FastQCFastQC Report
Fri 10 Feb 2017
ERR1631800.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631800.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119147
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6520.5472231780909297No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5670.4758827330944128No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5650.4742041343886124No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5180.43475706480230303No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4840.4062208868036963No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4790.40202439003919527No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4750.3986671926275945No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4460.3743275113934887No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4250.3567022249825845No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3750.3147372573375746No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3720.312219359278874No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3700.3105407605730736No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3570.29962986898537103No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3170.26605789486936304No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2750.2308073220475547No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2750.2308073220475547No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2650.22241432851855272No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2610.2190571311069519No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2550.21402133498955073No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2400.20143184469604775No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2390.20059254534314755No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2350.19723534793154676No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2330.19555674922574637No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2300.19303885116704575No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2300.19303885116704575No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2240.18800305504964454No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2240.18800305504964454No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2230.18716375569674434No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2200.18464585763804375No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2130.17877076216774238No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2120.17793146281484218No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2080.17457426540324136No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2060.17289566669744097No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2050.17205636734454077No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2020.16953846928584018No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1930.16198477510973838No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1930.16198477510973838No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1910.160306176403938No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1890.1586275776981376No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1860.156109679639437No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1860.156109679639437No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1820.15275248222783622No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1810.15191318287493602No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1780.1493952848162354No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1780.1493952848162354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1760.147716686110435No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1750.1468773867575348No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1740.14603808740463461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.14435948869883422No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1690.1418415906401336No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1680.1410022912872334No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1670.1401629919343332No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1640.13764509387563262No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1610.13512719581693203No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1610.13512719581693203No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1610.13512719581693203No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1590.1334485971111316No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1580.1326092977582314No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1570.1317699984053312No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1530.12841280099373042No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1530.12841280099373042No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1520.12757350164083023No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG1510.12673420228793003No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1510.12673420228793003No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1470.12337700487632924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1440.12085910681762864No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC1410.11834120875892805No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1400.11750190940602785No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1380.11582331070022744No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1380.11582331070022744No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1370.11498401134732725No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1370.11498401134732725No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1360.11414471199442705No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1360.11414471199442705No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1360.11414471199442705No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1350.11330541264152685No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1350.11330541264152685No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1330.11162681393572646No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG1330.11162681393572646No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1310.10994821522992607No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT1300.10910891587702586No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1280.10743031717122546No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1280.10743031717122546No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1270.10659101781832526No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC1250.10491241911252487No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1250.10491241911252487No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1240.10407311975962467No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1230.10323382040672446No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1230.10323382040672446No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1220.10239452105382427No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1220.10239452105382427No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1210.10155522170092407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC200.001835320537.037
CTCCACT200.001835320537.02
ATGCCCC250.005476827729.637
ACCCACT250.005476827729.61
GGTATCA2250.029.61
AGTCGGT556.1828905E-726.9090911
TGGGCAG556.1828905E-726.909096
CAGCACT358.8226044E-426.428574
GGTGATT601.3206663E-624.66666815
CAGTCGG601.3206663E-624.66666810
GGGCAGT601.3206663E-624.6666687
GCAGTCG601.3206663E-624.6666689
AAACACG400.001921096123.12515
GGTCTGT400.001921096123.12536
AAGTGGA400.001921096123.12523
AAAGTGG400.001921096123.12522
GAAAGTG400.001921096123.12521
GTGATTC652.6494763E-622.7692316
GTCGGTG652.6494763E-622.7692312
ACACGAA502.6824474E-422.217
Produced by FastQC (version 0.11.5)