FastQCFastQC Report
Fri 10 Feb 2017
ERR1631807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128356
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6430.5009504814734022No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5780.4503100751036181No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5520.43005391255570447No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5050.39343700333447595No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4650.3622736763376858No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4430.3451338464894512No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4370.34045934743993267No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4010.3124123531428215No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4000.31163326996790175No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3680.2867026083704696No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3650.28436535884571035No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3580.2789117766212721No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2760.21502695627785223No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2730.21268970675309296No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2530.19710804325469788No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2500.1947707937299386No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2470.19243354420517936No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2440.19009629468042008No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2410.18775904515566083No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2390.1862008788058213No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2310.17996821340646327No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2260.1760727975318645No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2140.16672379943282745No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2140.16672379943282745No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2060.1604911340334694No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2040.1589329676836299No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2010.15659571815887066No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2000.15581663498395087No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1950.15192121910935213No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1950.15192121910935213No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1930.1503630527595126No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1900.14802580323475334No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1900.14802580323475334No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1880.14646763688491382No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1860.14490947053507433No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1810.14101405466047554No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1810.14101405466047554No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1720.13400230608619776No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1710.133223222911278No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1670.130106890211599No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1650.12854872386175947No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1650.12854872386175947No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1640.12776964068683974No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1630.12699055751191995No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1630.12699055751191995No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1590.12387422481224095No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1580.1230951416373212No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1570.12231605846240144No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1560.1215369752874817No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1560.1215369752874817No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1540.11997880893764219No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1510.11764155941288292No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1500.11686247623796317No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1500.11686247623796317No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1480.11530430988812367No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1480.11530430988812367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1470.11452522671320389No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1470.11452522671320389No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1440.11218797718844464No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1430.11140889401352488No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1430.11140889401352488No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1350.10517622861416684No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1340.10439714543924711No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1330.10361806226432733No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1330.10361806226432733No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC1290.10050172956464831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACC200.001835851837.04
GAGCAGA200.001835851837.01
AAAGACT303.581846E-430.83333436
AGAACCC250.00547839529.65
GATATGC706.4756023E-926.4285734
ATATGCC659.256655E-825.61538535
CTTATTG809.586074E-1025.437528
TATGCCC601.3220069E-624.66666836
GGTATCA2850.024.6666661
TTATTGA851.9354047E-923.94117729
CTCTATG1350.023.2962971
GTACTGC400.00192190623.1256
GTGGTCA400.00192190623.12529
CAGTCGG903.748937E-922.61111310
GTCGGTG903.748937E-922.61111312
AGTCGGT903.748937E-922.61111311
GTCAATG1155.456968E-1222.52173831
AATGAGC1005.2204996E-1022.234
TCAATGA1107.2759576E-1121.86363632
CGTCAAT1209.094947E-1221.58333430
Produced by FastQC (version 0.11.5)