FastQCFastQC Report
Fri 10 Feb 2017
ERR1631803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191808
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6570.34253003003003No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5510.2872664330997664No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5380.2804888221554888No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4900.2554637971304638No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4400.22939606272939606No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4270.22261845178511847No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3880.2022856189522856No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3840.20020020020020018No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3750.19550800800800802No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3460.18038872205538872No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3420.1783033033033033No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3050.15901317984651317No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2740.14285118451785117No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2670.1392017017017017No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2660.13868034701368034No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2600.13555221888555222No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2530.13190273606940273No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2480.12929596262929596No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2450.1277318985652319No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2420.12616783450116784No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2400.12512512512512514No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2360.12303970637303971No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2330.12147564230897563No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2300.11991157824491158No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2230.1162620954287621No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2210.11521938605271939No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2120.1105271938605272No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2120.1105271938605272No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2110.11000583917250584No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2100.10948448448448449No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2030.105835001668335No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2000.10427093760427093No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1960.10218551885218552No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1930.10062145478812147No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1930.10062145478812147No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1920.10010010010010009No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1920.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC200.00183811637.024
TCGCCAT303.5884054E-430.83333413
CATTGTT250.005485075529.59999817
TTATTGT250.005485075529.59999812
GGTATCA2400.026.9791681
ATACGGC358.842367E-426.42857229
GGACCGT400.001925358523.1256
TACACGG855.124275E-821.7647065
AGTGAAC603.705493E-521.58333432
TTGATAT450.00381437120.55555532
GTACACG909.375435E-820.5555554
ATATGCC450.00381437120.55555535
TATGCCC450.00381437120.55555536
GCGTACA909.375435E-820.5555552
GTAACGT1202.3464963E-1020.04166826
TAGTGAA656.8638896E-519.92307731
TAATACG2550.019.58823627
AGCGTAC951.6580088E-719.4736841
ACACGGT951.6580088E-719.4736846
CACGGTG951.6580088E-719.4736847
Produced by FastQC (version 0.11.5)