FastQCFastQC Report
Fri 10 Feb 2017
ERR1631806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268995
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54590.430182940043105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53670.4229331084834849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43450.3423969361581409No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17050.13435829140382743No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15900.1252960019543024No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15330.12080425848801611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.11615490998782502No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14500.11426364958096762No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13670.10772304067391913No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13380.10543776768229979No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13360.10528016264839499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.027.0073011
AAACCGT555.1434984E-420.1818186
TACGAGG609.237539E-418.57
GTACTCG500.00703527518.56
GCAGTCG3750.017.7600029
TAATACT2300.017.695654
CTTATAC13950.017.63799337
CAGTCGG3800.017.52631610
GTATACT752.0674805E-417.2666664
TATACAC4400.017.23863637
GTATCAA32250.017.2093032
TCTAATA2550.016.6862742
AGTCGGT4000.016.65000211
ATTAGAG2800.016.5178573
CTCTATG6250.016.281
TATTAGA2750.016.1454542
GTATTAG4300.015.9186041
GACAGTT1059.344549E-615.8571447
TAGAGGT2450.015.85714319
ACGTGAG700.002592692915.85714211
Produced by FastQC (version 0.11.5)