##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631798.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1172203
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893315406973024	31.0	31.0	34.0	30.0	34.0
2	32.0668066879201	33.0	31.0	34.0	30.0	34.0
3	32.14549015827463	34.0	31.0	34.0	30.0	34.0
4	35.83892209796426	37.0	35.0	37.0	35.0	37.0
5	35.53022983220483	37.0	35.0	37.0	33.0	37.0
6	35.55246488876074	37.0	35.0	37.0	33.0	37.0
7	35.55153330950356	37.0	35.0	37.0	33.0	37.0
8	35.51538086833083	37.0	35.0	37.0	33.0	37.0
9	37.24479548337617	39.0	37.0	39.0	34.0	39.0
10	37.07786876505179	39.0	37.0	39.0	33.0	39.0
11	37.17288302452732	39.0	37.0	39.0	34.0	39.0
12	37.09695846197288	39.0	37.0	39.0	33.0	39.0
13	37.18640542636386	39.0	37.0	39.0	34.0	39.0
14	38.336917752300586	40.0	38.0	41.0	34.0	41.0
15	38.313026839207886	40.0	38.0	41.0	34.0	41.0
16	38.24710054487149	40.0	38.0	41.0	34.0	41.0
17	38.22371210447337	40.0	38.0	41.0	34.0	41.0
18	38.258480826273264	40.0	38.0	41.0	34.0	41.0
19	38.28385783008574	40.0	38.0	41.0	34.0	41.0
20	38.26291435869043	40.0	38.0	41.0	34.0	41.0
21	38.22116049865083	40.0	38.0	41.0	34.0	41.0
22	38.18198213108139	40.0	38.0	41.0	34.0	41.0
23	38.11692599319401	40.0	38.0	41.0	34.0	41.0
24	38.12798124556924	40.0	38.0	41.0	34.0	41.0
25	38.0532185978026	40.0	37.0	41.0	34.0	41.0
26	37.95375800949153	40.0	37.0	41.0	33.0	41.0
27	37.83337442405454	40.0	37.0	41.0	33.0	41.0
28	37.81262716440753	40.0	37.0	41.0	33.0	41.0
29	37.76570184515822	39.0	37.0	40.0	33.0	41.0
30	37.69597501456659	39.0	37.0	40.0	33.0	41.0
31	37.588046609674265	39.0	37.0	40.0	33.0	41.0
32	37.51817304681868	39.0	37.0	40.0	33.0	41.0
33	37.43186717658972	39.0	36.0	40.0	33.0	41.0
34	37.373999213446815	39.0	36.0	40.0	32.0	41.0
35	37.26993959237436	39.0	36.0	40.0	32.0	41.0
36	37.21462750052679	39.0	36.0	40.0	32.0	41.0
37	37.14718013859374	39.0	36.0	40.0	32.0	41.0
38	37.09725704506813	39.0	36.0	40.0	32.0	41.0
39	37.02177694477834	39.0	36.0	40.0	31.0	41.0
40	36.693392697339966	39.0	35.0	40.0	31.0	41.0
41	36.736379279015665	39.0	35.0	40.0	31.0	41.0
42	36.67659526549582	39.0	35.0	40.0	31.0	41.0
43	36.11790961121922	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	0.0
14	1.0
15	5.0
16	8.0
17	8.0
18	26.0
19	76.0
20	132.0
21	270.0
22	506.0
23	828.0
24	1424.0
25	2343.0
26	3588.0
27	5623.0
28	8186.0
29	11465.0
30	15846.0
31	21718.0
32	28816.0
33	38940.0
34	55516.0
35	82282.0
36	131291.0
37	225452.0
38	343829.0
39	194021.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.3210826111177	18.006010904254637	13.558999593073896	26.113906891553768
2	19.85338716928723	20.8537258478267	34.212248219804934	25.080638763081136
3	20.82838893945844	21.71279206758556	30.29210810755475	27.166710885401248
4	15.441608663345855	17.01531219421892	34.5341207964832	33.008958345952024
5	15.844866460843388	34.00733490700843	34.090255698031825	16.057542934116363
6	32.46417216130653	35.105950078612665	16.03058514608818	16.39929261399263
7	28.92604779206332	29.473393260382373	20.939291231979443	20.661267715574862
8	26.304146977955185	32.56210741654816	20.38290296134714	20.750842644149518
9	27.56740939922522	13.96481667424499	18.94688889211169	39.5208850344181
10	18.7127144359808	25.006590155459424	30.923312770910837	25.35738263764894
11	37.00195273344293	20.921546865176083	20.445264173526258	21.631236227854732
12	22.065034810523436	25.248783700434142	27.70987619038682	24.976305298655607
13	32.99837997343463	19.02477642524375	22.77361515027687	25.20322845104474
14	23.245376440770073	21.11809985130562	24.95002998627371	30.686493721650603
15	27.970496577811183	25.669274008000322	21.950464211403656	24.409765202784843
16	24.193591041824668	25.504285520511377	24.070830734949492	26.23129270271446
17	25.009575986411907	25.285893313700786	23.80244718704866	25.902083512838647
18	24.888521868652443	23.15673991620905	25.610495792964187	26.344242422174318
19	26.584729778033328	23.759621840244392	25.72139808548519	23.934250296237085
20	27.544973012353662	22.474178960470155	25.395686583296577	24.585161443879603
21	25.61714993051545	24.081153179099523	24.425718071016707	25.875978819368317
22	26.067498547606515	24.385878555164933	24.053598224880844	25.49302467234771
23	25.70689547800168	23.90217394086178	25.008040416207773	25.382890164928774
24	26.180362957610587	23.50070764193574	24.912835063551277	25.406094336902395
25	25.864973899572004	23.530565951460623	25.58788878718106	25.016571361786312
26	25.84765608004757	24.151106932843543	24.915735585047983	25.085501402060906
27	25.538921159560246	23.517428295269678	25.714317400655005	25.22933314451507
28	24.74579914912349	24.313962683937852	24.829658344160524	26.110579822778135
29	25.419658540372275	24.795790490213726	24.747931885518124	25.03661908389588
30	25.113226975191154	24.098726926991315	25.702630005212406	25.085416092605122
31	25.847485461136	23.82718692922642	25.29450956873511	25.03081804090247
32	23.934847462427584	23.93041137072674	25.333837227852175	26.800903938993503
33	24.185742571892412	23.60802693731376	26.63224714490579	25.573983345888042
34	24.6044413808871	23.609391888606325	26.01324173372701	25.772924996779565
35	24.43697891918038	24.6610868595286	26.164068851555577	24.737865369735445
36	24.724130547353997	23.744948613849306	26.43270832782376	25.098212510972928
37	25.11774837634778	23.25313960124654	26.058796983116405	25.57031503928927
38	24.30244590740682	23.020927262598715	26.96819578178865	25.708431048205817
39	23.817546960722673	22.301768550327886	27.844494511616162	26.03618997733328
40	23.715602161059135	23.083459093689402	28.11262213114964	25.088316614101824
41	22.4593351151635	22.654352531088897	28.96563137954774	25.92068097419986
42	21.616392382548074	22.898849431369822	29.243398967584966	26.24135921849714
43	21.037653034499996	23.407549716218096	29.053585428462476	26.50121182081943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	38.0
2	38.0
3	104.5
4	171.0
5	171.0
6	231.5
7	292.0
8	352.0
9	412.0
10	614.5
11	817.0
12	817.0
13	1719.0
14	2621.0
15	5299.0
16	7977.0
17	7863.5
18	7750.0
19	7750.0
20	8666.5
21	9583.0
22	8223.0
23	6863.0
24	7151.0
25	7439.0
26	7439.0
27	8243.0
28	9047.0
29	11790.0
30	14533.0
31	16432.5
32	18332.0
33	18332.0
34	21952.5
35	25573.0
36	28239.5
37	30906.0
38	36881.5
39	42857.0
40	42857.0
41	49388.0
42	55919.0
43	60687.5
44	65456.0
45	79529.5
46	93603.0
47	93603.0
48	100385.5
49	107168.0
50	117410.5
51	127653.0
52	127178.0
53	126703.0
54	126703.0
55	112255.5
56	97808.0
57	89654.0
58	81500.0
59	73489.0
60	65478.0
61	65478.0
62	59143.0
63	52808.0
64	43815.0
65	34822.0
66	30167.0
67	25512.0
68	25512.0
69	21832.5
70	18153.0
71	15538.0
72	12923.0
73	10556.0
74	8189.0
75	8189.0
76	6657.5
77	5126.0
78	4183.0
79	3240.0
80	2678.0
81	2116.0
82	2116.0
83	1690.0
84	1264.0
85	1016.0
86	768.0
87	605.5
88	443.0
89	443.0
90	335.0
91	227.0
92	142.5
93	58.0
94	36.0
95	14.0
96	14.0
97	8.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1172203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.47883079073773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.65615411332446	38.955417391052364
2	6.9687117200848885	6.338577222943432
3	2.277977342679331	3.107992383385431
4	1.1058931340722449	2.0117890686844104
5	0.7299209669915606	1.6597976074210423
6	0.4673131107067685	1.2751712332875846
7	0.3452990170615305	1.0992656898404582
8	0.2648779741042231	0.9637072451583499
9	0.20066285804758097	0.8213320950418583
>10	1.5543194682859391	14.434780903365496
>50	0.23927570116815455	7.6128143751977335
>100	0.1746762939142296	15.120277305572793
>500	0.011141514860597059	3.5301745178400292
>1k	0.0037767846985074776	3.068902961208991
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4375	0.3732288690610756	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4145	0.3536076942304362	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3469	0.29593850211951345	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1970	0.16805962789721576	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1870	0.15952868231867687	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1747	0.1490356192570741	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1639	0.1398221980322521	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1601	0.13658043871240733	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1589	0.13555672524298265	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1541	0.131461871365284	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1346	0.11482652748713319	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1318	0.11243786272514232	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1314	0.11209662490200077	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1266	0.1080017710243021	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1188	0.10134763347304178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	8.530945578538871E-5	0.0	0.0	0.0	0.0
9	8.530945578538871E-5	0.0	0.0	0.0	0.0
10	8.530945578538871E-5	0.0	0.0	0.0	0.0
11	8.530945578538871E-5	0.0	0.0	0.0	0.0
12	8.530945578538871E-5	0.0	0.0	1.7061891157077742E-4	0.0
13	8.530945578538871E-5	0.0	0.0	2.5592836735616615E-4	0.0
14	8.530945578538871E-5	0.0	0.0	2.5592836735616615E-4	0.0
15	1.7061891157077742E-4	0.0	0.0	2.5592836735616615E-4	0.0
16	1.7061891157077742E-4	0.0	0.0	8.530945578538871E-4	0.0
17	1.7061891157077742E-4	0.0	0.0	0.0012796418367808306	0.0
18	1.7061891157077742E-4	0.0	0.0	0.0013649512925662194	0.0
19	1.7061891157077742E-4	0.0	0.0	0.0017061891157077742	0.0
20	1.7061891157077742E-4	0.0	0.0	0.002900521496703216	0.0
21	1.7061891157077742E-4	0.0	0.0	0.004436091700840213	0.0
22	1.7061891157077742E-4	0.0	0.0	0.006398209183904153	0.0
23	1.7061891157077742E-4	0.0	0.0	0.008275017211182704	0.0
24	1.7061891157077742E-4	0.0	0.0	0.010578372517388199	0.0
25	1.7061891157077742E-4	0.0	0.0	0.01117553870788592	0.0
26	1.7061891157077742E-4	0.0	0.0	0.01322296564673525	0.0
27	1.7061891157077742E-4	0.0	0.0	0.02968769061331527	0.0
28	1.7061891157077742E-4	0.0	0.0	0.10203010911932489	0.0
29	1.7061891157077742E-4	0.0	0.0	0.22692315238913396	0.0
30	1.7061891157077742E-4	0.0	0.0	0.3771531040272035	0.0
31	1.7061891157077742E-4	0.0	0.0	0.8483172283299053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCTA	30	3.5995216E-4	30.833334	20
GGTATCA	1835	0.0	28.43052	1
TACGTTA	45	1.3232342E-4	24.666666	19
ATACGGC	100	5.347829E-10	22.199999	29
TAGCTAG	50	2.7021472E-4	22.199999	5
AACGCTA	45	0.0038258529	20.555555	33
CTATGCA	55	5.1431765E-4	20.181818	9
CTTATAC	1130	0.0	19.809736	37
TTACGAG	80	1.6167149E-5	18.5	6
TATACAC	370	0.0	18.5	37
GTTATAC	50	0.007034965	18.499998	3
GTATCAA	2830	0.0	18.369257	2
ATTAGAG	195	0.0	18.025642	3
TATTAGA	175	0.0	17.97143	2
TGCGGGT	630	0.0	17.619047	21
TAGCGAA	85	2.7231903E-5	17.411764	10
GACCGTT	130	1.393164E-8	17.076923	7
ATACACA	220	0.0	16.818182	37
TTCTGCG	665	0.0	16.691729	18
TACGGCT	145	2.9795046E-9	16.586206	30
>>END_MODULE