FastQCFastQC Report
Fri 10 Feb 2017
ERR1631810.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631810.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669450
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53340.31950642427146664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48680.2915930396238282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42450.2542753601485519No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21320.12770672976129863No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA18530.11099463895294859No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18020.10793974063314266No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17790.10656204139087723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.027.4196431
TGCGGGT8050.018.38509221
GTATCAA34800.017.4899432
GTAACGT8200.017.37195226
TCTTATA29150.017.26243637
TTCTGCG8400.016.95833218
TAGTACC1452.9813236E-916.5862064
CGGGTAA8600.016.56395323
GGTAACG8650.016.46820825
CTTATAC15600.016.00961537
GCGGTAA6600.015.97727323
TAGACAG1403.475907E-815.8571435
TATTAGA2450.015.8571422
ATTAGAG2003.6379788E-1215.7253
CTCTATG5750.015.4434791
GTATATC2300.015.2826091
GCGGGTA9400.015.15425422
TATACAC6050.014.98347237
TTAGCCG9750.014.84
GTATAGC750.004105440314.81
Produced by FastQC (version 0.11.5)