FastQCFastQC Report
Fri 10 Feb 2017
ERR1631813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104071
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68750.32674752895696013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58060.27594125863623425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54050.2568829664017992No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA39270.1866381885402156No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA29750.1413925670759209No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA27040.12851277357085383No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA25200.11976782152313302No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG23930.11373190353367355No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC23730.11278136526761692No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC22090.10498695148595272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.031.467291
TATCCGG1153.0540832E-919.30434810
TTTTACG1750.019.0285724
GCGGTAA12100.018.65289323
ATATACG500.00703677818.4999986
CTTATAC29000.018.3086237
GTATCAA46100.018.1789592
CGCGGTA12650.017.98814222
GTACTGG15550.017.964631
TTCTGCG10400.017.78846218
TTACTCG953.6092679E-617.52631629
TACTGGT15550.017.2508052
CTATCCG1401.87174E-917.1785729
TGCGGGT10500.017.09047521
CCGCGGT13500.016.99259421
CAGTCGG3950.016.8607610
GGTAATA13400.016.84328325
GTGTAAT1107.815179E-716.8181821
GCGGGTA10750.016.69302422
GTAACGT10550.016.48341226
Produced by FastQC (version 0.11.5)