FastQCFastQC Report
Fri 10 Feb 2017
ERR1631796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738676
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC17780.24070092977164548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.22892310024963583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16180.21904055363921393No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT13860.1876330082471882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12660.17138772614786454No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12650.17125234879703685No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12240.1657018774131013No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11360.15378867054026393No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10420.1410631995624604No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10300.13943867135252805No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10220.13835565254590648No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9860.13348206791610936No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9400.1272547097780353No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9030.12224574779741051No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8950.12116272899078892No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8360.11317546529195478No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8270.11195706913450552No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT7900.10694810715388074No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG7760.10505282424229297No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT7560.10234527722573902No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG7440.10072074901580667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTACG451.0790791E-732.888894
GGTATCA9250.031.41
CGTTGTT358.8663836E-426.428577
TATGCGT400.001930537323.1253
GCGTTGT400.001930537323.1256
CCATATA400.001930537323.1252
GACAGGC5100.021.401967
CTTTTTA705.0978506E-621.1428572
TCTATCG450.003824532520.55555731
GGACAGG6000.020.356
TCTAATA1550.020.2903232
GCGGTAA3350.019.88059623
ATGCTAG1403.6379788E-1219.82142815
ACAGGCT5600.019.8214288
CATCAGA5600.019.82142816
TAATACT1600.019.656254
ACGTATA951.6740887E-719.4736842
AACGACC1153.0449883E-919.30434837
CCAGGAC6400.018.7890623
CTTATAC8700.018.71264537
Produced by FastQC (version 0.11.5)