##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631802.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5221
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40049798889102	33.0	31.0	34.0	30.0	34.0
2	32.60448190001915	34.0	31.0	34.0	31.0	34.0
3	32.66443210113005	34.0	31.0	34.0	31.0	34.0
4	36.157058034859226	37.0	35.0	37.0	35.0	37.0
5	36.08542424822831	37.0	35.0	37.0	35.0	37.0
6	36.130626316797546	37.0	35.0	37.0	35.0	37.0
7	36.11281363723425	37.0	35.0	37.0	35.0	37.0
8	36.0846581114729	37.0	35.0	37.0	35.0	37.0
9	37.85730702930473	39.0	38.0	39.0	35.0	39.0
10	37.80980655046926	39.0	38.0	39.0	35.0	39.0
11	37.847155717295536	39.0	38.0	39.0	35.0	39.0
12	37.775713464853474	39.0	38.0	39.0	35.0	39.0
13	37.864776862669984	39.0	38.0	39.0	35.0	39.0
14	39.12775330396476	40.0	39.0	41.0	36.0	41.0
15	39.136180808274275	40.0	38.0	41.0	36.0	41.0
16	39.085615782417165	40.0	38.0	41.0	36.0	41.0
17	39.030453936027584	40.0	38.0	41.0	36.0	41.0
18	39.04443593181383	40.0	38.0	41.0	36.0	41.0
19	39.081018961884695	40.0	39.0	41.0	35.0	41.0
20	39.125263359509674	40.0	39.0	41.0	36.0	41.0
21	39.08121049607355	40.0	39.0	41.0	36.0	41.0
22	39.108599885079485	40.0	39.0	41.0	36.0	41.0
23	39.019536487262975	40.0	39.0	41.0	35.0	41.0
24	38.99521164527868	40.0	38.0	41.0	35.0	41.0
25	38.92798314499138	40.0	38.0	41.0	35.0	41.0
26	38.87128902509098	40.0	38.0	41.0	35.0	41.0
27	38.758666922045585	40.0	38.0	41.0	35.0	41.0
28	38.7845240375407	40.0	38.0	41.0	35.0	41.0
29	38.71863627657537	40.0	38.0	41.0	35.0	41.0
30	38.663474430185786	40.0	38.0	41.0	35.0	41.0
31	38.565025857115494	40.0	38.0	41.0	34.0	41.0
32	38.447806933537635	40.0	38.0	41.0	34.0	41.0
33	38.3673625742195	40.0	38.0	41.0	34.0	41.0
34	38.29860180042137	40.0	38.0	41.0	34.0	41.0
35	38.26010342846198	40.0	38.0	41.0	34.0	41.0
36	38.29898486879908	40.0	38.0	41.0	34.0	41.0
37	38.15897337674775	40.0	38.0	41.0	33.0	41.0
38	38.22045585136947	40.0	37.0	41.0	34.0	41.0
39	38.12775330396476	40.0	37.0	41.0	34.0	41.0
40	37.7452595288259	40.0	37.0	41.0	33.0	41.0
41	37.88910170465428	40.0	37.0	41.0	33.0	41.0
42	37.896954606397244	40.0	37.0	41.0	33.0	41.0
43	37.32771499712699	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	3.0
24	2.0
25	4.0
26	10.0
27	20.0
28	25.0
29	36.0
30	54.0
31	57.0
32	98.0
33	126.0
34	171.0
35	216.0
36	367.0
37	599.0
38	1188.0
39	2244.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.6929706952691	16.414479984677264	13.426546638574985	27.466002681478646
2	21.145374449339208	19.632254357402797	31.603141160697184	27.61923003256081
3	20.321777437272555	19.977015897337676	29.955947136563875	29.745259528825898
4	15.207814594905193	16.567707335759433	33.71001723807699	34.51446083125838
5	15.820723999233863	34.380386899061484	32.73319287492817	17.06569622677648
6	32.330971078337484	34.28461980463513	16.261252633595095	17.123156483432293
7	29.03658303007087	28.71097490902126	20.187703505075653	22.064738555832218
8	27.293621911511206	31.794675349549895	19.919555640681864	20.99214709825704
9	27.38938900593756	13.062631679754835	18.291515035433825	41.25646427887378
10	20.26431718061674	23.424631296686456	28.32790653131584	27.98314499138096
11	40.27963991572496	20.149396667305115	17.25723041562919	22.313733001340736
12	22.52442060907872	25.11013215859031	26.584945412756177	25.78050181957479
13	35.58705228883356	17.831832982187322	20.475004788354724	26.1061099406244
14	24.248228308753113	21.16452786822448	22.735108216816702	31.852135606205707
15	29.515418502202646	24.592989848687992	19.440720168550087	26.45087148055928
16	24.478069335376365	24.478069335376365	22.754261635701972	28.2895996935453
17	27.79161080252825	24.20992147098257	21.5475962459299	26.45087148055928
18	26.738172763838346	21.681670178126797	23.290557364489562	28.2895996935453
19	29.132350124497226	22.026431718061673	23.041562918981036	25.79965523846007
20	30.760390729745257	20.45585136946945	22.256272744684928	26.527485156100365
21	27.906531315839878	22.677647960160886	21.451829151503542	27.96399157249569
22	28.730128327906527	22.69680137904616	21.336908638191918	27.23616165485539
23	28.404520206856926	22.064738555832218	22.12219881248803	27.40854242482283
24	27.121241141543766	21.279448381536103	23.309710783374832	28.2895996935453
25	27.17870139819958	23.27140394560429	22.29457958245547	27.25531507374066
26	27.523462938134458	24.17161463321203	22.945795824554683	25.35912660409883
27	26.25933729170657	22.926642405669412	24.57383642980272	26.240183872821298
28	26.201877035050757	22.926642405669412	21.815744110323692	29.05573644895614
29	26.64240566941199	23.501244972227543	22.984102662325228	26.87224669603524
30	26.47002489944455	22.773415054587243	24.133307795441485	26.62325225052672
31	27.38938900593756	24.037540701015132	22.90748898678414	25.665581306263167
32	25.89542233288642	22.026431718061673	23.558705228883355	28.51944072016855
33	25.837962076230607	21.60505650258571	26.31679754836238	26.240183872821298
34	26.412564642788738	21.71997701589734	24.99521164527868	26.87224669603524
35	25.56981421183681	24.286535146523654	25.512353955181	24.631296686458533
36	26.987167209346868	22.677647960160886	24.57383642980272	25.76134840068952
37	25.665581306263167	22.69680137904616	25.97203600842751	25.665581306263167
38	24.841984294196514	22.12219881248803	24.76537061865543	28.270446274660028
39	24.343995403179466	20.781459490519058	27.714997126987168	27.159547979314308
40	23.558705228883355	22.562727446849262	28.71097490902126	25.16759241524612
41	23.520398391112813	20.360084275043093	29.20896380003831	26.910553533805786
42	21.73913043478261	20.321777437272555	29.726106109940627	28.212986018004216
43	21.145374449339208	22.409500095767093	28.730128327906527	27.714997126987168
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	2.0
10	2.0
11	2.0
12	2.0
13	3.0
14	4.0
15	12.0
16	20.0
17	19.0
18	18.0
19	18.0
20	17.5
21	17.0
22	13.5
23	10.0
24	11.5
25	13.0
26	13.0
27	13.0
28	13.0
29	20.5
30	28.0
31	30.5
32	33.0
33	33.0
34	49.5
35	66.0
36	82.0
37	98.0
38	102.5
39	107.0
40	107.0
41	137.0
42	167.0
43	196.5
44	226.0
45	315.5
46	405.0
47	405.0
48	453.0
49	501.0
50	577.5
51	654.0
52	628.5
53	603.0
54	603.0
55	545.5
56	488.0
57	451.0
58	414.0
59	405.5
60	397.0
61	397.0
62	360.5
63	324.0
64	261.0
65	198.0
66	156.5
67	115.0
68	115.0
69	111.5
70	108.0
71	92.0
72	76.0
73	61.5
74	47.0
75	47.0
76	39.5
77	32.0
78	23.5
79	15.0
80	10.5
81	6.0
82	6.0
83	5.5
84	5.0
85	4.0
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5221.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.67726489178318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.4725175299706	77.45642597203602
2	5.0441076679484285	8.542424822830876
3	1.5154942320741913	3.8498371959394753
4	0.7690567744854105	2.6048649683968588
5	0.47500565482922413	2.011108982953457
6	0.06785795068988917	0.3447615399348784
7	0.24881248586292692	1.4748132541658685
8	0.13571590137977835	0.9193641064930089
9	0.06785795068988917	0.5171423099023176
>10	0.2035738520696675	2.279256847347251
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	18	0.3447615399348784	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	17	0.32560812104960735	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	14	0.2681478643937943	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	14	0.2681478643937943	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	12	0.22984102662325223	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.22984102662325223	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.22984102662325223	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	10	0.1915341888527102	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	10	0.1915341888527102	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	9	0.1723807699674392	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	9	0.1723807699674392	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	9	0.1723807699674392	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	8	0.15322735108216817	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	8	0.15322735108216817	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	8	0.15322735108216817	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	8	0.15322735108216817	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	8	0.15322735108216817	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	8	0.15322735108216817	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	7	0.13407393219689714	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	7	0.13407393219689714	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	7	0.13407393219689714	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	7	0.13407393219689714	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	7	0.13407393219689714	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	7	0.13407393219689714	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	7	0.13407393219689714	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	7	0.13407393219689714	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.13407393219689714	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	7	0.13407393219689714	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	7	0.13407393219689714	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	6	0.11492051331162612	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	6	0.11492051331162612	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	6	0.11492051331162612	RNA PCR Primer, Index 46 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.01915341888527102	0.0	0.0	0.0	0.0
21	0.01915341888527102	0.0	0.0	0.0	0.0
22	0.01915341888527102	0.0	0.0	0.0	0.0
23	0.01915341888527102	0.0	0.0	0.0	0.0
24	0.01915341888527102	0.0	0.0	0.0	0.0
25	0.01915341888527102	0.0	0.0	0.0	0.0
26	0.01915341888527102	0.0	0.0	0.0	0.0
27	0.01915341888527102	0.0	0.0	0.0	0.0
28	0.01915341888527102	0.0	0.0	0.0957670944263551	0.0
29	0.01915341888527102	0.0	0.0	0.32560812104960735	0.0
30	0.01915341888527102	0.0	0.0	0.5746025665581306	0.0
31	0.01915341888527102	0.0	0.0	1.1492051331162612	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGTCT	50	0.0061575975	18.5	37
>>END_MODULE