##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631804.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071917
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61905912491359	34.0	31.0	34.0	31.0	34.0
2	32.78953221191566	34.0	31.0	34.0	31.0	34.0
3	32.874047151038745	34.0	31.0	34.0	31.0	34.0
4	36.29999430926088	37.0	37.0	37.0	35.0	37.0
5	36.21573685276005	37.0	35.0	37.0	35.0	37.0
6	36.269827794502746	37.0	37.0	37.0	35.0	37.0
7	36.257735440337264	37.0	37.0	37.0	35.0	37.0
8	36.24721223751466	37.0	37.0	37.0	35.0	37.0
9	38.06185366964046	39.0	38.0	39.0	37.0	39.0
10	38.00057093972761	39.0	38.0	39.0	35.0	39.0
11	38.07034779745074	39.0	38.0	39.0	37.0	39.0
12	38.01451511637561	39.0	38.0	39.0	35.0	39.0
13	38.05754176862574	39.0	38.0	39.0	37.0	39.0
14	39.462507824766284	40.0	39.0	41.0	37.0	41.0
15	39.46442495081242	40.0	39.0	41.0	37.0	41.0
16	39.42291427414622	40.0	39.0	41.0	37.0	41.0
17	39.388412535672074	40.0	39.0	41.0	37.0	41.0
18	39.40613592283731	40.0	39.0	41.0	37.0	41.0
19	39.43996130297402	40.0	39.0	41.0	37.0	41.0
20	39.43154274071593	40.0	39.0	41.0	37.0	41.0
21	39.40580660629508	40.0	39.0	41.0	37.0	41.0
22	39.37962640764164	40.0	39.0	41.0	36.0	41.0
23	39.318137505049364	40.0	39.0	41.0	36.0	41.0
24	39.33188763682263	40.0	39.0	41.0	36.0	41.0
25	39.28002261369117	40.0	39.0	41.0	36.0	41.0
26	39.215048366617935	40.0	39.0	41.0	36.0	41.0
27	39.11808003791338	40.0	39.0	41.0	36.0	41.0
28	39.09627984256244	40.0	39.0	41.0	35.0	41.0
29	39.065217736074715	40.0	39.0	41.0	35.0	41.0
30	39.00385197734526	40.0	39.0	41.0	35.0	41.0
31	38.91108826522949	40.0	38.0	41.0	35.0	41.0
32	38.84882131732214	40.0	38.0	41.0	35.0	41.0
33	38.7796275271313	40.0	38.0	41.0	35.0	41.0
34	38.73503079063025	40.0	38.0	41.0	35.0	41.0
35	38.65802482841489	40.0	38.0	41.0	35.0	41.0
36	38.60146354615143	40.0	38.0	41.0	35.0	41.0
37	38.54754612530635	40.0	38.0	41.0	35.0	41.0
38	38.49753852210572	40.0	38.0	41.0	35.0	41.0
39	38.42703492901036	40.0	38.0	41.0	35.0	41.0
40	38.09911215140725	40.0	38.0	41.0	34.0	41.0
41	38.15194926472852	40.0	38.0	41.0	34.0	41.0
42	38.11530277064362	40.0	37.0	41.0	34.0	41.0
43	37.646152640549595	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	0.0
15	1.0
16	1.0
17	7.0
18	9.0
19	19.0
20	30.0
21	66.0
22	157.0
23	295.0
24	561.0
25	863.0
26	1509.0
27	2299.0
28	3574.0
29	5299.0
30	7308.0
31	10166.0
32	13460.0
33	18433.0
34	26593.0
35	39601.0
36	61526.0
37	114969.0
38	253871.0
39	511296.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.53286401838948	17.54221642160727	13.249719894357492	26.675199665645756
2	19.421466400850065	20.40904286432625	34.565829257302575	25.60366147752111
3	20.607379116106937	21.33747295732785	30.072570917337814	27.982577009227395
4	15.204348844173568	16.578335822642984	34.21458937585653	34.002725957326916
5	15.975677221277392	34.056088297881274	33.81194626076459	16.156288220076743
6	33.016548855928214	34.350980533007686	16.11085559796141	16.521615013102693
7	29.051129891586758	29.200954924681668	21.012167919717665	20.735747264013913
8	26.373217329326803	32.59179582001218	20.449437782962672	20.585549067698338
9	27.392326084948742	14.219757686462664	19.248691829684574	39.13922439890402
10	18.59770859124354	25.190756373861035	31.133100790452993	25.078434244442434
11	36.57801863390542	21.114321351373288	20.55522955601973	21.75243045870156
12	21.979033824447228	25.115937148118743	27.889006331646947	25.016022695787083
13	32.482925450384684	19.05529999057763	23.09898994045248	25.36278461858521
14	23.149273684436388	21.16600445743467	24.81498101065661	30.869740847472332
15	27.644397840504443	25.93475054505153	22.055625575487657	24.365226038956376
16	24.436220341686905	25.52287164024827	24.071453293491942	25.969454724572895
17	24.985236730082647	25.269587104225423	24.06053826928764	25.684637896404293
18	24.602184684075354	23.48838576121099	25.863849533126164	26.045580021587494
19	26.130941108313422	23.920322189124718	26.080097619498527	23.868639083063332
20	26.96766633983788	22.919311849704783	25.863103206684844	24.249918603772493
21	25.285073377882807	24.366625401033847	24.78531453461415	25.5629866864692
22	25.652639150232716	24.401422871360374	24.47512260744069	25.47081537096622
23	25.271079757108062	24.251691129070625	25.266415216849815	25.2108138969715
24	25.977384443011914	23.849234595589024	25.13916655860482	25.034214402794248
25	25.67484236186197	23.75361152029495	25.59181354526516	24.979732572577916
26	25.61709535346487	24.37604777235551	25.053712181073724	24.9531446931059
27	25.30027977912469	23.633266381632158	25.85806550320594	25.208388336037213
28	24.370263742435284	24.508987169715564	25.220516140708654	25.900232947140495
29	25.2033506325583	25.081419550207713	25.012104481970155	24.70312533526383
30	24.764230812646876	24.38780241380629	25.88334731140564	24.96461946214119
31	25.46605753990281	24.145619483598075	25.477439018132937	24.910883958366178
32	23.67785938650101	24.255982506108214	25.66103532269756	26.40512278469322
33	24.112594538569684	23.8759157658662	26.67482650242509	25.33666319313902
34	24.448534727968678	23.809492712588753	26.162939854485	25.579032704957566
35	24.371196650486933	24.702845462848337	26.176000567208096	24.749957319456637
36	24.47157755684442	23.88421864752588	26.594316537567742	25.049887258061958
37	24.750517064287628	23.267286552970052	26.327504834795977	25.654691547946346
38	24.049529954278174	23.08294392196411	27.34194905015967	25.52557707359805
39	23.628788422984243	22.45640287447629	28.135294057282422	25.779514645257052
40	23.520477798187734	23.025010331956672	28.474499424862188	24.98001244499341
41	22.156006481845143	22.804097705326065	29.282024634370014	25.757871178458778
42	21.619491061341503	22.93797001073777	29.29182016891233	26.150718759008395
43	20.921675838707664	23.58979286642529	29.101973380401652	26.386557914465392
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	56.0
2	58.0
3	147.0
4	236.0
5	236.0
6	304.5
7	373.0
8	413.5
9	454.0
10	714.0
11	974.0
12	974.0
13	1917.5
14	2861.0
15	5420.0
16	7979.0
17	7788.5
18	7598.0
19	7598.0
20	8497.0
21	9396.0
22	8287.5
23	7179.0
24	7505.5
25	7832.0
26	7832.0
27	8626.0
28	9420.0
29	12564.0
30	15708.0
31	17307.5
32	18907.0
33	18907.0
34	22041.0
35	25175.0
36	27114.5
37	29054.0
38	35563.5
39	42073.0
40	42073.0
41	47878.0
42	53683.0
43	56681.5
44	59680.0
45	70763.0
46	81846.0
47	81846.0
48	88085.0
49	94324.0
50	103572.0
51	112820.0
52	112413.0
53	112006.0
54	112006.0
55	98717.0
56	85428.0
57	77979.0
58	70530.0
59	64159.5
60	57789.0
61	57789.0
62	52558.0
63	47327.0
64	40119.5
65	32912.0
66	28670.5
67	24429.0
68	24429.0
69	21158.5
70	17888.0
71	15518.0
72	13148.0
73	10768.5
74	8389.0
75	8389.0
76	6902.0
77	5415.0
78	4488.0
79	3561.0
80	2888.5
81	2216.0
82	2216.0
83	1850.5
84	1485.0
85	1178.5
86	872.0
87	684.0
88	496.0
89	496.0
90	377.0
91	258.0
92	163.0
93	68.0
94	41.5
95	15.0
96	15.0
97	7.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1071917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.27339889545399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.21579594750955	40.757137129156845
2	6.698217144432994	6.332949819142994
3	2.1593767575573755	3.062432364767455
4	1.085443143323139	2.0525034677060074
5	0.6881612255860792	1.626586006075761
6	0.4574443056913599	1.2974968281240988
7	0.32586307145193344	1.0783258473431554
8	0.23954090118971888	0.9059130058974488
9	0.20596096150298251	0.8762827221019561
>10	1.5245617909681395	14.445964287539656
>50	0.22142811779322075	7.372007261753656
>100	0.16571829278512795	14.878354994967513
>500	0.009911381117818493	3.104577004540403
>1k	0.002576959090632808	2.209469260882995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3965	0.36989804247903524	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.36737919073958153	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3052	0.2847235373634339	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1489	0.13891000889061372	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1469	0.1370441927873147	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1398	0.1304205456206031	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1249	0.11652021565102522	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1248	0.11642692484586027	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1225	0.11428123632706638	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1212	0.11306845585992199	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1170	0.109150242042994	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1105	0.10308633970727211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	9.329080516495214E-5	0.0	0.0	0.0	0.0
9	9.329080516495214E-5	0.0	0.0	0.0	0.0
10	1.8658161032990427E-4	0.0	0.0	0.0	0.0
11	1.8658161032990427E-4	0.0	0.0	0.0	0.0
12	2.798724154948564E-4	0.0	0.0	1.8658161032990427E-4	0.0
13	3.7316322065980855E-4	0.0	0.0	1.8658161032990427E-4	0.0
14	3.7316322065980855E-4	0.0	0.0	2.798724154948564E-4	0.0
15	3.7316322065980855E-4	0.0	0.0	5.597448309897128E-4	0.0
16	3.7316322065980855E-4	0.0	0.0	9.329080516495213E-4	0.0
17	3.7316322065980855E-4	0.0	0.0	0.0011194896619794257	0.0
18	3.7316322065980855E-4	0.0	0.0	0.0013060712723093298	0.0
19	3.7316322065980855E-4	0.0	0.0	0.0018658161032990427	0.0
20	5.597448309897128E-4	0.0	0.0	0.002705433349783612	0.0
21	5.597448309897128E-4	0.0	0.0	0.004198086232422846	0.0
22	5.597448309897128E-4	0.0	0.0	0.007276682802866267	0.0
23	5.597448309897128E-4	0.0	0.0	0.008955917295835406	0.0
24	5.597448309897128E-4	0.0	0.0	0.011381478230124161	0.0
25	5.597448309897128E-4	0.0	0.0	0.012500967892103587	0.0
26	5.597448309897128E-4	0.0	0.0	0.01613930929353672	0.0
27	5.597448309897128E-4	0.0	0.0	0.03619683240400143	0.0
28	5.597448309897128E-4	0.0	0.0	0.12193108235059244	0.0
29	5.597448309897128E-4	0.0	0.0	0.2508589750885563	0.0
30	6.530356361546649E-4	0.0	0.0	0.4126252312445833	0.0
31	7.463264413196171E-4	0.0	0.0	0.9039879020483862	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1590	0.0	27.57547	1
TATAGAC	40	0.0019311026	23.125	2
TATACCA	70	5.101396E-6	21.142857	5
TATAATC	45	0.003825643	20.555555	3
TCTATAC	55	5.142784E-4	20.181818	3
TTCGCTA	65	6.901577E-5	19.923077	26
CTCTATG	485	0.0	18.309278	1
ATACACA	195	0.0	18.025642	37
TAATACT	155	2.0008883E-11	17.903225	4
CTTATAC	1025	0.0	17.687805	37
TCTAATA	190	0.0	17.526316	2
TCTATGG	510	0.0	17.411764	2
GTATCAA	2535	0.0	17.149902	2
GGATCGT	65	0.0015798402	17.076923	6
TCTACGG	65	0.0015798402	17.076923	3
TATACAC	300	0.0	16.65	37
TATAACT	90	4.4455075E-5	16.444445	4
TACCCGA	80	3.382299E-4	16.1875	11
TTTACAC	80	3.382299E-4	16.1875	3
TATTAGA	240	0.0	16.1875	2
>>END_MODULE