##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631811.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887206
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.639887466946796	34.0	31.0	34.0	31.0	34.0
2	32.80747875916078	34.0	31.0	34.0	31.0	34.0
3	32.90161135068969	34.0	31.0	34.0	31.0	34.0
4	36.31175172395137	37.0	37.0	37.0	35.0	37.0
5	36.22649756651781	37.0	35.0	37.0	35.0	37.0
6	36.27617035953319	37.0	37.0	37.0	35.0	37.0
7	36.26503202187541	37.0	37.0	37.0	35.0	37.0
8	36.24656167789668	37.0	37.0	37.0	35.0	37.0
9	38.083286181563246	39.0	38.0	39.0	37.0	39.0
10	38.00767690930855	39.0	38.0	39.0	35.0	39.0
11	38.08696289249622	39.0	38.0	39.0	37.0	39.0
12	38.02511254432454	39.0	38.0	39.0	35.0	39.0
13	38.07924991490139	39.0	38.0	39.0	37.0	39.0
14	39.48591195280465	41.0	39.0	41.0	37.0	41.0
15	39.48454135792589	41.0	39.0	41.0	37.0	41.0
16	39.43869518465836	40.0	39.0	41.0	37.0	41.0
17	39.39707576368961	40.0	39.0	41.0	37.0	41.0
18	39.40889489025097	41.0	39.0	41.0	37.0	41.0
19	39.422550118010925	41.0	39.0	41.0	37.0	41.0
20	39.41346767267129	40.0	39.0	41.0	37.0	41.0
21	39.3833901033131	40.0	39.0	41.0	37.0	41.0
22	39.35464480627949	40.0	39.0	41.0	36.0	41.0
23	39.287990613228494	40.0	39.0	41.0	36.0	41.0
24	39.30643728739436	40.0	39.0	41.0	36.0	41.0
25	39.23984846811226	40.0	39.0	41.0	36.0	41.0
26	39.182877482794304	40.0	39.0	41.0	36.0	41.0
27	39.07852629490783	40.0	39.0	41.0	35.0	41.0
28	39.06520920733178	40.0	39.0	41.0	35.0	41.0
29	39.045418989501876	40.0	39.0	41.0	35.0	41.0
30	39.00295647234126	40.0	39.0	41.0	35.0	41.0
31	38.917263859802574	40.0	38.0	41.0	35.0	41.0
32	38.87312078592796	40.0	38.0	41.0	35.0	41.0
33	38.8100937099163	40.0	38.0	41.0	35.0	41.0
34	38.774691559795585	40.0	38.0	41.0	35.0	41.0
35	38.70622606249282	40.0	38.0	41.0	35.0	41.0
36	38.66010374140842	40.0	38.0	41.0	35.0	41.0
37	38.60953262263781	40.0	38.0	41.0	35.0	41.0
38	38.57263702003819	40.0	38.0	41.0	35.0	41.0
39	38.49808499942516	40.0	38.0	41.0	34.0	41.0
40	38.171681661305264	40.0	37.0	41.0	34.0	41.0
41	38.24615929107783	40.0	37.0	41.0	34.0	41.0
42	38.22973920374749	40.0	37.0	41.0	34.0	41.0
43	37.75505463218238	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	4.0
18	3.0
19	19.0
20	32.0
21	47.0
22	89.0
23	221.0
24	434.0
25	671.0
26	1123.0
27	1789.0
28	2664.0
29	3956.0
30	5712.0
31	7999.0
32	10900.0
33	14842.0
34	22046.0
35	33772.0
36	52993.0
37	92877.0
38	211155.0
39	423851.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.14815274017534	16.066505411370077	12.438035811299743	28.347306037154844
2	20.891653122273745	18.93776642628657	32.39563303223829	27.7749474192014
3	21.206799773671502	19.43088752781203	28.73244770662056	30.629864991895907
4	15.754402021627445	15.371176479870515	33.33126692109837	35.54315457740367
5	17.44792077600918	32.292725702937084	33.00405993647473	17.255293584579004
6	35.33576193127639	32.93372677822287	15.093788815675277	16.636722474825465
7	31.21146610820937	27.3518213357439	19.802165449737714	21.634547106309018
8	28.182068200620826	30.345939950811875	19.703428516037988	21.76856333252931
9	28.65298476340331	12.673944946269526	17.570552949371397	41.102517340955764
10	19.440806306539855	23.308115589840465	29.72026789719637	27.53081020642331
11	39.49297006557666	19.118107857701595	18.794282274916988	22.594639801804767
12	23.596549166709874	23.3898328009504	26.261431956050796	26.752186076288936
13	34.642687267669515	17.50055793130344	20.629932619932685	27.226822181094356
14	24.20959732012633	19.468984655198454	23.01956929957642	33.30184872509879
15	29.598312004201954	23.791656052822006	20.00403513952791	26.60599680344813
16	26.25185131750687	23.74533084762727	21.87552834403735	28.12728949082851
17	27.203715935194307	23.40628895656702	21.79189500521863	27.59810010302004
18	26.900967757206327	21.427041746787104	23.39186164205382	28.280128853952746
19	28.403550021077407	22.146716771527693	22.918690811378642	26.531042396016257
20	29.3369296420448	21.063653762485828	22.795495071043252	26.803921524426123
21	27.92192568580465	22.021379476694253	22.01292597209667	28.043768865404427
22	28.684657227295578	22.66610009400297	21.391424314082638	27.257818364618814
23	28.065522550568865	22.08912022686952	22.23722562741911	27.608131595142503
24	28.183533474751073	21.693270784913537	22.391755691462862	27.73144004887253
25	27.977718816148673	21.73080434532679	22.911815294305946	27.379661544218592
26	28.132474306981692	22.389501423570174	22.402576177347765	27.07544809210037
27	27.606102754039085	21.68391557315888	23.44224452945539	27.267737143346643
28	26.582777844153444	22.502891098572373	22.354334844444242	28.55999621282994
29	27.589195744843924	22.97865433732414	22.434924921607834	26.9972249962241
30	27.073870104575487	21.676025635534476	23.609962060671368	27.64014219921867
31	27.646003295739657	22.263262421579654	22.66812893510639	27.422605347574297
32	26.040851842751287	22.199579353611224	22.978767050718773	28.780801752918713
33	26.403901686868664	21.854112799056814	24.29717562775725	27.444809886317273
34	26.6737375536234	22.00233091300104	23.59406947202792	27.72986206134765
35	26.297612955728432	23.121462208325912	24.017195555485422	26.56372928046023
36	26.583115984337347	22.053728220954323	24.20858289957462	27.15457289513371
37	26.493734262392277	21.96209223111656	24.34102113827003	27.203152368221133
38	26.245764794196614	21.040322089796508	25.21184482521534	27.50206829079154
39	25.260762438486665	20.69237584056014	26.173177368052063	27.87368435290113
40	25.410896680139672	21.29911204387707	26.659873806083368	26.63011746989989
41	23.925108712069125	20.65281343904347	27.583109221533668	27.83896862735374
42	22.63713275158193	21.35377804027475	28.219263620850178	27.789825587293144
43	22.298090860521683	21.316357193256135	28.14284393928806	28.24270800693413
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	7.0
2	9.0
3	25.5
4	42.0
5	42.0
6	58.0
7	74.0
8	80.5
9	87.0
10	132.0
11	177.0
12	177.0
13	344.0
14	511.0
15	973.5
16	1436.0
17	1427.0
18	1418.0
19	1418.0
20	1640.5
21	1863.0
22	1578.5
23	1294.0
24	1572.0
25	1850.0
26	1850.0
27	2260.5
28	2671.0
29	3445.0
30	4219.0
31	5466.5
32	6714.0
33	6714.0
34	9069.5
35	11425.0
36	13296.0
37	15167.0
38	19145.0
39	23123.0
40	23123.0
41	26624.5
42	30126.0
43	35920.5
44	41715.0
45	51754.5
46	61794.0
47	61794.0
48	67867.5
49	73941.0
50	82814.0
51	91687.0
52	93097.5
53	94508.0
54	94508.0
55	88296.0
56	82084.0
57	77885.0
58	73686.0
59	69913.0
60	66140.0
61	66140.0
62	62172.5
63	58205.0
64	49178.0
65	40151.0
66	35533.0
67	30915.0
68	30915.0
69	26661.5
70	22408.0
71	19451.0
72	16494.0
73	14256.5
74	12019.0
75	12019.0
76	10271.5
77	8524.0
78	6586.0
79	4648.0
80	3718.5
81	2789.0
82	2789.0
83	2186.5
84	1584.0
85	1229.0
86	874.0
87	681.0
88	488.0
89	488.0
90	366.5
91	245.0
92	158.5
93	72.0
94	46.5
95	21.0
96	21.0
97	11.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	887206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.15362010862263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.29260016642611	46.39917707540103
2	6.734501641689601	7.159262837665291
3	1.985653410636961	3.1663400116916365
4	0.893099122482212	1.8988580590305493
5	0.558676118846405	1.4847829092460763
6	0.3819666703703012	1.2181746774611086
7	0.2566340595555642	0.9548720515985067
8	0.18990082281332804	0.8075132955307598
9	0.16412041001746575	0.7851234533525783
>10	1.2496527356627172	13.699527414886806
>50	0.16489623657312927	6.136160842668317
>100	0.11744747913943474	11.633471290000328
>500	0.008085152547035825	2.7054216179535
>1k	0.002765973239775414	1.951314463513489
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1680	0.18935850298577783	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1664	0.18755508867162757	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1603	0.18067957159892967	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1447	0.1630962820359646	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1416	0.15960216680229847	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1368	0.15419192385984765	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1322	0.14900710770666564	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1245	0.1403281763198175	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1171	0.13198738511687252	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1130	0.12736613593686247	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1115	0.1256754350173466	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1061	0.11958891170708945	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1032	0.11632022326269209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1271339463439156E-4	0.0
3	0.0	0.0	0.0	1.1271339463439156E-4	0.0
4	0.0	0.0	0.0	1.1271339463439156E-4	0.0
5	0.0	0.0	0.0	1.1271339463439156E-4	0.0
6	0.0	0.0	0.0	1.1271339463439156E-4	0.0
7	0.0	0.0	0.0	1.1271339463439156E-4	0.0
8	1.1271339463439156E-4	0.0	0.0	1.1271339463439156E-4	0.0
9	1.1271339463439156E-4	0.0	0.0	2.2542678926878312E-4	0.0
10	1.1271339463439156E-4	0.0	0.0	2.2542678926878312E-4	0.0
11	1.1271339463439156E-4	0.0	0.0	3.381401839031747E-4	0.0
12	1.1271339463439156E-4	0.0	0.0	3.381401839031747E-4	0.0
13	1.1271339463439156E-4	0.0	0.0	3.381401839031747E-4	0.0
14	1.1271339463439156E-4	0.0	0.0	3.381401839031747E-4	0.0
15	1.1271339463439156E-4	0.0	0.0	5.635669731719578E-4	0.0
16	1.1271339463439156E-4	0.0	0.0	0.0011271339463439156	0.0
17	1.1271339463439156E-4	0.0	0.0	0.001577987524881482	0.0
18	2.2542678926878312E-4	0.0	0.0	0.0016907009195158734	0.0
19	2.2542678926878312E-4	0.0	0.0	0.0019161277087846565	0.0
20	2.2542678926878312E-4	0.0	0.0	0.002254267892687831	0.0
21	3.381401839031747E-4	0.0	0.0	0.004283108996106879	0.0
22	3.381401839031747E-4	0.0	0.0	0.005522956337085187	0.0
23	3.381401839031747E-4	0.0	0.0	0.006650090283429102	0.0
24	3.381401839031747E-4	0.0	0.0	0.008791644781482541	0.0
25	3.381401839031747E-4	0.0	0.0	0.009806065333192065	0.0
26	3.381401839031747E-4	0.0	0.0	0.011834906436611115	0.0
27	3.381401839031747E-4	0.0	0.0	0.03133432370836085	0.0
28	5.635669731719578E-4	0.0	0.0	0.11474223573781062	0.0
29	5.635669731719578E-4	0.0	0.0	0.2444753529619953	0.0
30	5.635669731719578E-4	0.0	0.0	0.40272495902868105	0.0
31	5.635669731719578E-4	0.0	0.0	0.8477174410452589	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAATTC	25	0.0054956754	29.6	25
GGTATCA	1130	0.0	28.81416	1
GTCTACG	45	1.3228801E-4	24.666666	1
TGTATAA	120	1.0913936E-11	21.583334	10
TACGCAT	45	0.003825131	20.555555	12
AGACCGT	45	0.003825131	20.555555	6
GTATCAA	1590	0.0	20.245285	2
TATAAGA	120	2.382876E-10	20.041668	12
TTATTAC	105	2.2577296E-8	19.380953	1
TCTTATA	1425	0.0	18.694736	37
GCGGTAA	680	0.0	18.5	23
AGACCGG	190	0.0	18.5	21
ATAAGAG	130	6.9485395E-10	18.5	13
ACTAAAC	60	9.2345517E-4	18.5	35
CGTGTAG	50	0.0070336475	18.5	8
GTATAAG	140	9.458745E-11	18.5	11
CTTATAC	705	0.0	18.368793	37
TAATACG	640	0.0	18.210938	27
CGCGGTA	715	0.0	18.111887	22
GTAACGT	410	0.0	18.048782	26
>>END_MODULE