FastQCFastQC Report
Fri 10 Feb 2017
ERR1631798.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631798.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172203
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43750.3732288690610756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41450.3536076942304362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34690.29593850211951345No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19700.16805962789721576No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18700.15952868231867687No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17470.1490356192570741No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16390.1398221980322521No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16010.13658043871240733No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15890.13555672524298265No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15410.131461871365284No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13460.11482652748713319No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13180.11243786272514232No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13140.11209662490200077No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12660.1080017710243021No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11880.10134763347304178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTA303.5995216E-430.83333420
GGTATCA18350.028.430521
TACGTTA451.3232342E-424.66666619
ATACGGC1005.347829E-1022.19999929
TAGCTAG502.7021472E-422.1999995
AACGCTA450.003825852920.55555533
CTATGCA555.1431765E-420.1818189
CTTATAC11300.019.80973637
TTACGAG801.6167149E-518.56
TATACAC3700.018.537
GTTATAC500.00703496518.4999983
GTATCAA28300.018.3692572
ATTAGAG1950.018.0256423
TATTAGA1750.017.971432
TGCGGGT6300.017.61904721
TAGCGAA852.7231903E-517.41176410
GACCGTT1301.393164E-817.0769237
ATACACA2200.016.81818237
TTCTGCG6650.016.69172918
TACGGCT1452.9795046E-916.58620630
Produced by FastQC (version 0.11.5)