##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631809.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556348
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52875897819351	34.0	31.0	34.0	31.0	34.0
2	32.70393171180628	34.0	31.0	34.0	31.0	34.0
3	32.79288682623106	34.0	31.0	34.0	31.0	34.0
4	36.240083544831656	37.0	37.0	37.0	35.0	37.0
5	36.14318376268091	37.0	35.0	37.0	35.0	37.0
6	36.19756878788097	37.0	36.0	37.0	35.0	37.0
7	36.196310582585	37.0	36.0	37.0	35.0	37.0
8	36.172674297382216	37.0	36.0	37.0	35.0	37.0
9	37.99032080640175	39.0	38.0	39.0	35.0	39.0
10	37.91009224442256	39.0	38.0	39.0	35.0	39.0
11	37.9914280270622	39.0	38.0	39.0	35.0	39.0
12	37.923452587229576	39.0	38.0	39.0	35.0	39.0
13	37.97834628685643	39.0	38.0	39.0	35.0	39.0
14	39.34531624091396	40.0	39.0	41.0	36.0	41.0
15	39.35198472898258	40.0	39.0	41.0	36.0	41.0
16	39.290537577199885	40.0	39.0	41.0	36.0	41.0
17	39.256458188040575	40.0	39.0	41.0	36.0	41.0
18	39.28600444326213	40.0	39.0	41.0	36.0	41.0
19	39.30149474789161	40.0	39.0	41.0	36.0	41.0
20	39.29692386779498	40.0	39.0	41.0	36.0	41.0
21	39.25838863445182	40.0	39.0	41.0	36.0	41.0
22	39.236927246974915	40.0	39.0	41.0	36.0	41.0
23	39.172000258830806	40.0	39.0	41.0	36.0	41.0
24	39.18447626305837	40.0	39.0	41.0	36.0	41.0
25	39.13190485092065	40.0	39.0	41.0	36.0	41.0
26	39.061916282614476	40.0	39.0	41.0	36.0	41.0
27	38.95064060623926	40.0	38.0	41.0	35.0	41.0
28	38.94287747956315	40.0	38.0	41.0	35.0	41.0
29	38.90832536469979	40.0	38.0	41.0	35.0	41.0
30	38.87700683744706	40.0	38.0	41.0	35.0	41.0
31	38.79264417235255	40.0	38.0	41.0	35.0	41.0
32	38.7394220883332	40.0	38.0	41.0	35.0	41.0
33	38.67714991336358	40.0	38.0	41.0	35.0	41.0
34	38.6539306333446	40.0	38.0	41.0	35.0	41.0
35	38.58290494438732	40.0	38.0	41.0	35.0	41.0
36	38.541339233717025	40.0	38.0	41.0	35.0	41.0
37	38.486891298252175	40.0	38.0	41.0	34.0	41.0
38	38.43848095077182	40.0	38.0	41.0	34.0	41.0
39	38.36217978675218	40.0	38.0	41.0	34.0	41.0
40	38.04111635163603	40.0	37.0	41.0	34.0	41.0
41	38.126059588602814	40.0	37.0	41.0	34.0	41.0
42	38.1111390712288	40.0	37.0	41.0	34.0	41.0
43	37.61609280522263	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	3.0
19	13.0
20	23.0
21	34.0
22	106.0
23	174.0
24	329.0
25	502.0
26	838.0
27	1270.0
28	2004.0
29	2810.0
30	4071.0
31	5532.0
32	7408.0
33	10070.0
34	14864.0
35	22223.0
36	34238.0
37	61917.0
38	144541.0
39	243374.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.630691581528104	16.53030117839913	12.906310438790108	27.932696801282653
2	20.465428113339133	19.826799053829617	32.98421132097177	26.723561511859483
3	21.05300998655518	19.963404200248767	29.469324954884353	29.5142608583117
4	15.458490009849951	16.24990114101246	34.27423123656416	34.017377612573426
5	16.534615025128158	32.97109003717098	33.94098657674693	16.553308360953935
6	33.58149934932812	33.95734324559449	15.862913140696111	16.598244264381286
7	29.88453270255308	27.958759625270513	20.443858879694005	21.712848792482404
8	27.04152796451142	30.890737452098328	20.772969436395925	21.294765146994326
9	28.34359070222235	13.070775845334214	17.954230086205037	40.6314033662384
10	18.934012524535003	23.897093186279093	30.105078116574518	27.06381617261139
11	38.43529589393689	19.89294470367468	19.171993069086255	22.49976633330218
12	22.431463760092605	24.189715789398004	27.031102834916275	26.34771761559312
13	34.71514232099334	18.015882145707362	21.225743599329917	26.043231933969384
14	23.908237290329073	20.53822427689144	23.397945171008075	32.15559326177141
15	29.269809543666913	24.500492497501565	20.283347832651504	25.94635012618002
16	25.126539504051422	24.451602234572604	22.742060724582455	27.679797536793515
17	26.52224866450495	24.212543228339097	22.10918346071164	27.15602464644431
18	26.259104014034378	21.763895978775874	23.890083185344423	28.08691682184532
19	27.727070107199093	22.78897380776061	23.847483948895295	25.636472136145006
20	29.554163940555195	21.242998986246022	23.289200284713885	25.913636788484908
21	26.89719384270277	22.653806610251138	22.302048358221832	28.14695118882426
22	27.93862834053506	23.08159641087952	21.897265740148253	27.082509508437163
23	27.311143385075525	22.5899976273843	22.871835613680645	27.227023373859527
24	27.47560879161963	21.942021899961894	23.440903894684624	27.14146541373385
25	27.4653634056382	22.317326565387134	23.40585389001129	26.81145613896338
26	27.450624429314026	23.131924622718156	22.749430212744542	26.66802073522328
27	26.798514598776308	21.972398570678784	24.340161194072774	26.888925636472138
28	25.940778074155023	23.158706421160858	22.5689676245803	28.33154788010382
29	26.813433318714186	23.575352117739257	22.83121355698232	26.780001006564234
30	26.517755074162213	22.672499946076915	23.860605232696084	26.949139747064788
31	27.70891600221444	22.706291745454283	23.105322567889164	26.479469684442115
32	25.441090828042878	22.724445850438933	23.337731060415422	28.496732261102764
33	25.66487162711109	22.375563496228978	24.805876897193844	27.153687979466092
34	26.064801167614515	22.397851704328943	24.025250382853898	27.51209674520264
35	25.603399311222475	24.009432944847468	24.437582232703274	25.949585511226786
36	25.946170382566308	22.950024085644237	24.837691516820406	26.266114014969048
37	26.823139473854496	22.692810974426077	24.34088016852761	26.143169383191818
38	25.820529596583437	22.40162632021684	25.214793618382743	26.563050464816985
39	25.532760071034676	21.47540747877228	25.86438703832853	27.127445411864514
40	25.076930266667624	22.32703272052744	26.522967638959788	26.073069373845147
41	23.466966718672484	21.746460848246063	27.449366224018064	27.337206209063392
42	22.530861978473904	21.869585223637003	28.10147605455578	27.49807674333331
43	21.92153112799902	22.697484308382524	27.871044741780327	27.50993982183813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	6.0
2	7.0
3	16.0
4	25.0
5	25.0
6	37.0
7	49.0
8	59.5
9	70.0
10	100.5
11	131.0
12	131.0
13	262.5
14	394.0
15	706.5
16	1019.0
17	1053.5
18	1088.0
19	1088.0
20	1278.0
21	1468.0
22	1333.0
23	1198.0
24	1394.0
25	1590.0
26	1590.0
27	1924.0
28	2258.0
29	3090.5
30	3923.0
31	4627.5
32	5332.0
33	5332.0
34	6427.0
35	7522.0
36	8497.5
37	9473.0
38	12074.5
39	14676.0
40	14676.0
41	17166.0
42	19656.0
43	22037.0
44	24418.0
45	33537.5
46	42657.0
47	42657.0
48	46580.5
49	50504.0
50	57811.0
51	65118.0
52	66265.5
53	67413.0
54	67413.0
55	59908.0
56	52403.0
57	49488.5
58	46574.0
59	42601.0
60	38628.0
61	38628.0
62	35341.0
63	32054.0
64	26580.0
65	21106.0
66	18133.0
67	15160.0
68	15160.0
69	12931.5
70	10703.0
71	9208.0
72	7713.0
73	6171.0
74	4629.0
75	4629.0
76	3751.0
77	2873.0
78	2407.5
79	1942.0
80	1505.0
81	1068.0
82	1068.0
83	883.0
84	698.0
85	534.5
86	371.0
87	291.5
88	212.0
89	212.0
90	183.5
91	155.0
92	104.5
93	54.0
94	29.5
95	5.0
96	5.0
97	5.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	556348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.53780663615449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93116519022921	43.06379251849179
2	6.262217530394929	6.2043304226848175
3	1.990077744317251	2.9575225946670747
4	1.065141979957376	2.1105918977271694
5	0.6158058766825373	1.5252836222253567
6	0.4290825313100479	1.2753484480193316
7	0.3312659998739205	1.1487133732820656
8	0.24762464853118205	0.981342556582673
9	0.19279053731814116	0.8595378323051763
>10	1.5860796306504288	16.17510676756505
>50	0.20757092073945202	7.155177266174883
>100	0.13059822423356834	12.302444879802005
>500	0.007295990180646276	2.2741008911589584
>1k	0.0032831955812908245	1.9667069293136479
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1431	0.25721311121815843	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1370	0.2462487507818847	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1303	0.2342059286633546	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1201	0.21587208006499528	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1157	0.20796336106178145	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1157	0.20796336106178145	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1114	0.20023438567227705	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1083	0.19466233364728552	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1067	0.19178643582793503	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	930	0.16716156074974656	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	889	0.15979207258766095	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	811	0.14577207071832737	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	784	0.14091899314817347	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	654	0.11755232336595081	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	650	0.11683334891111319	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	637	0.11449668193289092	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	621	0.11162078411354046	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	599	0.10766642461193354	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	591	0.1062284757022583	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	587	0.10550950124742069	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	574	0.10317283426919842	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	558	0.10029693644984794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.797436137094049E-4
2	0.0	0.0	0.0	0.0	1.797436137094049E-4
3	0.0	0.0	0.0	0.0	1.797436137094049E-4
4	0.0	0.0	0.0	0.0	1.797436137094049E-4
5	0.0	0.0	0.0	0.0	1.797436137094049E-4
6	0.0	0.0	0.0	0.0	1.797436137094049E-4
7	0.0	0.0	0.0	0.0	1.797436137094049E-4
8	0.0	0.0	0.0	0.0	1.797436137094049E-4
9	0.0	0.0	0.0	0.0	1.797436137094049E-4
10	0.0	0.0	0.0	0.0	1.797436137094049E-4
11	0.0	0.0	0.0	0.0	3.594872274188098E-4
12	0.0	0.0	0.0	0.0	3.594872274188098E-4
13	3.594872274188098E-4	0.0	0.0	0.0	3.594872274188098E-4
14	3.594872274188098E-4	0.0	0.0	0.0	3.594872274188098E-4
15	3.594872274188098E-4	0.0	0.0	0.0	3.594872274188098E-4
16	3.594872274188098E-4	0.0	0.0	1.797436137094049E-4	3.594872274188098E-4
17	3.594872274188098E-4	0.0	0.0	7.189744548376197E-4	3.594872274188098E-4
18	3.594872274188098E-4	0.0	0.0	7.189744548376197E-4	3.594872274188098E-4
19	3.594872274188098E-4	0.0	0.0	0.0012582052959658344	3.594872274188098E-4
20	3.594872274188098E-4	0.0	0.0	0.0017974361370940491	3.594872274188098E-4
21	3.594872274188098E-4	0.0	0.0	0.0032353850467692884	3.594872274188098E-4
22	3.594872274188098E-4	0.0	0.0	0.006650513707247982	3.594872274188098E-4
23	3.594872274188098E-4	0.0	0.0	0.007549231775795006	3.594872274188098E-4
24	3.594872274188098E-4	0.0	0.0	0.009346667912889055	3.594872274188098E-4
25	3.594872274188098E-4	0.0	0.0	0.00952641152659846	3.594872274188098E-4
26	3.594872274188098E-4	0.0	0.0	0.011323847663692509	3.594872274188098E-4
27	3.594872274188098E-4	0.0	0.0	0.023546413395932043	3.594872274188098E-4
28	3.594872274188098E-4	0.0	0.0	0.08376052398858269	3.594872274188098E-4
29	3.594872274188098E-4	0.0	0.0	0.1865738710303623	3.594872274188098E-4
30	3.594872274188098E-4	0.0	0.0	0.31616901651484325	3.594872274188098E-4
31	5.392308411282147E-4	0.0	0.0	0.6981241956473286	3.594872274188098E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTGTA	25	0.0054939366	29.599998	7
GGTATCA	735	0.0	28.94558	1
TTAGGAT	35	8.8636216E-4	26.42857	3
ATACGGC	70	6.5774657E-9	26.42857	29
TACAGAG	40	0.0019299416	23.125	7
TTTTACG	40	0.0019299416	23.125	4
GGTAATA	520	0.0	22.76923	25
ACCATTG	75	3.733585E-7	22.2	8
TTATACT	50	2.6996748E-4	22.199999	4
GCGGTAA	540	0.0	21.583334	23
CGGTAAT	550	0.0	21.190908	24
TAATACG	545	0.0	21.045872	27
CGCGGTA	580	0.0	20.732758	22
CCTAGAC	45	0.0038233632	20.555557	3
TACGGCT	90	9.456198E-8	20.555557	30
AGCCGCG	585	0.0	20.555555	19
ATACTTC	55	5.13851E-4	20.181818	6
TGCGGGT	360	0.0	20.041668	21
GTAATAC	585	0.0	19.923077	26
AACGACC	75	9.251686E-6	19.733334	37
>>END_MODULE