FastQCFastQC Report
Fri 10 Feb 2017
ERR1631805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724536
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22880.31578831141585784No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT16890.23311471065619926No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12510.17266222796382788No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12200.1683836275906235No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG12190.16824560822374596No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12060.16645135645433767No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11280.15568584583788797No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT10410.14367816091954022No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT10110.13953757991321342No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC9850.13594907637439685No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG9630.13291265030309052No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9520.13139443726743735No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9330.1287720692967637No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT9230.12739187562798812No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8870.12242317842039595No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8760.12090496538474278No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG8150.11248578400521161No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG8050.111105590336436No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT8030.1108295516026809No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7950.1097253966676604No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7950.1097253966676604No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC7780.10737906743074188No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7550.10420462199255799No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG7530.10392858325880287No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG7440.10268640895690483No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG7400.10213433148939459No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7320.10103017655437412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.028.1904751
TAAGGAC358.866218E-426.428573
ACTGCGC451.3225524E-424.6666688
AACGACC751.3718818E-824.66666637
ATACGGC601.3362678E-624.66666629
TTAGAAT400.001930501823.1253
TCAAGCC1200.023.12499831
CCAGGAC6000.022.5083333
ACAGGCT6000.020.9666678
GCGGTAA4850.020.59793923
TCGTTCA450.003824462920.55555710
TATTGAA450.003824462920.5555572
TTAAGCT450.003824462920.5555574
GACTGCG450.003824462920.5555577
TTCGAAC450.003824462920.5555572
GACAGGC6350.020.3937027
TCCAGGA6850.019.9854012
GTATTGC656.897631E-519.9230771
CGGTAAT5200.019.21153824
CGTGCCA5300.019.19811410
Produced by FastQC (version 0.11.5)