FastQCFastQC Report
Fri 10 Feb 2017
ERR1631812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86323
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2560.29656059219443254No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2100.24327236078449543No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1960.2270542033988624No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1850.21431136545300788No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1790.2073607265734509No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1500.17376597198892532No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1430.1656568932961088No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1360.15754781460329229No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1350.1563893747900328No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1310.1517556155369948No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1270.14712185628395677No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1180.13669589796462123No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1150.13322057852484273No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1110.12858681927180474No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1090.1262699396452857No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1080.1251114998320262No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1020.11816086095246922No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1010.11700242113920972No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA950.1100517822596527No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT930.10773490263313369No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA910.1054180230066147No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG910.1054180230066147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCC250.005468567429.59999835
TATGCCC250.005468567429.59999836
GATATGC250.005468567429.59999834
TGTAACT250.005468567429.59999836
GCGGTAA851.8189894E-1228.29411723
CGGTAAT905.456968E-1226.72222124
ATTGATA358.8028173E-426.42857231
AGCCGCG959.094947E-1225.3157919
GGTAATA959.094947E-1225.3157925
TCCGTGC959.094947E-1225.315798
AACTCCG959.094947E-1225.315795
CCGTGCC1051.8189894E-1224.6666669
TAATACG1002.0008883E-1124.0527
CGCGGTA1002.0008883E-1124.0522
CGTGCCA1002.0008883E-1124.0510
ATACGGA1002.0008883E-1124.0529
AATACGG1002.0008883E-1124.0528
GTGCCAG1002.0008883E-1124.0511
CTTATTG400.001916828823.12528
CTTATAC802.6526322E-823.12537
Produced by FastQC (version 0.11.5)