##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631806.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1268995
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61424591901466	34.0	31.0	34.0	31.0	34.0
2	32.780349016347586	34.0	31.0	34.0	31.0	34.0
3	32.86357787067719	34.0	31.0	34.0	31.0	34.0
4	36.28940303153283	37.0	37.0	37.0	35.0	37.0
5	36.205749431636846	37.0	35.0	37.0	35.0	37.0
6	36.261420257763035	37.0	37.0	37.0	35.0	37.0
7	36.244909554411166	37.0	37.0	37.0	35.0	37.0
8	36.23283937288957	37.0	37.0	37.0	35.0	37.0
9	38.04216565077088	39.0	38.0	39.0	35.0	39.0
10	37.976246557315044	39.0	38.0	39.0	35.0	39.0
11	38.048485612630465	39.0	38.0	39.0	35.0	39.0
12	37.9937218034744	39.0	38.0	39.0	35.0	39.0
13	38.04137447350068	39.0	38.0	39.0	35.0	39.0
14	39.4443941859503	40.0	39.0	41.0	37.0	41.0
15	39.43196545297657	40.0	39.0	41.0	37.0	41.0
16	39.390490112254184	40.0	39.0	41.0	37.0	41.0
17	39.36600223011123	40.0	39.0	41.0	37.0	41.0
18	39.39058703935004	40.0	39.0	41.0	37.0	41.0
19	39.421499690700124	40.0	39.0	41.0	37.0	41.0
20	39.40402208046525	40.0	39.0	41.0	37.0	41.0
21	39.37525758572729	40.0	39.0	41.0	37.0	41.0
22	39.34748285060225	40.0	39.0	41.0	36.0	41.0
23	39.28225327916974	40.0	39.0	41.0	36.0	41.0
24	39.29688848261814	40.0	39.0	41.0	36.0	41.0
25	39.23258641681015	40.0	39.0	41.0	36.0	41.0
26	39.16409048104997	40.0	39.0	41.0	36.0	41.0
27	39.071788304918456	40.0	39.0	41.0	35.0	41.0
28	39.05125788517685	40.0	39.0	41.0	35.0	41.0
29	39.01598666661413	40.0	39.0	41.0	35.0	41.0
30	38.951792560254376	40.0	38.0	41.0	35.0	41.0
31	38.84880161072345	40.0	38.0	41.0	35.0	41.0
32	38.77618430332665	40.0	38.0	41.0	35.0	41.0
33	38.70829908707284	40.0	38.0	41.0	35.0	41.0
34	38.65881110642674	40.0	38.0	41.0	35.0	41.0
35	38.57532299181636	40.0	38.0	41.0	35.0	41.0
36	38.513983112620615	40.0	38.0	41.0	35.0	41.0
37	38.4563091265135	40.0	38.0	41.0	35.0	41.0
38	38.40820491806508	40.0	38.0	41.0	35.0	41.0
39	38.32796819530416	40.0	38.0	41.0	34.0	41.0
40	37.99308665518777	40.0	37.0	41.0	34.0	41.0
41	38.039398106375515	40.0	37.0	41.0	34.0	41.0
42	37.990320686842736	40.0	37.0	41.0	34.0	41.0
43	37.51476246951328	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	2.0
12	2.0
13	1.0
14	0.0
15	2.0
16	1.0
17	8.0
18	11.0
19	21.0
20	66.0
21	89.0
22	178.0
23	381.0
24	701.0
25	1160.0
26	1968.0
27	3028.0
28	4616.0
29	6711.0
30	9534.0
31	12663.0
32	16747.0
33	22608.0
34	32578.0
35	47694.0
36	73640.0
37	138379.0
38	300249.0
39	595953.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.57904877481787	17.823001666673232	13.154267747311849	26.443681811197052
2	19.489438492665457	20.35618737662481	34.349623126962676	25.80475100374706
3	20.455872560569585	21.70418323161242	29.935736547425325	27.90420766039267
4	14.957032927631708	16.661216159244127	34.54891469233527	33.83283622078889
5	16.0145627051328	33.979960519939006	33.86813974838356	16.13733702654463
6	32.860964779215045	34.693123298358145	15.950023443748792	16.49588847867801
7	29.06733281060997	29.26646677094866	21.301187159917887	20.365013258523476
8	26.633911087120122	32.453240556503374	20.321435466648804	20.591412889727696
9	27.443134133704227	14.064042805527208	19.4777757201565	39.01504734061206
10	18.583839967848572	25.060776441199533	31.294055532133697	25.061328058818198
11	36.15554040796063	21.21324355099902	20.71276876583438	21.91844727520597
12	22.18527259760677	25.29608075681937	28.057399753348122	24.461246892225738
13	32.16505975200848	19.297869573954195	23.210020528055665	25.327050145981662
14	23.142171560959657	21.21726247936359	25.1783497964925	30.46221616318425
15	27.55810700593777	25.792457811102487	22.327905153290594	24.321530029669148
16	24.31136450498229	25.540132151820927	24.42807103258878	25.720432310608
17	24.628859845783474	25.34210142671957	24.58323318846804	25.445805539028914
18	24.44517117876745	23.6098644990721	26.181190627228634	25.763773694931817
19	25.773702812067818	23.98488567724853	26.468268196486193	23.773143314197455
20	26.654399741527747	23.043195599667456	26.203728147077015	24.098676511727785
21	25.091430620294012	24.41451699967297	25.32712894849862	25.166923431534403
22	25.547224378346645	24.773541266908065	24.8653461991576	24.81388815558769
23	25.12854660577859	24.31593505096553	25.765349745270864	24.79016859798502
24	25.356049472220143	24.19331833458761	25.445569131478056	25.00506306171419
25	25.241076599986606	24.033979645309874	26.00601263204347	24.718931122660056
26	25.08804211206506	24.734612823533585	25.573308011457886	24.604037052943468
27	25.022005602858954	23.986225320036723	26.13461833970977	24.85715073739455
28	24.20001654852856	24.598284469205947	25.614206517756177	25.587492464509314
29	25.005535876815905	25.07480328921706	25.589620132467033	24.330040701500007
30	24.532326762516796	24.31806271892324	26.44636109677343	24.703249421786534
31	25.04139102202924	24.472358047116025	25.86424690404612	24.622004026808618
32	23.41734995015741	24.414911012257733	26.054554982486138	26.11318405509872
33	23.760771319035932	23.981733576570434	27.190572066871816	25.06692303752182
34	24.28307440139638	23.740440269662212	26.763147214922046	25.21333811401936
35	24.017588721783774	24.725156521499297	26.723273141344134	24.533981615372795
36	24.141151068365126	23.874877363582993	26.99860913557579	24.985362432476094
37	24.195524805062274	23.472275304473225	26.815235678627573	25.516964211836925
38	23.787012557181075	22.95643402850287	27.808620207329422	25.447933206986633
39	23.388981044054546	22.558244910342438	28.506258889908942	25.54651515569407
40	23.086615786508222	23.3495009830614	28.713509509493733	24.85037372093665
41	21.908124145485207	22.679837193999976	29.681519627736908	25.730519032777906
42	21.072344650688144	23.302849893025584	29.690818324737293	25.93398713154898
43	20.76682729246372	23.4961524671098	29.576239465088516	26.160780775337965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	116.0
2	100.0
3	255.5
4	411.0
5	411.0
6	521.5
7	632.0
8	684.0
9	736.0
10	1189.5
11	1643.0
12	1643.0
13	3071.0
14	4499.0
15	7663.5
16	10828.0
17	10644.5
18	10461.0
19	10461.0
20	11823.5
21	13186.0
22	11412.0
23	9638.0
24	10065.5
25	10493.0
26	10493.0
27	11598.0
28	12703.0
29	15459.5
30	18216.0
31	21208.5
32	24201.0
33	24201.0
34	28404.5
35	32608.0
36	36034.5
37	39461.0
38	45087.0
39	50713.0
40	50713.0
41	56514.0
42	62315.0
43	67960.5
44	73606.0
45	84258.0
46	94910.0
47	94910.0
48	100958.0
49	107006.0
50	116936.5
51	126867.0
52	125047.0
53	123227.0
54	123227.0
55	111915.5
56	100604.0
57	92422.5
58	84241.0
59	76589.5
60	68938.0
61	68938.0
62	62819.0
63	56700.0
64	47823.5
65	38947.0
66	33760.0
67	28573.0
68	28573.0
69	24730.0
70	20887.0
71	17964.5
72	15042.0
73	12403.5
74	9765.0
75	9765.0
76	8061.0
77	6357.0
78	5218.5
79	4080.0
80	3333.5
81	2587.0
82	2587.0
83	2070.0
84	1553.0
85	1310.0
86	1067.0
87	835.0
88	603.0
89	603.0
90	482.5
91	362.0
92	219.5
93	77.0
94	47.5
95	18.0
96	18.0
97	9.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1268995.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.833542589592184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09550662421948	44.2737314534437
2	6.405173957616955	6.5119536633653645
3	2.0367265091388207	3.1060207123697894
4	1.0492878135341217	2.1335606703210734
5	0.6064895731151198	1.5415006772545512
6	0.4166856899646387	1.2708965860374641
7	0.2870928519130878	1.0215762700424003
8	0.23380372665319504	0.9508057357144448
9	0.17837223084241519	0.8160563153995634
>10	1.3604085487403799	14.08867334293772
>50	0.18841550696876386	6.6484816960284245
>100	0.13240207668641085	12.658489663769949
>500	0.007148467224239663	2.3872611768894205
>1k	0.002175620459551202	1.7357806035668844
>5k	3.1080292279302884E-4	0.8552114328591949
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5459	0.430182940043105	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5367	0.4229331084834849	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4345	0.3423969361581409	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1705	0.13435829140382743	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1590	0.1252960019543024	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1533	0.12080425848801611	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1474	0.11615490998782502	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1450	0.11426364958096762	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1367	0.10772304067391913	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1338	0.10543776768229979	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1336	0.10528016264839499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.880251695239147E-5	0.0	0.0	0.0	0.0
8	1.5760503390478293E-4	0.0	0.0	0.0	0.0
9	1.5760503390478293E-4	0.0	7.880251695239147E-5	0.0	0.0
10	1.5760503390478293E-4	0.0	7.880251695239147E-5	0.0	0.0
11	1.5760503390478293E-4	0.0	7.880251695239147E-5	0.0	7.880251695239147E-5
12	1.5760503390478293E-4	0.0	7.880251695239147E-5	7.880251695239147E-5	7.880251695239147E-5
13	2.3640755085717438E-4	0.0	7.880251695239147E-5	1.5760503390478293E-4	7.880251695239147E-5
14	2.3640755085717438E-4	0.0	7.880251695239147E-5	2.3640755085717438E-4	7.880251695239147E-5
15	2.3640755085717438E-4	0.0	7.880251695239147E-5	4.7281510171434877E-4	7.880251695239147E-5
16	2.3640755085717438E-4	0.0	7.880251695239147E-5	0.001024432720381089	7.880251695239147E-5
17	3.940125847619573E-4	0.0	7.880251695239147E-5	0.0015760503390478292	7.880251695239147E-5
18	3.940125847619573E-4	0.0	7.880251695239147E-5	0.0021276679577145693	7.880251695239147E-5
19	3.940125847619573E-4	0.0	7.880251695239147E-5	0.002758088093333701	7.880251695239147E-5
20	3.940125847619573E-4	0.0	7.880251695239147E-5	0.003624915779810007	7.880251695239147E-5
21	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.005200966118857836	7.880251695239147E-5
22	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.007171029042667623	7.880251695239147E-5
23	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.009456302034286976	7.880251695239147E-5
24	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.01237199516152546	7.880251695239147E-5
25	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.014263255568382854	7.880251695239147E-5
26	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.018833801551621558	7.880251695239147E-5
27	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.04468102711200596	7.880251695239147E-5
28	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.14617866894668616	7.880251695239147E-5
29	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.3061477783600408	7.880251695239147E-5
30	3.940125847619573E-4	0.0	1.5760503390478293E-4	0.49290974353720857	7.880251695239147E-5
31	3.940125847619573E-4	0.0	1.5760503390478293E-4	1.0546928868908072	7.880251695239147E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2055	0.0	27.007301	1
AAACCGT	55	5.1434984E-4	20.181818	6
TACGAGG	60	9.237539E-4	18.5	7
GTACTCG	50	0.007035275	18.5	6
GCAGTCG	375	0.0	17.760002	9
TAATACT	230	0.0	17.69565	4
CTTATAC	1395	0.0	17.637993	37
CAGTCGG	380	0.0	17.526316	10
GTATACT	75	2.0674805E-4	17.266666	4
TATACAC	440	0.0	17.238636	37
GTATCAA	3225	0.0	17.209303	2
TCTAATA	255	0.0	16.686274	2
AGTCGGT	400	0.0	16.650002	11
ATTAGAG	280	0.0	16.517857	3
CTCTATG	625	0.0	16.28	1
TATTAGA	275	0.0	16.145454	2
GTATTAG	430	0.0	15.918604	1
GACAGTT	105	9.344549E-6	15.857144	7
TAGAGGT	245	0.0	15.857143	19
ACGTGAG	70	0.0025926929	15.857142	11
>>END_MODULE