FastQCFastQC Report
Fri 10 Feb 2017
ERR1631811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887206
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16800.18935850298577783No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16640.18755508867162757No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16030.18067957159892967No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14470.1630962820359646No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14160.15960216680229847No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13680.15419192385984765No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13220.14900710770666564No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12450.1403281763198175No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11710.13198738511687252No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11300.12736613593686247No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11150.1256754350173466No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10610.11958891170708945No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10320.11632022326269209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATTC250.005495675429.625
GGTATCA11300.028.814161
GTCTACG451.3228801E-424.6666661
TGTATAA1201.0913936E-1121.58333410
TACGCAT450.00382513120.55555512
AGACCGT450.00382513120.5555556
GTATCAA15900.020.2452852
TATAAGA1202.382876E-1020.04166812
TTATTAC1052.2577296E-819.3809531
TCTTATA14250.018.69473637
GCGGTAA6800.018.523
AGACCGG1900.018.521
ATAAGAG1306.9485395E-1018.513
ACTAAAC609.2345517E-418.535
CGTGTAG500.007033647518.58
GTATAAG1409.458745E-1118.511
CTTATAC7050.018.36879337
TAATACG6400.018.21093827
CGCGGTA7150.018.11188722
GTAACGT4100.018.04878226
Produced by FastQC (version 0.11.5)