##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631812.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86323
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.667771045955305	34.0	31.0	34.0	31.0	34.0
2	32.826465715973725	34.0	31.0	34.0	31.0	34.0
3	32.90879603350208	34.0	31.0	34.0	31.0	34.0
4	36.326876962107434	37.0	37.0	37.0	35.0	37.0
5	36.244731994949206	37.0	35.0	37.0	35.0	37.0
6	36.294822932474545	37.0	37.0	37.0	35.0	37.0
7	36.27895230703289	37.0	37.0	37.0	35.0	37.0
8	36.26386942066425	37.0	37.0	37.0	35.0	37.0
9	38.09758696986898	39.0	38.0	39.0	37.0	39.0
10	38.03462576601833	39.0	38.0	39.0	35.0	39.0
11	38.12592240770131	39.0	38.0	39.0	37.0	39.0
12	38.04732226637165	39.0	38.0	39.0	35.0	39.0
13	38.12054724696778	39.0	38.0	39.0	37.0	39.0
14	39.507454560198326	41.0	39.0	41.0	37.0	41.0
15	39.522896562909075	41.0	39.0	41.0	37.0	41.0
16	39.457409960265515	41.0	39.0	41.0	37.0	41.0
17	39.42841421173963	40.0	39.0	41.0	37.0	41.0
18	39.439720584317044	41.0	39.0	41.0	37.0	41.0
19	39.46354969127579	41.0	39.0	41.0	37.0	41.0
20	39.4738598056138	41.0	39.0	41.0	37.0	41.0
21	39.43683606918203	41.0	39.0	41.0	37.0	41.0
22	39.40254625070954	40.0	39.0	41.0	37.0	41.0
23	39.33480069043013	40.0	39.0	41.0	36.0	41.0
24	39.357228085214835	41.0	39.0	41.0	36.0	41.0
25	39.30995215643571	40.0	39.0	41.0	36.0	41.0
26	39.24929624781345	40.0	39.0	41.0	36.0	41.0
27	39.13959199749777	40.0	39.0	41.0	36.0	41.0
28	39.13413574597732	40.0	39.0	41.0	36.0	41.0
29	39.10716726712464	40.0	39.0	41.0	35.0	41.0
30	39.08566662419054	40.0	39.0	41.0	35.0	41.0
31	39.0067884573057	40.0	39.0	41.0	35.0	41.0
32	38.96064779954357	40.0	38.0	41.0	35.0	41.0
33	38.897292726156415	40.0	38.0	41.0	35.0	41.0
34	38.86754399175191	40.0	38.0	41.0	35.0	41.0
35	38.82626878120547	40.0	38.0	41.0	35.0	41.0
36	38.768914426051	40.0	38.0	41.0	35.0	41.0
37	38.72728009916245	40.0	38.0	41.0	35.0	41.0
38	38.69539983550155	40.0	38.0	41.0	35.0	41.0
39	38.59193957577934	40.0	38.0	41.0	35.0	41.0
40	38.307913302364376	40.0	38.0	41.0	34.0	41.0
41	38.39128621572466	40.0	38.0	41.0	34.0	41.0
42	38.39574620900571	40.0	38.0	41.0	34.0	41.0
43	37.91419436303187	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	1.0
21	8.0
22	14.0
23	19.0
24	39.0
25	60.0
26	117.0
27	151.0
28	215.0
29	347.0
30	481.0
31	724.0
32	987.0
33	1397.0
34	2123.0
35	3181.0
36	4844.0
37	8760.0
38	20288.0
39	42565.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.6213176094436	16.507767338947904	12.915445478030188	27.955469573578306
2	20.75113237491746	19.89272847329217	32.071406230089316	27.284732921701053
3	20.98513721719588	19.670308029146348	29.284200039386953	30.060354714270822
4	15.513825979171253	15.869467001841919	34.24000556051111	34.37670145847572
5	16.79737729226278	33.11284362220961	33.62487401967031	16.4649050658573
6	34.10099278291997	33.88668141746696	15.454745548695017	16.557580250918065
7	30.28161671860338	27.566233796323115	19.96455174171426	22.187597743359245
8	27.439963856677824	30.678961574551394	20.403600430939612	21.47747413783117
9	28.370191026725205	12.496090265630249	17.621027999490284	41.51269070815426
10	19.096880321582894	23.631013750680584	29.57149311307531	27.700612814661213
11	39.25489151211149	19.66219895045353	18.495650058501212	22.58725947893377
12	22.974178376562445	23.790878444910398	26.96036977398839	26.274573404538764
13	35.54209191061478	17.415984152543356	20.672358467615815	26.369565469226046
14	24.100181875050684	20.141793033142964	23.017040649653048	32.74098444215331
15	29.628256663925022	24.253095930400935	19.942541385262327	26.17610602041171
16	25.742849530252656	24.14999478702084	22.436662303210035	27.67049337951647
17	27.233761569917636	23.8766029910916	21.52497017017481	27.36466526881596
18	26.4796172514857	21.625754433928385	23.368047913070676	28.52658040151524
19	28.070155115090994	22.20265746093162	23.261471450250802	26.465715973726585
20	30.31405303337465	20.741864856411386	22.532812807710574	26.41126930250339
21	27.55001563893748	22.184122423919465	21.858600836393546	28.40726110074951
22	28.654008780973783	22.369472794040984	21.31181724453506	27.66470118045017
23	28.197583494549537	21.995296734358167	22.258262571968075	27.54885719912422
24	27.819932115426944	21.53771300812066	22.67066714548846	27.97168773096394
25	28.146612142766124	22.213083419250953	22.532812807710574	27.10749163027235
26	27.768960763643523	22.73090601577795	22.469098617981302	27.03103460259722
27	27.18974085701377	21.653556989446614	23.852275755013146	27.304426398526466
28	26.181898219478008	22.880344751688426	22.369472794040984	28.56828423479258
29	27.137611065417094	23.61479559329495	22.157478308214497	27.09011503307346
30	26.45992377466029	22.35209619684209	23.91251462530264	27.275465403194975
31	27.789812680282193	22.875710992435387	22.783035807374628	26.551440519907786
32	25.540122562932243	22.36020527553491	22.932474543285103	29.16719761824774
33	26.13556062694763	21.910730627990223	24.438446300522457	27.515262444539694
34	26.210859214809496	21.785619128158196	24.138410388888246	27.865111268144066
35	25.719680733987467	23.6877773015303	24.400217786684895	26.192324177797342
36	26.004656928049304	22.159795187841016	25.156678984743348	26.678868899366332
37	26.766910325174052	21.900304669670888	24.461615096787646	26.87116990836741
38	25.930516780000694	21.3106588047218	25.55170696106484	27.207117454212664
39	25.064003799682588	20.208982542312015	26.894338704632602	27.8326749533728
40	24.838108036096983	21.442720943433383	27.553490958377257	26.165680062092374
41	22.974178376562445	20.583158601994832	28.609988068069924	27.8326749533728
42	21.758975012453227	20.98397877738262	29.614355386165915	27.64269082399824
43	20.98513721719588	21.432294985114048	29.70703057122667	27.875537226463397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	3.0
7	3.0
8	8.0
9	13.0
10	15.0
11	17.0
12	17.0
13	34.0
14	51.0
15	97.5
16	144.0
17	131.0
18	118.0
19	118.0
20	156.5
21	195.0
22	166.0
23	137.0
24	143.5
25	150.0
26	150.0
27	194.5
28	239.0
29	337.5
30	436.0
31	563.0
32	690.0
33	690.0
34	863.5
35	1037.0
36	1170.5
37	1304.0
38	1622.0
39	1940.0
40	1940.0
41	2375.0
42	2810.0
43	3211.0
44	3612.0
45	4934.5
46	6257.0
47	6257.0
48	7039.0
49	7821.0
50	8813.0
51	9805.0
52	10155.0
53	10505.0
54	10505.0
55	9556.5
56	8608.0
57	8086.0
58	7564.0
59	7071.5
60	6579.0
61	6579.0
62	5996.5
63	5414.0
64	4494.5
65	3575.0
66	3081.5
67	2588.0
68	2588.0
69	2139.5
70	1691.0
71	1463.0
72	1235.0
73	967.5
74	700.0
75	700.0
76	569.5
77	439.0
78	358.5
79	278.0
80	218.5
81	159.0
82	159.0
83	126.5
84	94.0
85	77.5
86	61.0
87	44.5
88	28.0
89	28.0
90	23.0
91	18.0
92	10.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	86323.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.504512123072644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.37846367135482	55.489267055130156
2	5.574709498531531	7.080384138642076
3	2.196318794578522	4.184284605493321
4	1.2039621299184589	3.0582811070050857
5	0.7771028293110053	2.46747680224274
6	0.5454313285539686	2.078241024987547
7	0.3903755997008337	1.7353428402627342
8	0.2991663474342837	1.5198730349964666
9	0.22984731571170577	1.3136707482362753
>10	1.2860504569583537	14.28703821692944
>50	0.08573669713055693	3.760295633840344
>100	0.03283533081595797	3.0258447922338196
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	256	0.29656059219443254	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	210	0.24327236078449543	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	196	0.2270542033988624	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	185	0.21431136545300788	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	179	0.2073607265734509	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	150	0.17376597198892532	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	143	0.1656568932961088	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	136	0.15754781460329229	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	135	0.1563893747900328	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	131	0.1517556155369948	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	127	0.14712185628395677	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	118	0.13669589796462123	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	115	0.13322057852484273	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	111	0.12858681927180474	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	109	0.1262699396452857	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	108	0.1251114998320262	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	102	0.11816086095246922	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	101	0.11700242113920972	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	95	0.1100517822596527	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	93	0.10773490263313369	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	91	0.1054180230066147	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	91	0.1054180230066147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.001158439813259502	0.0
10	0.0	0.0	0.0	0.001158439813259502	0.0
11	0.0	0.0	0.0	0.001158439813259502	0.0
12	0.0	0.0	0.0	0.001158439813259502	0.0
13	0.0	0.0	0.0	0.001158439813259502	0.0
14	0.0	0.0	0.0	0.001158439813259502	0.0
15	0.0	0.0	0.0	0.001158439813259502	0.0
16	0.0	0.0	0.0	0.002316879626519004	0.0
17	0.0	0.0	0.0	0.002316879626519004	0.0
18	0.0	0.0	0.0	0.002316879626519004	0.0
19	0.0	0.0	0.0	0.002316879626519004	0.0
20	0.0	0.0	0.0	0.008109078692816515	0.0
21	0.0	0.0	0.0	0.011584398132595021	0.0
22	0.0	0.0	0.0	0.015059717572373527	0.0
23	0.0	0.0	0.0	0.018535037012152034	0.0
24	0.0	0.0	0.0	0.02201035645193054	0.0
25	0.0	0.0	0.0	0.023168796265190042	0.0
26	0.0	0.0	0.0	0.030119435144747055	0.0
27	0.0	0.0	0.0	0.05097135178341809	0.0
28	0.0	0.0	0.0	0.12974525908506424	0.0
29	0.0	0.0	0.0	0.31972938845962257	0.0
30	0.0	0.0	0.0	0.5294069946595925	0.0
31	0.0	0.0	0.0	1.0819827855843749	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCC	25	0.0054685674	29.599998	35
TATGCCC	25	0.0054685674	29.599998	36
GATATGC	25	0.0054685674	29.599998	34
TGTAACT	25	0.0054685674	29.599998	36
GCGGTAA	85	1.8189894E-12	28.294117	23
CGGTAAT	90	5.456968E-12	26.722221	24
ATTGATA	35	8.8028173E-4	26.428572	31
AGCCGCG	95	9.094947E-12	25.31579	19
GGTAATA	95	9.094947E-12	25.31579	25
TCCGTGC	95	9.094947E-12	25.31579	8
AACTCCG	95	9.094947E-12	25.31579	5
CCGTGCC	105	1.8189894E-12	24.666666	9
TAATACG	100	2.0008883E-11	24.05	27
CGCGGTA	100	2.0008883E-11	24.05	22
CGTGCCA	100	2.0008883E-11	24.05	10
ATACGGA	100	2.0008883E-11	24.05	29
AATACGG	100	2.0008883E-11	24.05	28
GTGCCAG	100	2.0008883E-11	24.05	11
CTTATTG	40	0.0019168288	23.125	28
CTTATAC	80	2.6526322E-8	23.125	37
>>END_MODULE