FastQCFastQC Report
Fri 10 Feb 2017
ERR1631809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556348
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14310.25721311121815843No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13700.2462487507818847No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13030.2342059286633546No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12010.21587208006499528No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11570.20796336106178145No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11570.20796336106178145No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11140.20023438567227705No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10830.19466233364728552No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10670.19178643582793503No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9300.16716156074974656No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8890.15979207258766095No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8110.14577207071832737No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7840.14091899314817347No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6540.11755232336595081No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6500.11683334891111319No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6370.11449668193289092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6210.11162078411354046No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5990.10766642461193354No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5910.1062284757022583No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5870.10550950124742069No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5740.10317283426919842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.10029693644984794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGTA250.005493936629.5999987
GGTATCA7350.028.945581
TTAGGAT358.8636216E-426.428573
ATACGGC706.5774657E-926.4285729
TACAGAG400.001929941623.1257
TTTTACG400.001929941623.1254
GGTAATA5200.022.7692325
ACCATTG753.733585E-722.28
TTATACT502.6996748E-422.1999994
GCGGTAA5400.021.58333423
CGGTAAT5500.021.19090824
TAATACG5450.021.04587227
CGCGGTA5800.020.73275822
CCTAGAC450.003823363220.5555573
TACGGCT909.456198E-820.55555730
AGCCGCG5850.020.55555519
ATACTTC555.13851E-420.1818186
TGCGGGT3600.020.04166821
GTAATAC5850.019.92307726
AACGACC759.251686E-619.73333437
Produced by FastQC (version 0.11.5)