FastQCFastQC Report
Fri 10 Feb 2017
ERR1631797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057661
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.3232604776010461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31000.2930995848386203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26520.250741967416781No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15320.14484792386218268No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14940.14125509024158026No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14240.13463671251941783No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13110.12395275991078426No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12870.1216836018346143No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12710.12017082978383432No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12700.12007628153066058No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11610.10977052193472199No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11240.1062722365672933No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11010.10409762674429708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.028.196141
CTAGATA400.001931085723.1253
TAATACT1450.020.4137944
CGTTAGA759.264622E-619.7333342
CCGCTTA801.6164715E-518.525
GCAGTCG2150.018.0697679
GTATCAA24300.017.8909452
TTAGATA1351.1514203E-917.8148164
TAGATAC1258.574716E-917.7600025
TACCCTC1054.796366E-717.6190475
GTTTAGG953.6058373E-617.5263161
TCTTATA18700.017.31283437
GCGCATA752.0670694E-417.26666623
TACACTT650.001579821717.0769215
GGATTAG1757.2759576E-1216.9142861
AGTCGGT2350.016.53191611
GTACTGG5150.016.5242731
ATCTCGC1352.2184395E-816.44444511
CTTATAC10050.016.38308537
GATTAGA1251.6572267E-716.282
Produced by FastQC (version 0.11.5)