FastQCFastQC Report
Fri 10 Feb 2017
ERR1631799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142910
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3220.22531663284584705No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2880.20152543558883212No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2730.1910293191519138No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2470.17283605066125532No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2400.16793786299069344No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2250.1574417465537751No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2090.1462458890210622No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2030.14204744244629486No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2000.1399482191589112No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2000.1399482191589112No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1970.13784899587152755No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1960.13714925477573298No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1890.13225106710517107No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1880.13155132600937652No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1760.12315443285984187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA200.001836547937.02
GGACCGT250.005480450629.66
TGGGACA250.005480450629.64
GGTATCA1750.029.5999981
AATGAGC855.101174E-821.76470634
CAATGAG855.101174E-821.76470633
TCTTATA1500.020.96666737
TACGGCT450.003809681220.55555530
TGCGGGT909.3334165E-820.55555521
TCAATGA909.3334165E-820.55555532
ATGAGCA909.3334165E-820.55555535
ATCGGAA450.003809681220.55555513
AATCGGA450.003809681220.55555512
TAATACG1001.2661985E-820.3527
AATACGG1001.2661985E-820.3528
GTATCAA2600.019.9230772
CGGGTAA951.6506237E-719.47368423
CTGCGGG951.6506237E-719.47368420
TGAGCAA951.6506237E-719.47368436
TTCTGCG951.6506237E-719.47368418
Produced by FastQC (version 0.11.5)