##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631807.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128356
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.597712611798435	34.0	31.0	34.0	31.0	34.0
2	32.76529340272368	34.0	31.0	34.0	31.0	34.0
3	32.84914612484029	34.0	31.0	34.0	31.0	34.0
4	36.26945370687775	37.0	37.0	37.0	35.0	37.0
5	36.19601732680981	37.0	35.0	37.0	35.0	37.0
6	36.25704291190127	37.0	37.0	37.0	35.0	37.0
7	36.259808657172236	37.0	37.0	37.0	35.0	37.0
8	36.23695814765184	37.0	37.0	37.0	35.0	37.0
9	38.08892455358534	39.0	38.0	39.0	37.0	39.0
10	38.00377076256661	39.0	38.0	39.0	35.0	39.0
11	38.09120726728786	39.0	38.0	39.0	37.0	39.0
12	37.99641621739537	39.0	38.0	39.0	35.0	39.0
13	38.074410234036584	39.0	38.0	39.0	37.0	39.0
14	39.42893203278382	40.0	39.0	41.0	37.0	41.0
15	39.45567795817882	41.0	39.0	41.0	37.0	41.0
16	39.37674125089595	40.0	39.0	41.0	36.0	41.0
17	39.355394371903145	40.0	39.0	41.0	36.0	41.0
18	39.38679921468416	40.0	39.0	41.0	37.0	41.0
19	39.40278600143351	40.0	39.0	41.0	37.0	41.0
20	39.40556732836798	40.0	39.0	41.0	37.0	41.0
21	39.35037707625666	40.0	39.0	41.0	36.0	41.0
22	39.32162111627037	40.0	39.0	41.0	36.0	41.0
23	39.25659883449157	40.0	39.0	41.0	36.0	41.0
24	39.273699710181056	40.0	39.0	41.0	36.0	41.0
25	39.19978808937642	40.0	39.0	41.0	36.0	41.0
26	39.12934338870018	40.0	39.0	41.0	36.0	41.0
27	39.001184206425876	40.0	39.0	41.0	35.0	41.0
28	39.0131119698339	40.0	39.0	41.0	35.0	41.0
29	38.98657639689613	40.0	39.0	41.0	35.0	41.0
30	38.94544080526037	40.0	38.0	41.0	35.0	41.0
31	38.855830658481096	40.0	38.0	41.0	35.0	41.0
32	38.792514568855374	40.0	38.0	41.0	35.0	41.0
33	38.690836423696595	40.0	38.0	41.0	35.0	41.0
34	38.686161924647074	40.0	38.0	41.0	35.0	41.0
35	38.60818348966936	40.0	38.0	41.0	35.0	41.0
36	38.561329427529685	40.0	38.0	41.0	35.0	41.0
37	38.51841752625511	40.0	38.0	41.0	34.0	41.0
38	38.47698588301287	40.0	38.0	41.0	34.0	41.0
39	38.36301380535386	40.0	38.0	41.0	34.0	41.0
40	38.051411698712954	40.0	37.0	41.0	33.0	41.0
41	38.128026738134565	40.0	37.0	41.0	34.0	41.0
42	38.12671008756895	40.0	37.0	41.0	34.0	41.0
43	37.634742435102375	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	0.0
18	0.0
19	1.0
20	6.0
21	6.0
22	21.0
23	36.0
24	76.0
25	110.0
26	185.0
27	277.0
28	430.0
29	609.0
30	884.0
31	1264.0
32	1631.0
33	2297.0
34	3346.0
35	5033.0
36	7668.0
37	13519.0
38	31033.0
39	59922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59793075508742	16.242326030727043	12.769173236934774	28.390569977250774
2	21.429461809342765	19.54797594191156	32.00395774252859	27.01860450621708
3	21.21521393623983	19.82844588488267	29.615288728224627	29.341051450652873
4	15.073701268347408	16.565645548318738	36.78597027018605	31.574682913147807
5	16.475271900028048	32.63735236373835	34.44015083050267	16.447224905730938
6	30.84390289507308	35.734207984044374	16.575773629592696	16.84611549128985
7	28.604038767178785	27.774315185889247	20.84437034497803	22.777275701953943
8	26.216148836049737	29.54906665835645	21.584499361151796	22.65028514444202
9	28.691296082769796	12.113964286827262	18.359874100158933	40.83486553024401
10	19.98971610209106	22.058961014677926	28.726354825641188	29.224968057589827
11	39.24475677023279	19.230110006544297	18.562435725638068	22.96269749758484
12	22.05039109975381	24.039390445323942	26.7038549035495	27.206363551372743
13	35.92897877777432	17.560534762691265	19.944529277945712	26.565957181588708
14	23.70516376328337	20.764903861136215	23.44261273333541	32.087319642245006
15	31.070616098974728	23.08423447287232	20.162672566923245	25.682476861229702
16	24.30739505749634	24.158590171086665	23.293807846925738	28.240206924491257
17	26.893172115055002	23.93187696718502	20.819439683380597	28.35551123437938
18	26.311976066564867	20.877870921499582	23.145782043690986	29.66437096824457
19	27.711988531895663	22.294244133503692	23.73087350805572	26.262893826544918
20	31.231886316183115	19.748979401040856	22.479665929134597	26.539468353641432
21	27.161955810402315	21.878992801271462	21.72395524946243	29.235096138863785
22	29.168874068995603	22.909719841690297	20.545202405808844	27.376203683505253
23	28.564305525257875	21.868085636822588	21.995855277509428	27.571753560410112
24	28.066471376484152	21.16924802891957	22.796752781326934	27.967527813269342
25	28.1038673688803	21.660070429119013	23.121630465268474	27.114431736732215
26	28.18567110224687	23.01255882077971	22.031693103555735	26.770076973417684
27	26.699180404499984	21.617220854498427	24.815357287544018	26.86824145345757
28	25.88815481940852	22.944778584561686	21.50425379413506	29.662812801894727
29	27.623173049954815	23.7261990090062	21.99195986163483	26.65866807940416
30	27.05444233226339	20.925394995169686	23.92953971766026	28.090622954906664
31	28.29084733086104	22.647947894917262	22.675994889214373	26.38520988500732
32	25.976970301349372	21.59930194147527	22.75000779083175	29.673719966343608
33	25.782978590794354	21.437252641091963	25.103618062264328	27.676150705849356
34	26.273800990993802	21.779270154881736	23.896039141138708	28.05088971298576
35	25.47835706940073	24.873788525663	24.23961482127832	25.40823958365795
36	26.411698712954596	22.83570694007292	24.716413724329207	26.036180622643272
37	27.32478419396055	23.08968805509676	23.554800710523853	26.03072704041883
38	26.6485399981302	22.229580229985356	24.17650908410982	26.945370687774627
39	26.001121879771883	21.309483000405123	25.301505188693945	27.387889931129045
40	25.786874006668953	22.574714076474805	26.08136744678862	25.557044470067623
41	23.305494094549534	21.41699647854405	27.044314250989437	28.233195175916983
42	21.34765807597619	22.37137336782075	28.353173984854624	27.927794571348436
43	21.07108354887968	23.19252703418617	27.696406868397272	28.03998254853688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	6.0
7	9.0
8	10.5
9	12.0
10	14.5
11	17.0
12	17.0
13	50.0
14	83.0
15	147.5
16	212.0
17	194.0
18	176.0
19	176.0
20	204.0
21	232.0
22	164.0
23	96.0
24	91.0
25	86.0
26	86.0
27	92.0
28	98.0
29	148.5
30	199.0
31	295.5
32	392.0
33	392.0
34	650.5
35	909.0
36	1074.0
37	1239.0
38	1804.0
39	2369.0
40	2369.0
41	2952.0
42	3535.0
43	4568.5
44	5602.0
45	8328.0
46	11054.0
47	11054.0
48	12214.5
49	13375.0
50	15579.0
51	17783.0
52	17415.5
53	17048.0
54	17048.0
55	15083.0
56	13118.0
57	12207.0
58	11296.0
59	10310.0
60	9324.0
61	9324.0
62	8252.0
63	7180.0
64	5604.0
65	4028.0
66	3312.0
67	2596.0
68	2596.0
69	2148.0
70	1700.0
71	1448.5
72	1197.0
73	1000.0
74	803.0
75	803.0
76	711.0
77	619.0
78	594.5
79	570.0
80	539.0
81	508.0
82	508.0
83	468.0
84	428.0
85	301.0
86	174.0
87	184.0
88	194.0
89	194.0
90	121.0
91	48.0
92	43.5
93	39.0
94	21.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	128356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.4505282183926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08966091117121	28.87048521300134
2	9.342625338048679	6.997725077129234
3	3.7278968171416684	4.1883511483686
4	2.0324526731849386	3.0446570475864005
5	1.316829623465779	2.465798248621023
6	1.0131058872477636	2.2764810371155226
7	0.7364260453505305	1.9305681074511514
8	0.545038485541918	1.6329583346318053
9	0.43270230913251506	1.4584437034497804
>10	3.2015810276679844	23.466764311757924
>50	0.36405242354899103	9.212658543426096
>100	0.18930726024547534	12.680357754993924
>500	0.008321198252548368	1.7747514724672004
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	643	0.5009504814734022	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	578	0.4503100751036181	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	552	0.43005391255570447	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	505	0.39343700333447595	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	465	0.3622736763376858	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	443	0.3451338464894512	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	437	0.34045934743993267	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	401	0.3124123531428215	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	400	0.31163326996790175	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	368	0.2867026083704696	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	365	0.28436535884571035	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	358	0.2789117766212721	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	276	0.21502695627785223	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	273	0.21268970675309296	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	253	0.19710804325469788	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	250	0.1947707937299386	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	247	0.19243354420517936	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	244	0.19009629468042008	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	241	0.18775904515566083	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	239	0.1862008788058213	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	231	0.17996821340646327	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	226	0.1760727975318645	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	214	0.16672379943282745	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	214	0.16672379943282745	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	206	0.1604911340334694	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	204	0.1589329676836299	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	201	0.15659571815887066	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	200	0.15581663498395087	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	195	0.15192121910935213	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	195	0.15192121910935213	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	193	0.1503630527595126	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	190	0.14802580323475334	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	190	0.14802580323475334	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	188	0.14646763688491382	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	186	0.14490947053507433	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	181	0.14101405466047554	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	181	0.14101405466047554	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	172	0.13400230608619776	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	171	0.133223222911278	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	167	0.130106890211599	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	165	0.12854872386175947	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	165	0.12854872386175947	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	164	0.12776964068683974	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	163	0.12699055751191995	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	163	0.12699055751191995	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	159	0.12387422481224095	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	158	0.1230951416373212	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	157	0.12231605846240144	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	156	0.1215369752874817	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	156	0.1215369752874817	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	154	0.11997880893764219	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	151	0.11764155941288292	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	150	0.11686247623796317	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	150	0.11686247623796317	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	148	0.11530430988812367	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	148	0.11530430988812367	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	147	0.11452522671320389	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	147	0.11452522671320389	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	144	0.11218797718844464	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	143	0.11140889401352488	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	143	0.11140889401352488	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	135	0.10517622861416684	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	134	0.10439714543924711	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	133	0.10361806226432733	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	133	0.10361806226432733	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	129	0.10050172956464831	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.790831749197544E-4	0.0	0.0	0.0	0.0
8	0.0023372495247592634	0.0	0.0	0.0	0.0
9	0.0023372495247592634	0.0	0.0	0.0	0.0
10	0.0023372495247592634	0.0	0.0	0.0	0.0
11	0.0023372495247592634	0.0	0.0	0.0	0.0
12	0.0023372495247592634	0.0	0.0	0.0	0.0
13	0.0023372495247592634	0.0	0.0	0.0	0.0
14	0.0023372495247592634	0.0	0.0	0.0	0.0
15	0.0023372495247592634	0.0	0.0	0.0	0.0
16	0.0023372495247592634	0.0	0.0	0.0	0.0
17	0.0023372495247592634	0.0	0.0	0.0	0.0
18	0.0023372495247592634	0.0	0.0	0.0	0.0
19	0.0023372495247592634	0.0	0.0	0.0015581663498395089	0.0
20	0.0023372495247592634	0.0	0.0	0.0023372495247592634	0.0
21	0.0023372495247592634	0.0	0.0	0.005453582224438281	0.0
22	0.0023372495247592634	0.0	0.0	0.007790831749197544	0.0
23	0.0023372495247592634	0.0	0.0	0.012465330798716071	0.0
24	0.0023372495247592634	0.0	0.0	0.015581663498395088	0.0
25	0.0023372495247592634	0.0	0.0	0.017139829848234596	0.0
26	0.0023372495247592634	0.0	0.0	0.021814328897753123	0.0
27	0.0023372495247592634	0.0	0.0	0.04051232509582723	0.0
28	0.0031163326996790177	0.0	0.0	0.11374614353828415	0.0
29	0.0031163326996790177	0.0	0.0	0.2204805385022905	0.0
30	0.003895415874598772	0.0	0.0	0.393437003334476	0.0
31	0.003895415874598772	0.0	0.0	0.7448035152232853	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGAACC	20	0.0018358518	37.0	4
GAGCAGA	20	0.0018358518	37.0	1
AAAGACT	30	3.581846E-4	30.833334	36
AGAACCC	25	0.005478395	29.6	5
GATATGC	70	6.4756023E-9	26.42857	34
ATATGCC	65	9.256655E-8	25.615385	35
CTTATTG	80	9.586074E-10	25.4375	28
TATGCCC	60	1.3220069E-6	24.666668	36
GGTATCA	285	0.0	24.666666	1
TTATTGA	85	1.9354047E-9	23.941177	29
CTCTATG	135	0.0	23.296297	1
GTACTGC	40	0.001921906	23.125	6
GTGGTCA	40	0.001921906	23.125	29
CAGTCGG	90	3.748937E-9	22.611113	10
GTCGGTG	90	3.748937E-9	22.611113	12
AGTCGGT	90	3.748937E-9	22.611113	11
GTCAATG	115	5.456968E-12	22.521738	31
AATGAGC	100	5.2204996E-10	22.2	34
TCAATGA	110	7.2759576E-11	21.863636	32
CGTCAAT	120	9.094947E-12	21.583334	30
>>END_MODULE