	3186.0
28	4857.0
29	7369.0
30	10433.0
31	14443.0
32	19786.0
33	27522.0
34	40278.0
35	61007.0
36	94693.0
37	175459.0
38	400584.0
39	858562.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.64398796071562	16.67856433971397	13.063819118225725	27.613628581344678
2	20.01502008675435	19.93939731611711	33.6832990332125	26.36228356391604
3	20.710540641870757	20.41571050001567	30.072036057494202	28.801712800619377
4	14.918289103008311	16.521050756053594	35.121466536198525	33.43919360473957
5	16.17445122708537	33.357012604568936	34.162862313163004	16.305673855182683
6	32.50931790930607	34.707601626440926	16.272070183502745	16.51101028075026
7	29.485799982124473	28.574960563566655	20.735090474548844	21.204148979760028
8	26.893848380697676	31.225936230793966	20.671539489247408	21.20867589926095
9	28.259527134239416	13.268226944921318	18.49316261043072	39.97908331040855
10	18.848932053259787	24.011825706634724	30.37255386744019	26.7666883726653
11	37.71916384314572	20.45122940686908	19.581538525826076	22.248068224159127
12	22.192642478987548	24.741704420014557	27.465226874115658	25.600426226882238
13	34.119682465615725	18.46785829116914	21.789282343969273	25.623176899245863
14	23.527097095458803	21.017152381914144	24.31391052717719	31.141839995449867
15	28.82510188470749	25.009721269441087	20.996433019582987	25.168743826268436
16	24.43863296317061	25.072749917877037	23.49181033832339	26.99680678062896
17	25.7228852031252	24.642054153564715	22.965701039914663	26.66935960339542
18	25.76066756972907	22.218469135114617	24.630736854812405	27.39012644034391
19	27.09291676387936	23.150376140580583	24.7570263014023	24.999680794137756
20	28.907805221743605	21.628402589397954	24.103060545807203	25.36073164305124
21	26.393913962993015	23.189377293203933	23.31061748342741	27.106091260375642
22	27.315432268578654	23.72697800265347	22.78462425987768	26.172965468890197
23	26.660189689534576	23.007081727147472	23.82662826910331	26.506100314214642
24	26.72263796367553	22.566577637249814	24.34008540788125	26.370698991193404
25	26.56634316603464	22.95444177859185	24.382975068281034	26.096239987092474
26	26.50023853383524	23.6533865420487	23.752920733639538	26.09345419047652
27	26.060430893091574	22.61486477859301	25.194512444966005	26.130191883349408
28	25.373093615534533	23.665516364813996	23.580955830033908	27.38043418961757
29	26.104887564087832	24.113681395405525	23.791515624256395	25.98991541625025
30	25.83187949572438	23.033720907287528	24.98981443112292	26.14458516586517
31	26.659609315239585	23.37445865587635	24.167540129980626	25.79839189890344
32	24.51657723098781	23.315956926941322	24.345308776536164	27.822157065534704
33	24.75354405563236	22.80801334396579	25.981151764395893	26.45729083600596
34	25.298921780634764	22.80017829098342	25.257425018542957	26.643474909838854
35	24.76828556272511	24.28077115493324	25.708259770891445	25.242683511450203
36	25.1408278226794	23.156818295254975	25.942556873779036	25.759797008286583
37	25.593519772771856	22.94068690780058	25.493463244315524	25.972330075112044
38	24.92370979892352	22.267336650752803	26.341796351302882	26.46715719902079
39	24.522671161085206	21.46787105940363	27.321410123817053	26.68804765569411
40	24.118614576912943	22.31603005410249	27.952335019901035	25.61302034908353
41	22.536398173910317	21.588240688184623	28.97373574165451	26.90162539625055
42	21.408266619308126	21.97012697428826	29.544882085354484	27.076724321049134
43	20.68512024775018	22.80017829098342	29.33902332292142	27.175678138344985
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	19.0
2	30.0
3	87.5
4	145.0
5	145.0
6	209.5
7	274.0
8	295.5
9	317.0
10	539.0
11	761.0
12	761.0
13	1543.5
14	2326.0
15	4771.5
16	7217.0
17	7030.0
18	6843.0
19	6843.0
20	7689.0
21	8535.0
22	7551.0
23	6567.0
24	7284.5
25	8002.0
26	8002.0
27	9120.5
28	10239.0
29	12979.0
30	15719.0
31	18570.0
32	21421.0
33	21421.0
34	25721.5
35	30022.0
36	33292.0
37	36562.0
38	43857.0
39	51152.0
40	51152.0
41	59335.0
42	67518.0
43	76004.5
44	84491.0
45	110712.5
46	136934.0
47	136934.0
48	150014.5
49	163095.0
50	182097.5
51	201100.0
52	200396.0
53	199692.0
54	199692.0
55	177621.5
56	155551.0
57	144208.0
58	132865.0
59	120774.0
60	108683.0
61	108683.0
62	98294.5
63	87906.0
64	72283.0
65	56660.0
66	48715.0
67	40770.0
68	40770.0
69	34598.0
70	28426.0
71	24307.5
72	20189.0
73	16348.0
74	12507.0
75	12507.0
76	10243.5
77	7980.0
78	6528.5
79	5077.0
80	4080.5
81	3084.0
82	3084.0
83	2533.5
84	1983.0
85	1577.0
86	1171.0
87	926.5
88	682.0
89	682.0
90	525.0
91	368.0
92	246.0
93	124.0
94	74.5
95	25.0
96	25.0
97	15.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1723026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.73927325016207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50439027329386	37.83639163502263
2	6.753245664141846	5.907641148587449
3	2.021696657363501	2.6528262757608436
4	1.042833168592413	1.8245105966158355
5	0.6326387435212266	1.3835579435757066
6	0.43385736357151117	1.1385963466109545
7	0.3067900073571789	0.9393140373550414
8	0.23533669286565506	0.8234764732032283
9	0.19004429852867105	0.7481159552682826
>10	1.455553245110491	13.07123655681879
>50	0.22054448576606067	6.80404725162585
>100	0.179233771144301	15.312099700896855
>500	0.014913912956750371	4.515419694528846
>1k	0.008921715786627453	7.04276638412976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4127	0.2395204715425072	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3942	0.22878354708518617	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3843	0.22303784156478193	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3641	0.21131428080597736	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3640	0.2112562433764784	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	3430	0.19906838318168152	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	3343	0.1940191268152657	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	3204	0.1859519241149002	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3159	0.1833402397874437	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3096	0.17968388172900468	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	3002	0.17422836335609562	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2930	0.17004966843216526	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2730	0.15844218253235876	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	2693	0.15629479764089457	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2543	0.14758918321603967	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2289	0.1328476761232854	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1888	0.10957466689417339	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1857	0.10777550657970338	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1806	0.10481559767525273	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1772	0.10284232507228562	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1771	0.10278428764278658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.803742949903252E-5	0.0
5	5.803742949903252E-5	0.0	0.0	5.803742949903252E-5	0.0
6	1.1607485899806503E-4	0.0	0.0	5.803742949903252E-5	0.0
7	1.1607485899806503E-4	0.0	0.0	5.803742949903252E-5	0.0
8	3.482245769941951E-4	0.0	0.0	5.803742949903252E-5	0.0
9	3.482245769941951E-4	0.0	0.0	5.803742949903252E-5	0.0
10	4.062620064932276E-4	0.0	0.0	5.803742949903252E-5	5.803742949903252E-5
11	4.062620064932276E-4	0.0	0.0	1.1607485899806503E-4	5.803742949903252E-5
12	4.6429943599226014E-4	0.0	0.0	1.7411228849709755E-4	5.803742949903252E-5
13	4.6429943599226014E-4	0.0	0.0	1.7411228849709755E-4	5.803742949903252E-5
14	4.6429943599226014E-4	0.0	0.0	2.3214971799613007E-4	5.803742949903252E-5
15	5.803742949903252E-4	0.0	0.0	2.901871474951626E-4	5.803742949903252E-5
16	6.384117244893577E-4	0.0	0.0	6.964491539883902E-4	5.803742949903252E-5
17	8.125240129864552E-4	0.0	0.0	0.0011607485899806503	5.803742949903252E-5
18	8.705614424854878E-4	0.0	0.0	0.0012187860194796828	5.803742949903252E-5
19	8.705614424854878E-4	0.0	0.0	0.0018571977439690406	5.803742949903252E-5
20	9.285988719845203E-4	0.0	0.0	0.0025536468979574308	5.803742949903252E-5
21	0.0010446737309825853	0.0	0.0	0.004526919500924536	5.803742949903252E-5
22	0.0011027111604816177	0.0	0.0	0.007486828405375194	5.803742949903252E-5
23	0.0011027111604816177	0.0	0.0	0.008821689283852942	5.803742949903252E-5
24	0.0011027111604816177	0.0	0.0	0.01062084959832295	5.803742949903252E-5
25	0.0011607485899806503	0.0	0.0	0.012652159630789089	5.803742949903252E-5
26	0.0011607485899806503	0.0	0.0	0.01590225568273491	5.803742949903252E-5
27	0.0012768234489787154	0.0	0.0	0.035170682276413706	5.803742949903252E-5
28	0.0013348608784777478	0.0	0.0	0.1226330885314557	5.803742949903252E-5
29	0.0013348608784777478	0.0	0.0	0.26935171030500993	5.803742949903252E-5
30	0.0013348608784777478	0.0	0.0	0.4521115757974633	5.803742949903252E-5
31	0.0013348608784777478	0.0	0.0	0.9715465698138043	5.803742949903252E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2955	0.0	29.48731	1
GTTATCG	90	9.48512E-8	20.555555	11
GTATCAA	4380	0.0	19.80936	2
TCTATCG	75	9.270174E-6	19.733334	31
CTTATAC	1680	0.0	19.60119	37
AGTATTA	95	1.6773265E-7	19.473684	15
TCTATAC	95	1.6773265E-7	19.473684	3
TAATACT	170	0.0	18.5	4
GACCGTT	190	0.0	18.5	7
TTAGACG	60	9.2393643E-4	18.5	4
CGTTACG	100	2.8769864E-7	18.5	16
CAGTCGG	720	0.0	18.5	10
TATACCC	100	2.8769864E-7	18.5	5
TATAACA	50	0.007036271	18.5	4
TATACAC	515	0.0	18.320389	37
ATACGGC	235	0.0	18.106384	29
CTAGTTC	205	0.0	18.048782	3
GCCTAGT	175	0.0	17.97143	1
AGTCGGT	755	0.0	17.887415	11
GCAGTCG	735	0.0	17.870749	9
>>END_MODULE