##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631810.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1669450
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70176405402977	34.0	31.0	34.0	31.0	34.0
2	32.862694300518136	34.0	31.0	34.0	31.0	34.0
3	32.95030159633412	34.0	31.0	34.0	31.0	34.0
4	36.34691425319716	37.0	37.0	37.0	35.0	37.0
5	36.27218425229866	37.0	37.0	37.0	35.0	37.0
6	36.32742819491449	37.0	37.0	37.0	35.0	37.0
7	36.31229027524035	37.0	37.0	37.0	35.0	37.0
8	36.2982323519722	37.0	37.0	37.0	35.0	37.0
9	38.129642097696845	39.0	39.0	39.0	37.0	39.0
10	38.07349067058013	39.0	38.0	39.0	37.0	39.0
11	38.143018958339574	39.0	38.0	39.0	37.0	39.0
12	38.08735751295337	39.0	38.0	39.0	37.0	39.0
13	38.132559226092425	39.0	38.0	39.0	37.0	39.0
14	39.56462547545599	41.0	39.0	41.0	37.0	41.0
15	39.56266794453263	41.0	39.0	41.0	37.0	41.0
16	39.52574560483992	41.0	39.0	41.0	37.0	41.0
17	39.497367995447604	41.0	39.0	41.0	37.0	41.0
18	39.511506783671265	41.0	39.0	41.0	37.0	41.0
19	39.545457485998384	41.0	39.0	41.0	37.0	41.0
20	39.52831711042559	41.0	39.0	41.0	37.0	41.0
21	39.50104824942346	41.0	39.0	41.0	37.0	41.0
22	39.479026026535685	41.0	39.0	41.0	37.0	41.0
23	39.412883284914194	41.0	39.0	41.0	37.0	41.0
24	39.429085028003236	41.0	39.0	41.0	37.0	41.0
25	39.37376920542694	41.0	39.0	41.0	36.0	41.0
26	39.32140824822547	41.0	39.0	41.0	36.0	41.0
27	39.2258863697625	40.0	39.0	41.0	36.0	41.0
28	39.207518044865076	40.0	39.0	41.0	36.0	41.0
29	39.183791068914914	40.0	39.0	41.0	36.0	41.0
30	39.13139117673485	40.0	39.0	41.0	35.0	41.0
31	39.04204079187756	40.0	39.0	41.0	35.0	41.0
32	38.979308754380185	40.0	39.0	41.0	35.0	41.0
33	38.91881997064902	40.0	38.0	41.0	35.0	41.0
34	38.88232411872173	40.0	38.0	41.0	35.0	41.0
35	38.81157686663272	40.0	38.0	41.0	35.0	41.0
36	38.758635478750485	40.0	38.0	41.0	35.0	41.0
37	38.70815657851388	40.0	38.0	41.0	35.0	41.0
38	38.66324717721405	40.0	38.0	41.0	35.0	41.0
39	38.590922159992814	40.0	38.0	41.0	35.0	41.0
40	38.2605756386834	40.0	38.0	41.0	34.0	41.0
41	38.322216298781036	40.0	38.0	41.0	34.0	41.0
42	38.2926167300608	40.0	38.0	41.0	34.0	41.0
43	37.8392596364072	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	0.0
13	1.0
14	0.0
15	4.0
16	3.0
17	9.0
18	25.0
19	39.0
20	64.0
21	133.0
22	244.0
23	480.0
24	694.0
25	1263.0
26	1947.0
27	3155.0
28	4757.0
29	7096.0
30	10410.0
31	14125.0
32	18994.0
33	26359.0
34	38368.0
35	58085.0
36	90828.0
37	168232.0
38	369604.0
39	854528.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82033004881847	17.287489891880558	12.902213303782684	26.989966755518285
2	19.508101470544194	20.279553146245767	34.177783102219294	26.034562280990748
3	20.522627212555033	21.271316900775705	29.70948515978316	28.4965707268861
4	15.177034352631106	16.219533379256642	34.278055647069394	34.32537662104286
5	16.23762316930726	33.87462936895385	33.70307586330827	16.184671598430622
6	33.539189553445745	34.32855131929677	15.846416484470934	16.285842642786548
7	29.581658630087755	28.924675791428317	20.95588367426398	20.537781904219955
8	26.838359938902034	32.306088831651145	20.28781934169936	20.567731887747463
9	27.635269100601995	13.942675731528348	18.87891221659828	39.54314295127138
10	18.412590973074966	25.026625535355958	31.233639821498098	25.327143670070985
11	36.70640031147983	20.979604061217767	20.45679714876157	21.857198478540838
12	22.301236934319686	24.78924196591692	27.92907843900686	24.980442660756534
13	32.50579532181258	18.938872083620353	22.995597352421456	25.559735242145614
14	23.452035101380694	20.696816316751026	24.6612956362874	31.18985294558088
15	27.539309353379853	25.71709245559915	21.779208721435204	24.96438946958579
16	24.788583066279312	25.328940669082634	23.745185540147954	26.137290724490104
17	25.13139057773518	25.046452424451164	24.010182994399354	25.8119740034143
18	24.770792776063974	23.520201263889305	25.613345712659857	26.095660247386864
19	26.28602234268771	23.794722812902453	25.675402078528858	24.24385276588098
20	27.117134385576087	22.96948096678547	25.3273832699392	24.586001377699244
21	25.452873700919465	24.110934738985893	24.51004821947348	25.92614334062116
22	25.959088322501422	24.41594537123005	24.088292551439096	25.536673754829437
23	25.737817844200183	24.042708676510227	24.91077899907155	25.308694480218037
24	25.941717332055468	23.80017370990446	24.833687741471742	25.424421216568334
25	25.837131989577404	23.734283746143937	25.220521728713045	25.208062535565606
26	25.698104166042707	24.317829225193925	24.738267093953098	25.24579951481027
27	25.591542124651834	23.643655095989697	25.26562640390548	25.499176375452993
28	24.826799245260414	24.351792506514123	24.845547934948637	25.975860313276826
29	25.313007277845994	24.767558177843004	24.71688280571446	25.202551738596547
30	24.994159753212138	24.342627811554703	25.54068705262212	25.122525382611038
31	25.67390457935248	24.159693312168677	24.984396058582167	25.18200604989667
32	23.871155170864654	24.24936356285004	25.31073107909791	26.568750187187394
33	24.211327083770104	23.870196771391775	26.411752373536196	25.506723771301925
34	24.748150588517177	23.58321602923118	25.961424421216567	25.70720896103507
35	24.54682679924526	24.441043457426098	26.047620473808742	24.9645092695199
36	24.549222797927463	23.759441732307046	26.35694390368085	25.33439156608464
37	24.926293090538802	23.074964808769355	26.09811614603612	25.900625954655727
38	24.228158974512564	22.851717631555303	27.135343975560815	25.78477941837132
39	23.85366438048459	22.186408697475215	27.826349995507506	26.133576926532694
40	23.49067058013118	22.786546467399443	28.38485728832849	25.337925664140887
41	22.356584503878523	22.297882536164604	29.337566264338555	26.007966695618318
42	21.64868669322232	22.645242445116658	29.39495043277726	26.311120428883765
43	21.093473898589355	22.911737398544428	29.47156249064063	26.52322621222558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	96.5
2	111.0
3	242.0
4	373.0
5	373.0
6	476.5
7	580.0
8	637.0
9	694.0
10	1101.5
11	1509.0
12	1509.0
13	2839.5
14	4170.0
15	7428.5
16	10687.0
17	10571.5
18	10456.0
19	10456.0
20	11766.5
21	13077.0
22	11744.5
23	10412.0
24	11246.0
25	12080.0
26	12080.0
27	13625.5
28	15171.0
29	19400.0
30	23629.0
31	27110.5
32	30592.0
33	30592.0
34	35529.0
35	40466.0
36	44686.5
37	48907.0
38	56681.5
39	64456.0
40	64456.0
41	72617.0
42	80778.0
43	86517.0
44	92256.0
45	106470.0
46	120684.0
47	120684.0
48	128872.0
49	137060.0
50	150661.5
51	164263.0
52	166248.5
53	168234.0
54	168234.0
55	152501.0
56	136768.0
57	126938.5
58	117109.0
59	106684.0
60	96259.0
61	96259.0
62	88408.5
63	80558.0
64	68237.0
65	55916.0
66	48446.5
67	40977.0
68	40977.0
69	35268.5
70	29560.0
71	25799.5
72	22039.0
73	18226.0
74	14413.0
75	14413.0
76	11838.5
77	9264.0
78	7793.0
79	6322.0
80	5049.0
81	3776.0
82	3776.0
83	3136.5
84	2497.0
85	2056.0
86	1615.0
87	1305.5
88	996.0
89	996.0
90	738.0
91	480.0
92	309.0
93	138.0
94	82.0
95	26.0
96	26.0
97	17.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1669450.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.32769767387067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.86123535435127	43.71525992492716
2	6.530029257108826	6.572826765066065
3	2.1419613250108576	3.2339994598280963
4	1.0554967934439845	2.1248289406475505
5	0.6336098052173236	1.5944061360088766
6	0.42844716033981955	1.2937655492886497
7	0.29878712136138075	1.0526087538905171
8	0.2438988433752233	0.9819893801915976
9	0.17947046101247952	0.8129101592903634
>10	1.2888908263182584	12.771849664792278
>50	0.1864020725363889	6.634516827640994
>100	0.13970110214959508	13.555686762545971
>500	0.009560299227405698	3.2141277760610847
>1k	0.0023900748068514245	2.1204185713742185
>5k	1.1950374034257122E-4	0.3208053284465079
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5334	0.31950642427146664	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4868	0.2915930396238282	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4245	0.2542753601485519	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2132	0.12770672976129863	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1853	0.11099463895294859	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1802	0.10793974063314266	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1779	0.10656204139087723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.989996705501812E-5	0.0
8	5.989996705501812E-5	0.0	0.0	5.989996705501812E-5	0.0
9	5.989996705501812E-5	0.0	0.0	1.1979993411003624E-4	0.0
10	1.1979993411003624E-4	0.0	0.0	1.1979993411003624E-4	5.989996705501812E-5
11	1.1979993411003624E-4	0.0	0.0	1.1979993411003624E-4	5.989996705501812E-5
12	1.1979993411003624E-4	0.0	0.0	1.7969990116505435E-4	5.989996705501812E-5
13	1.1979993411003624E-4	0.0	0.0	2.994998352750906E-4	5.989996705501812E-5
14	1.1979993411003624E-4	0.0	0.0	2.994998352750906E-4	5.989996705501812E-5
15	1.1979993411003624E-4	0.0	0.0	4.7919973644014495E-4	1.1979993411003624E-4
16	1.1979993411003624E-4	0.0	0.0	0.0014375992093204348	1.1979993411003624E-4
17	1.1979993411003624E-4	0.0	0.0	0.002036598879870616	1.7969990116505435E-4
18	1.1979993411003624E-4	0.0	5.989996705501812E-5	0.0023959986822007246	1.7969990116505435E-4
19	1.1979993411003624E-4	0.0	5.989996705501812E-5	0.0028751984186408696	1.7969990116505435E-4
20	1.7969990116505435E-4	0.0	5.989996705501812E-5	0.0036538979903561054	1.7969990116505435E-4
21	2.994998352750906E-4	0.0	5.989996705501812E-5	0.005450897002006649	1.7969990116505435E-4
22	2.994998352750906E-4	0.0	5.989996705501812E-5	0.007187996046602175	1.7969990116505435E-4
23	2.994998352750906E-4	5.989996705501812E-5	5.989996705501812E-5	0.008805295157087663	1.7969990116505435E-4
24	2.994998352750906E-4	5.989996705501812E-5	5.989996705501812E-5	0.010662194135793225	1.7969990116505435E-4
25	2.994998352750906E-4	5.989996705501812E-5	5.989996705501812E-5	0.011620593608673515	1.7969990116505435E-4
26	2.994998352750906E-4	5.989996705501812E-5	5.989996705501812E-5	0.015214591631974602	1.7969990116505435E-4
27	3.593998023301087E-4	5.989996705501812E-5	5.989996705501812E-5	0.037976579112881485	1.7969990116505435E-4
28	4.1929976938512683E-4	1.1979993411003624E-4	5.989996705501812E-5	0.12153703315463177	1.7969990116505435E-4
29	4.7919973644014495E-4	1.1979993411003624E-4	5.989996705501812E-5	0.2456497648926293	1.7969990116505435E-4
30	4.7919973644014495E-4	1.7969990116505435E-4	5.989996705501812E-5	0.4069603761717931	1.7969990116505435E-4
31	4.7919973644014495E-4	1.7969990116505435E-4	5.989996705501812E-5	0.8753182185749798	1.7969990116505435E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2240	0.0	27.419643	1
TGCGGGT	805	0.0	18.385092	21
GTATCAA	3480	0.0	17.489943	2
GTAACGT	820	0.0	17.371952	26
TCTTATA	2915	0.0	17.262436	37
TTCTGCG	840	0.0	16.958332	18
TAGTACC	145	2.9813236E-9	16.586206	4
CGGGTAA	860	0.0	16.563953	23
GGTAACG	865	0.0	16.468208	25
CTTATAC	1560	0.0	16.009615	37
GCGGTAA	660	0.0	15.977273	23
TAGACAG	140	3.475907E-8	15.857143	5
TATTAGA	245	0.0	15.857142	2
ATTAGAG	200	3.6379788E-12	15.725	3
CTCTATG	575	0.0	15.443479	1
GTATATC	230	0.0	15.282609	1
GCGGGTA	940	0.0	15.154254	22
TATACAC	605	0.0	14.983472	37
TTAGCCG	975	0.0	14.8	4
GTATAGC	75	0.0041054403	14.8	1
>>END_MODULE