##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631801.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646591
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.622742970440356	34.0	31.0	34.0	31.0	34.0
2	32.785396023142916	34.0	31.0	34.0	31.0	34.0
3	32.87683558849412	34.0	31.0	34.0	31.0	34.0
4	36.30269521227484	37.0	37.0	37.0	35.0	37.0
5	36.21957311499851	37.0	35.0	37.0	35.0	37.0
6	36.270702809040024	37.0	37.0	37.0	35.0	37.0
7	36.255298944773436	37.0	37.0	37.0	35.0	37.0
8	36.23926098569265	37.0	37.0	37.0	35.0	37.0
9	38.06810023647097	39.0	38.0	39.0	37.0	39.0
10	38.002906010136236	39.0	38.0	39.0	35.0	39.0
11	38.07873911019485	39.0	38.0	39.0	37.0	39.0
12	38.013560349587294	39.0	38.0	39.0	35.0	39.0
13	38.06622579033732	39.0	38.0	39.0	35.0	39.0
14	39.45579663187394	40.0	39.0	41.0	37.0	41.0
15	39.45935529569697	41.0	39.0	41.0	37.0	41.0
16	39.410944476492865	40.0	39.0	41.0	37.0	41.0
17	39.38189674771223	40.0	39.0	41.0	37.0	41.0
18	39.4040483087454	40.0	39.0	41.0	37.0	41.0
19	39.43135305007338	41.0	39.0	41.0	37.0	41.0
20	39.42724535293563	41.0	39.0	41.0	37.0	41.0
21	39.391474672551894	40.0	39.0	41.0	37.0	41.0
22	39.364078683433576	40.0	39.0	41.0	36.0	41.0
23	39.30431602048281	40.0	39.0	41.0	36.0	41.0
24	39.31836044733069	40.0	39.0	41.0	36.0	41.0
25	39.266390964303554	40.0	39.0	41.0	36.0	41.0
26	39.20254534937851	40.0	39.0	41.0	36.0	41.0
27	39.090041463614554	40.0	39.0	41.0	36.0	41.0
28	39.084153661278926	40.0	39.0	41.0	35.0	41.0
29	39.06204849742728	40.0	39.0	41.0	35.0	41.0
30	39.01959662290381	40.0	39.0	41.0	35.0	41.0
31	38.93404176674281	40.0	38.0	41.0	35.0	41.0
32	38.88810701045947	40.0	38.0	41.0	35.0	41.0
33	38.826845718545414	40.0	38.0	41.0	35.0	41.0
34	38.802665054106846	40.0	38.0	41.0	35.0	41.0
35	38.72608495942566	40.0	38.0	41.0	35.0	41.0
36	38.67850619634359	40.0	38.0	41.0	35.0	41.0
37	38.632958083239636	40.0	38.0	41.0	35.0	41.0
38	38.596834784276304	40.0	38.0	41.0	35.0	41.0
39	38.5301867795871	40.0	38.0	41.0	35.0	41.0
40	38.20845635030491	40.0	38.0	41.0	34.0	41.0
41	38.27517549733912	40.0	38.0	41.0	34.0	41.0
42	38.2596633729823	40.0	37.0	41.0	34.0	41.0
43	37.79076881676361	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	4.0
19	15.0
20	19.0
21	45.0
22	92.0
23	175.0
24	300.0
25	525.0
26	862.0
27	1434.0
28	2125.0
29	3015.0
30	4315.0
31	5801.0
32	7974.0
33	10860.0
34	15957.0
35	24007.0
36	37019.0
37	66252.0
38	153151.0
39	312640.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.58951949532239	17.012609207366015	13.05075387687116	27.34711742044043
2	20.289796795810645	19.68787069414823	33.838701744998	26.183630765043127
3	20.74742766292757	20.402851261462036	29.690329744769105	29.159391330841288
4	15.19708749425835	16.40140366939843	34.506666501698916	33.89484233464431
5	16.44687290729379	33.18403751366784	33.97433617232532	16.394753406713054
6	33.0635594989723	34.3187579165191	16.03950565349657	16.57817693101203
7	29.72590091727228	28.35548283226955	20.663294107093975	21.255322143364197
8	26.55991190721801	31.569260939295475	20.600039282947023	21.27078787053949
9	27.915792208675967	13.415435723664574	18.688011432265526	39.980760635393935
10	18.987118595835696	24.05168027392896	30.429900818291628	26.531300311943717
11	37.753231950336456	20.377951440709815	19.693902327746596	22.17491428120713
12	22.41215853607613	24.442035227833358	27.238857330213378	25.90694890587713
13	33.73229754203198	18.464067702767284	21.941536458131957	25.86209829706878
14	23.61276293669414	20.750211493819123	24.072404348343852	31.564621221142886
15	28.772438836915455	24.79016874654921	21.144123564973842	25.293268851561496
16	24.916059765756096	24.766196869427507	23.37752922635793	26.94021413845847
17	25.876790737885308	24.50590868106732	22.912010838381605	26.705289742665766
18	25.638463882114042	22.501550439149323	24.506991281969594	27.352994396767045
19	27.218597227613746	23.10007408083317	24.460284785900203	25.22104390565288
20	28.636959066859884	21.798478481760494	24.0485871284939	25.51597532288572
21	26.41716324539005	23.297262102318157	23.16611273587167	27.119461916420118
22	27.22694872028841	23.70895975972446	22.673993297153842	26.39009822283329
23	26.779061261291915	23.13440799516232	23.835624065290116	26.250906678255653
24	26.904642965955293	22.814422099905503	23.800516864602198	26.480418069537002
25	26.929852101251022	22.85849942235509	24.166281312297883	26.045367164096007
26	26.771173740432513	23.55739563340659	23.661016005481052	26.010414620679846
27	26.24827750463585	22.74405304125792	24.839968388053656	26.167701066052572
28	25.57984877611968	23.68096679353718	23.476355223008053	27.262829207335088
29	26.245802988287803	24.122203989848295	23.687462398950803	25.944530622913092
30	26.039954159584656	23.034190083066424	24.72041831698864	26.205437440360292
31	26.84850237630898	23.147399205989565	23.999715430619975	26.004382987081478
32	24.710365594324696	23.17925860397067	24.14230943517618	27.968066366528454
33	24.91884359664765	22.782717359196152	25.82188740641302	26.476551637743178
34	25.491539473948755	22.632081176508798	25.18887519312827	26.687504156414178
35	25.07334621112883	23.985641618890458	25.293732823376757	25.64727934660396
36	25.321107160477023	22.818597846242834	25.710224856207404	26.15007013707274
37	25.77873802759395	22.477887876571124	25.43895600155276	26.304418094282166
38	24.999729349774434	21.917719238282004	26.468818774155533	26.61373263778803
39	24.569936791573035	21.2526929697444	27.27009809910747	26.907272139575095
40	24.292326988776523	21.998604991408786	27.97038622560475	25.73868179420994
41	22.742661125812145	21.48900928098288	28.813732328473485	26.95459726473149
42	21.70800397778503	21.936742082707617	29.320234893464338	27.03501904604302
43	21.092932008023617	22.515469593607087	29.087321042204422	27.304277356164867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	9.5
2	13.0
3	34.0
4	55.0
5	55.0
6	76.5
7	98.0
8	97.5
9	97.0
10	162.0
11	227.0
12	227.0
13	456.5
14	686.0
15	1275.5
16	1865.0
17	1896.5
18	1928.0
19	1928.0
20	2192.5
21	2457.0
22	2184.5
23	1912.0
24	2101.0
25	2290.0
26	2290.0
27	2646.5
28	3003.0
29	4062.5
30	5122.0
31	6246.5
32	7371.0
33	7371.0
34	9115.0
35	10859.0
36	12448.0
37	14037.0
38	17448.5
39	20860.0
40	20860.0
41	24074.5
42	27289.0
43	31115.5
44	34942.0
45	43332.5
46	51723.0
47	51723.0
48	56423.0
49	61123.0
50	67500.0
51	73877.0
52	73953.0
53	74029.0
54	74029.0
55	66089.5
56	58150.0
57	53690.5
58	49231.0
59	45162.5
60	41094.0
61	41094.0
62	37100.0
63	33106.0
64	27339.5
65	21573.0
66	18595.5
67	15618.0
68	15618.0
69	13298.0
70	10978.0
71	9467.5
72	7957.0
73	6424.5
74	4892.0
75	4892.0
76	4066.5
77	3241.0
78	2694.5
79	2148.0
80	1661.5
81	1175.0
82	1175.0
83	968.5
84	762.0
85	581.0
86	400.0
87	311.0
88	222.0
89	222.0
90	178.5
91	135.0
92	84.0
93	33.0
94	19.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	646591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.93734835316809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.18070704180181	42.17455281826982
2	6.518390523871338	6.37985495537363
3	2.155766139198082	3.1649243556570235
4	1.1043511127253198	2.161760604305911
5	0.703632056294473	1.7216943525669306
6	0.4866858990979467	1.4290270429638623
7	0.34942188828702087	1.1969846468526608
8	0.27537314059494705	1.0780825046720688
9	0.22378593478158487	0.9856341222251198
>10	1.6514486066426248	16.374694487444795
>50	0.2093729986979513	7.068295132528274
>100	0.1296269830333284	11.922548981311607
>500	0.008578256230146722	2.74998205357787
>1k	0.0028594187433822406	1.591963942250456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1307	0.202137054181082	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1266	0.19579610603921183	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1206	0.1865166697340359	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1149	0.17770120524411878	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1134	0.17538134616782478	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1072	0.165792595319143	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1056	0.1633180789710961	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1037	0.1603795908077904	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1012	0.15651315901396712	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	974	0.15063618268735568	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	970	0.15001755360034397	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	948	0.14661509362177946	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	847	0.13099470917473333	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	835	0.12913882191369813	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	834	0.1289841646419452	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	778	0.12032335742378103	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	665	0.10284708571569971	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5465727175293192E-4	0.0	0.0	0.0
3	0.0	1.5465727175293192E-4	0.0	0.0	0.0
4	0.0	1.5465727175293192E-4	0.0	0.0	0.0
5	0.0	3.0931454350586384E-4	0.0	0.0	0.0
6	0.0	3.0931454350586384E-4	0.0	0.0	0.0
7	0.0	3.0931454350586384E-4	0.0	0.0	0.0
8	4.6397181525879573E-4	3.0931454350586384E-4	0.0	0.0	0.0
9	4.6397181525879573E-4	3.0931454350586384E-4	0.0	0.0	0.0
10	4.6397181525879573E-4	3.0931454350586384E-4	0.0	0.0	0.0
11	4.6397181525879573E-4	4.6397181525879573E-4	0.0	0.0	0.0
12	4.6397181525879573E-4	4.6397181525879573E-4	0.0	0.0	0.0
13	4.6397181525879573E-4	4.6397181525879573E-4	0.0	0.0	0.0
14	4.6397181525879573E-4	4.6397181525879573E-4	0.0	0.0	0.0
15	6.186290870117277E-4	4.6397181525879573E-4	0.0	0.0	0.0
16	6.186290870117277E-4	4.6397181525879573E-4	0.0	1.5465727175293192E-4	0.0
17	6.186290870117277E-4	4.6397181525879573E-4	0.0	6.186290870117277E-4	0.0
18	6.186290870117277E-4	4.6397181525879573E-4	0.0	0.0010826009022705235	0.0
19	6.186290870117277E-4	4.6397181525879573E-4	0.0	0.0012372581740234553	0.0
20	6.186290870117277E-4	4.6397181525879573E-4	0.0	0.002010544532788115	0.0
21	7.732863587646596E-4	4.6397181525879573E-4	0.0	0.004175746337329162	0.0
22	7.732863587646596E-4	4.6397181525879573E-4	0.0	0.007732863587646596	0.0
23	9.279436305175915E-4	4.6397181525879573E-4	0.0	0.009743408120434711	0.0
24	9.279436305175915E-4	4.6397181525879573E-4	0.0	0.012527239011987485	0.0
25	9.279436305175915E-4	4.6397181525879573E-4	0.0	0.014228469001269737	0.0
26	0.0010826009022705235	4.6397181525879573E-4	0.0	0.01871352988210476	0.0
27	0.0012372581740234553	4.6397181525879573E-4	0.0	0.03510720068791554	0.0
28	0.0012372581740234553	4.6397181525879573E-4	0.0	0.12279787377182794	0.0
29	0.0012372581740234553	4.6397181525879573E-4	0.0	0.2548751838488318	0.0
30	0.0012372581740234553	4.6397181525879573E-4	0.0	0.43860802269131494	0.0
31	0.0012372581740234553	4.6397181525879573E-4	0.0	0.9795991592830707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	945	0.0	29.756615	1
TTAGCTA	100	2.0008883E-11	24.050001	4
CGAACTA	65	2.6798316E-6	22.76923	24
GCTCGTT	50	2.7003302E-4	22.2	14
GTAGTTA	85	5.1719326E-8	21.764706	20
CTACCGT	80	6.950431E-7	20.8125	8
GATACAT	45	0.0038240245	20.555557	5
CTAGCAT	90	9.462201E-8	20.555557	35
GTATTAG	190	0.0	20.447369	1
TATTAGC	100	1.2871169E-8	20.35	2
TAGTTCG	75	9.255495E-6	19.733334	12
ACTACTA	85	1.2435848E-6	19.588236	27
GTGTATA	85	1.2435848E-6	19.588236	9
TTAACGG	85	1.2435848E-6	19.588236	35
CCGTTTA	85	1.2435848E-6	19.588236	27
GTATCAA	1440	0.0	19.270834	2
ATTAGCT	150	1.2732926E-11	18.5	3
GCTTCTA	50	0.0070316335	18.5	24
CTTATAC	690	0.0	18.5	37
GGACGTA	60	9.230859E-4	18.5	27
>>END_MODULE