FastQCFastQC Report
Fri 10 Feb 2017
ERR1631802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC180.3447615399348784No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG170.32560812104960735No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA140.2681478643937943No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA140.2681478643937943No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA120.22984102662325223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT120.22984102662325223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120.22984102662325223No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC100.1915341888527102No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT100.1915341888527102No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA90.1723807699674392No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC90.1723807699674392No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA90.1723807699674392No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA80.15322735108216817No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG80.15322735108216817No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC80.15322735108216817No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA80.15322735108216817No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA80.15322735108216817No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA80.15322735108216817No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT70.13407393219689714No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT70.13407393219689714No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT70.13407393219689714No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA70.13407393219689714No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG70.13407393219689714No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC70.13407393219689714No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC70.13407393219689714No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT70.13407393219689714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.13407393219689714No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA70.13407393219689714No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT70.13407393219689714No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG60.11492051331162612No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA60.11492051331162612No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT60.11492051331162612RNA PCR Primer, Index 46 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCT500.006157597518.537
Produced by FastQC (version 0.11.5)