ERR1631808.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631808.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30228
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	78	0.25803890432711396	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	74	0.24480614000264656	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	66	0.21834061135371177	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	57	0.1885668916236602	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	56	0.18525870054254334	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	49	0.16210136297472544	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	47	0.15548498081249174	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	46	0.15217678973137488	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	46	0.15217678973137488	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	45	0.14886859865025803	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	43	0.14225221648802433	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	41	0.13563583432579066	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	38	0.12571126108244013	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	37	0.12240307000132328	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	37	0.12240307000132328	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	36	0.11909487892020643	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	36	0.11909487892020643	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	34	0.11247849675797275	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	33	0.10917030567685589	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	33	0.10917030567685589	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	32	0.10586211459573905	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	32	0.10586211459573905	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	31	0.1025539235146222	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAGGG	20	0.0018137058	37.0	32
TACGGAG	20	0.0018137058	37.0	30
ATACGGA	20	0.0018137058	37.0	29
TAATACG	20	0.0018137058	37.0	27
AATACGG	20	0.0018137058	37.0	28
GTAATAC	20	0.0018137058	37.0	26
ACGGAGG	20	0.0018137058	37.0	31
CCCTGTC	30	3.5178717E-4	30.833334	36
GGTATCA	75	1.2732926E-11	29.6	1
GCGGTAA	25	0.005413063	29.599998	23
CGGTAAT	25	0.005413063	29.599998	24
GGTAATA	25	0.005413063	29.599998	25
GTATCAA	80	2.910383E-11	27.750002	2
TACGGGC	35	8.6701906E-4	26.428572	16
AGCCGCG	40	0.0018882208	23.125002	19
TCAACGC	100	4.765752E-10	22.199999	5
CGCAGAG	100	4.765752E-10	22.199999	9
ATCAACG	100	4.765752E-10	22.199999	4
CAACGCA	100	4.765752E-10	22.199999	6
AACGCAG	105	8.749339E-10	21.142857	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content