FastQCFastQC Report
Fri 10 Feb 2017
ERR1631801.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631801.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646591
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13070.202137054181082No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12660.19579610603921183No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12060.1865166697340359No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11490.17770120524411878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11340.17538134616782478No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10720.165792595319143No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10560.1633180789710961No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10370.1603795908077904No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10120.15651315901396712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.15063618268735568No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9700.15001755360034397No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9480.14661509362177946No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8470.13099470917473333No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8350.12913882191369813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8340.1289841646419452No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7780.12032335742378103No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6650.10284708571569971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.029.7566151
TTAGCTA1002.0008883E-1124.0500014
CGAACTA652.6798316E-622.7692324
GCTCGTT502.7003302E-422.214
GTAGTTA855.1719326E-821.76470620
CTACCGT806.950431E-720.81258
GATACAT450.003824024520.5555575
CTAGCAT909.462201E-820.55555735
GTATTAG1900.020.4473691
TATTAGC1001.2871169E-820.352
TAGTTCG759.255495E-619.73333412
ACTACTA851.2435848E-619.58823627
GTGTATA851.2435848E-619.5882369
TTAACGG851.2435848E-619.58823635
CCGTTTA851.2435848E-619.58823627
GTATCAA14400.019.2708342
ATTAGCT1501.2732926E-1118.53
GCTTCTA500.007031633518.524
CTTATAC6900.018.537
GGACGTA609.230859E-418.527
Produced by FastQC (version 0.11.5)