FastQCFastQC Report
Fri 10 Feb 2017
ERR1631804.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631804.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071917
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.36989804247903524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39380.36737919073958153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30520.2847235373634339No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14890.13891000889061372No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14690.1370441927873147No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13980.1304205456206031No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12490.11652021565102522No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12480.11642692484586027No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12250.11428123632706638No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12120.11306845585992199No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11700.109150242042994No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11050.10308633970727211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.027.575471
TATAGAC400.001931102623.1252
TATACCA705.101396E-621.1428575
TATAATC450.00382564320.5555553
TCTATAC555.142784E-420.1818183
TTCGCTA656.901577E-519.92307726
CTCTATG4850.018.3092781
ATACACA1950.018.02564237
TAATACT1552.0008883E-1117.9032254
CTTATAC10250.017.68780537
TCTAATA1900.017.5263162
TCTATGG5100.017.4117642
GTATCAA25350.017.1499022
GGATCGT650.001579840217.0769236
TCTACGG650.001579840217.0769233
TATACAC3000.016.6537
TATAACT904.4455075E-516.4444454
TACCCGA803.382299E-416.187511
TTTACAC803.382299E-416.18753
TATTAGA2400.016.18752
Produced by FastQC (version 0.11.5)