##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631797.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1057661
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65325751824072	34.0	31.0	34.0	31.0	34.0
2	32.814030204384956	34.0	31.0	34.0	31.0	34.0
3	32.89832848143214	34.0	31.0	34.0	31.0	34.0
4	36.32360274227754	37.0	37.0	37.0	35.0	37.0
5	36.24554275897476	37.0	37.0	37.0	35.0	37.0
6	36.295215574744645	37.0	37.0	37.0	35.0	37.0
7	36.28365326886403	37.0	37.0	37.0	35.0	37.0
8	36.2638444643416	37.0	37.0	37.0	35.0	37.0
9	38.095158089406716	39.0	38.0	39.0	37.0	39.0
10	38.03237615833429	39.0	38.0	39.0	35.0	39.0
11	38.100582322691295	39.0	38.0	39.0	37.0	39.0
12	38.0472987091327	39.0	38.0	39.0	35.0	39.0
13	38.094883899472514	39.0	38.0	39.0	37.0	39.0
14	39.49856901218821	41.0	39.0	41.0	37.0	41.0
15	39.49915899328802	41.0	39.0	41.0	37.0	41.0
16	39.45473171460421	41.0	39.0	41.0	37.0	41.0
17	39.43057179947072	41.0	39.0	41.0	37.0	41.0
18	39.44795165936912	41.0	39.0	41.0	37.0	41.0
19	39.47474001594084	41.0	39.0	41.0	37.0	41.0
20	39.46068730907162	41.0	39.0	41.0	37.0	41.0
21	39.43350657724923	41.0	39.0	41.0	37.0	41.0
22	39.41196753969372	41.0	39.0	41.0	37.0	41.0
23	39.346163846449855	40.0	39.0	41.0	36.0	41.0
24	39.358365298521925	41.0	39.0	41.0	36.0	41.0
25	39.304592870494425	41.0	39.0	41.0	36.0	41.0
26	39.24624241604824	40.0	39.0	41.0	36.0	41.0
27	39.14195663828013	40.0	39.0	41.0	36.0	41.0
28	39.12905458365204	40.0	39.0	41.0	35.0	41.0
29	39.10204309320283	40.0	39.0	41.0	35.0	41.0
30	39.04207113621472	40.0	39.0	41.0	35.0	41.0
31	38.94885884985832	40.0	38.0	41.0	35.0	41.0
32	38.88135612450492	40.0	38.0	41.0	35.0	41.0
33	38.81710207713057	40.0	38.0	41.0	35.0	41.0
34	38.77368079186053	40.0	38.0	41.0	35.0	41.0
35	38.684581354517185	40.0	38.0	41.0	35.0	41.0
36	38.62551138786436	40.0	38.0	41.0	35.0	41.0
37	38.5677187681119	40.0	38.0	41.0	35.0	41.0
38	38.5161181134598	40.0	38.0	41.0	35.0	41.0
39	38.443334868166644	40.0	38.0	41.0	35.0	41.0
40	38.114534808412145	40.0	38.0	41.0	34.0	41.0
41	38.17063312346773	40.0	37.0	41.0	34.0	41.0
42	38.13432092135382	40.0	37.0	41.0	34.0	41.0
43	37.66272463483101	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	0.0
17	3.0
18	7.0
19	23.0
20	43.0
21	91.0
22	166.0
23	274.0
24	491.0
25	884.0
26	1385.0
27	2215.0
28	3481.0
29	4998.0
30	7259.0
31	9784.0
32	13235.0
33	17604.0
34	26022.0
35	38530.0
36	60097.0
37	110892.0
38	242812.0
39	517360.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.8633560280657	17.131481637311012	12.99821020156742	27.00695213305587
2	19.692888364041032	20.126108460083145	34.100813020429044	26.08019015544678
3	20.496075774752022	20.898756785019017	29.672645583036534	28.932521857192427
4	15.281266870953925	16.33727631065152	34.13674135663506	34.24471546175948
5	16.323661362194503	33.448808266542876	33.78795285067711	16.43957752058552
6	33.27493402895635	34.31146652850015	15.816315435664169	16.59728400687933
7	29.78761625889581	28.675634253319355	20.681201254466224	20.855548233318615
8	26.728412979206002	32.02557341151844	20.426488260416146	20.819525348859415
9	27.74688676239362	13.789011791112655	18.664297917763818	39.7998035287299
10	18.764046324862125	24.628212631457526	30.82915981585782	25.778581227822528
11	37.00155342779964	20.808368654984914	20.0427169007839	22.147361016431542
12	22.405288651089528	24.67926868817135	27.663022461828508	25.252420198910613
13	33.125642337194996	18.660610535890044	22.658016131822954	25.555730995092002
14	23.735298928484646	20.6564296121347	24.44866549868058	31.15960596070007
15	28.10976295807447	25.15834468700274	21.42397233139919	25.307920023523607
16	24.87867095411479	24.9665062813132	23.588465491305815	26.5663572732662
17	25.435276520548644	24.825251191071622	23.637063293437123	26.102408994942618
18	25.32191316499332	22.955937677573438	25.17460698654862	26.54754217088462
19	26.815586468632198	23.369775381714934	25.22481210898388	24.589826040668985
20	27.768160119357717	22.432045806737698	24.916490255384286	24.8833038185203
21	26.042087209417765	23.512070502741427	23.94623608131528	26.499606206525534
22	26.47114718232023	24.052791962642097	23.461865380306165	26.014195474731505
23	26.1507231523144	23.567475779101244	24.442708958730634	25.839092109853723
24	26.17946582127922	23.266339592742856	24.550777612108227	26.003416973869697
25	26.209343069282127	23.423006048251754	24.840473459832594	25.527177422633528
26	26.025163072099662	24.029911285374048	24.474193527037492	25.470732115488797
27	25.999540495489576	23.226534778156708	24.973881045060754	25.800043681292966
28	25.261875024227994	24.02310381114554	24.239524762660245	26.475496401966225
29	25.67911646548374	24.316865233756374	24.369150417761457	25.63486788299843
30	25.497772915896494	23.777656545906485	25.313025629194986	25.411544909002032
31	26.11838764972898	23.69672324118976	24.473720785771622	25.711168323309643
32	24.154998624322914	23.74220095096633	24.913464711282725	27.189335713428026
33	24.545388361677322	23.348502024750843	26.181829527608564	25.924280085963268
34	25.157777397483695	23.05965711130504	25.62730402274453	26.15526146846674
35	24.874227186215624	24.162467936323644	25.997933175185622	24.965371702275117
36	24.782704477143433	23.377244693715664	26.242718602652452	25.59733222648845
37	25.141136904925116	22.93277335554587	25.936382262369513	25.989707477159506
38	24.398933117511188	22.627855239060533	26.843383655065278	26.129827988363
39	24.183741293287735	21.997880228163844	27.476478758316702	26.34189972023172
40	23.62533931004358	22.8984523396438	27.975220793808226	25.500987556504402
41	22.52924141100031	22.115498255111987	29.026313724340785	26.328946609546914
42	21.858799747745262	22.50579344421322	29.138164307845333	26.49724250019619
43	21.353155689772056	22.928140491140354	28.99482915603393	26.723874663053664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	40.5
2	41.0
3	103.0
4	165.0
5	165.0
6	238.0
7	311.0
8	317.0
9	323.0
10	524.5
11	726.0
12	726.0
13	1466.5
14	2207.0
15	4189.5
16	6172.0
17	6155.0
18	6138.0
19	6138.0
20	6805.0
21	7472.0
22	6601.5
23	5731.0
24	6132.0
25	6533.0
26	6533.0
27	7336.0
28	8139.0
29	10314.0
30	12489.0
31	14986.0
32	17483.0
33	17483.0
34	20271.0
35	23059.0
36	25545.0
37	28031.0
38	32530.5
39	37030.0
40	37030.0
41	41787.5
42	46545.0
43	51191.0
44	55837.0
45	66732.5
46	77628.0
47	77628.0
48	83268.5
49	88909.0
50	98503.0
51	108097.0
52	109102.0
53	110107.0
54	110107.0
55	100636.0
56	91165.0
57	84561.0
58	77957.0
59	70371.5
60	62786.0
61	62786.0
62	57734.5
63	52683.0
64	44682.0
65	36681.0
66	31848.5
67	27016.0
68	27016.0
69	23341.5
70	19667.0
71	17305.5
72	14944.0
73	12365.5
74	9787.0
75	9787.0
76	8030.5
77	6274.0
78	5078.5
79	3883.0
80	3094.5
81	2306.0
82	2306.0
83	1879.0
84	1452.0
85	1197.0
86	942.0
87	735.5
88	529.0
89	529.0
90	404.0
91	279.0
92	179.0
93	79.0
94	46.5
95	14.0
96	14.0
97	8.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1057661.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.534830049676835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.60762649300237	43.767016858297644
2	6.622543649167365	6.693382356144789
3	2.1185717936795343	3.2118499662490287
4	1.078599398818316	2.180273492438688
5	0.6315250818687445	1.5957006342172626
6	0.4470691126291613	1.3555536976304703
7	0.31668972804706735	1.1202703109735848
8	0.24184899019472605	0.9777438093741153
9	0.19029552225845603	0.8654896688891014
>10	1.4186507241992863	14.460414072126646
>50	0.1879616672088619	6.649891176739664
>100	0.12848879528231832	12.400891780899814
>500	0.007502995291725493	2.526265559387426
>1k	0.0026260483521039226	2.1952566166317737
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3419	0.3232604776010461	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3100	0.2930995848386203	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2652	0.250741967416781	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1532	0.14484792386218268	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1494	0.14125509024158026	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1424	0.13463671251941783	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1311	0.12395275991078426	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1287	0.1216836018346143	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1271	0.12017082978383432	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1270	0.12007628153066058	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1161	0.10977052193472199	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1124	0.1062722365672933	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1101	0.10409762674429708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.454825317374849E-5	0.0	0.0	0.0	9.454825317374849E-5
6	9.454825317374849E-5	0.0	0.0	0.0	9.454825317374849E-5
7	1.8909650634749698E-4	0.0	0.0	0.0	9.454825317374849E-5
8	1.8909650634749698E-4	0.0	0.0	0.0	9.454825317374849E-5
9	1.8909650634749698E-4	0.0	0.0	0.0	9.454825317374849E-5
10	1.8909650634749698E-4	0.0	0.0	0.0	9.454825317374849E-5
11	1.8909650634749698E-4	0.0	0.0	0.0	9.454825317374849E-5
12	1.8909650634749698E-4	0.0	0.0	0.0	9.454825317374849E-5
13	1.8909650634749698E-4	0.0	0.0	9.454825317374849E-5	9.454825317374849E-5
14	1.8909650634749698E-4	0.0	0.0	9.454825317374849E-5	9.454825317374849E-5
15	1.8909650634749698E-4	0.0	0.0	4.7274126586874243E-4	9.454825317374849E-5
16	1.8909650634749698E-4	0.0	0.0	9.454825317374849E-4	9.454825317374849E-5
17	1.8909650634749698E-4	0.0	0.0	0.0014182237976062273	9.454825317374849E-5
18	1.8909650634749698E-4	0.0	0.0	0.001985513316648718	9.454825317374849E-5
19	1.8909650634749698E-4	0.0	0.0	0.002363706329343712	9.454825317374849E-5
20	1.8909650634749698E-4	0.0	0.0	0.0032146406079074487	9.454825317374849E-5
21	1.8909650634749698E-4	0.0	0.0	0.005483798684077413	9.454825317374849E-5
22	1.8909650634749698E-4	0.0	0.0	0.008225698026116118	9.454825317374849E-5
23	1.8909650634749698E-4	0.0	0.0	0.009549373570548598	9.454825317374849E-5
24	1.8909650634749698E-4	0.0	0.0	0.012385821165761052	9.454825317374849E-5
25	1.8909650634749698E-4	0.0	0.0	0.013993141469714776	9.454825317374849E-5
26	1.8909650634749698E-4	0.0	0.0	0.016735040811753484	9.454825317374849E-5
27	1.8909650634749698E-4	0.0	0.0	0.03753565650997815	9.454825317374849E-5
28	3.7819301269499396E-4	0.0	0.0	0.12291272912587303	9.454825317374849E-5
29	3.7819301269499396E-4	0.0	0.0	0.2626550473166733	9.454825317374849E-5
30	3.7819301269499396E-4	0.0	0.0	0.44154034232140543	9.454825317374849E-5
31	3.7819301269499396E-4	0.0	0.0	0.9651485683976245	9.454825317374849E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1555	0.0	28.19614	1
CTAGATA	40	0.0019310857	23.125	3
TAATACT	145	0.0	20.413794	4
CGTTAGA	75	9.264622E-6	19.733334	2
CCGCTTA	80	1.6164715E-5	18.5	25
GCAGTCG	215	0.0	18.069767	9
GTATCAA	2430	0.0	17.890945	2
TTAGATA	135	1.1514203E-9	17.814816	4
TAGATAC	125	8.574716E-9	17.760002	5
TACCCTC	105	4.796366E-7	17.619047	5
GTTTAGG	95	3.6058373E-6	17.526316	1
TCTTATA	1870	0.0	17.312834	37
GCGCATA	75	2.0670694E-4	17.266666	23
TACACTT	65	0.0015798217	17.076921	5
GGATTAG	175	7.2759576E-12	16.914286	1
AGTCGGT	235	0.0	16.531916	11
GTACTGG	515	0.0	16.524273	1
ATCTCGC	135	2.2184395E-8	16.444445	11
CTTATAC	1005	0.0	16.383085	37
GATTAGA	125	1.6572267E-7	16.28	2
>>END_MODULE