##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631796.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	738676
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.597454093540335	34.0	31.0	34.0	31.0	34.0
2	32.762891985119325	34.0	31.0	34.0	31.0	34.0
3	32.847907607665604	34.0	31.0	34.0	31.0	34.0
4	36.28257449815616	37.0	37.0	37.0	35.0	37.0
5	36.20308497907066	37.0	35.0	37.0	35.0	37.0
6	36.25085693863074	37.0	37.0	37.0	35.0	37.0
7	36.23679935452079	37.0	37.0	37.0	35.0	37.0
8	36.21579149721935	37.0	37.0	37.0	35.0	37.0
9	38.047568893533835	39.0	38.0	39.0	35.0	39.0
10	37.984483048048126	39.0	38.0	39.0	35.0	39.0
11	38.05994373717299	39.0	38.0	39.0	35.0	39.0
12	37.99700139167917	39.0	38.0	39.0	35.0	39.0
13	38.04597956343512	39.0	38.0	39.0	35.0	39.0
14	39.434316263151906	40.0	39.0	41.0	37.0	41.0
15	39.437654668623324	40.0	39.0	41.0	37.0	41.0
16	39.39369087394203	40.0	39.0	41.0	37.0	41.0
17	39.36086186636631	40.0	39.0	41.0	36.0	41.0
18	39.35848464008578	40.0	39.0	41.0	36.0	41.0
19	39.377515988065134	40.0	39.0	41.0	36.0	41.0
20	39.36331084264278	40.0	39.0	41.0	36.0	41.0
21	39.33482609425513	40.0	39.0	41.0	36.0	41.0
22	39.30904889288402	40.0	39.0	41.0	36.0	41.0
23	39.24862862743611	40.0	39.0	41.0	36.0	41.0
24	39.25534199026366	40.0	39.0	41.0	36.0	41.0
25	39.18973271095852	40.0	39.0	41.0	36.0	41.0
26	39.115208833101384	40.0	39.0	41.0	35.0	41.0
27	39.02922526249668	40.0	39.0	41.0	35.0	41.0
28	39.009512966442664	40.0	39.0	41.0	35.0	41.0
29	38.98250247740552	40.0	39.0	41.0	35.0	41.0
30	38.920473929029775	40.0	38.0	41.0	35.0	41.0
31	38.84729434826636	40.0	38.0	41.0	35.0	41.0
32	38.797233157703786	40.0	38.0	41.0	35.0	41.0
33	38.730408189788214	40.0	38.0	41.0	35.0	41.0
34	38.680652952038514	40.0	38.0	41.0	35.0	41.0
35	38.595449154974574	40.0	38.0	41.0	35.0	41.0
36	38.54881707270847	40.0	38.0	41.0	35.0	41.0
37	38.49937320286567	40.0	38.0	41.0	35.0	41.0
38	38.467990025396794	40.0	38.0	41.0	34.0	41.0
39	38.39121888351591	40.0	38.0	41.0	34.0	41.0
40	38.05660939302211	40.0	37.0	41.0	34.0	41.0
41	38.115338795358184	40.0	37.0	41.0	34.0	41.0
42	38.08891719779714	40.0	37.0	41.0	34.0	41.0
43	37.60599775815107	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	12.0
20	18.0
21	39.0
22	91.0
23	186.0
24	357.0
25	645.0
26	995.0
27	1671.0
28	2567.0
29	3804.0
30	5413.0
31	7237.0
32	9799.0
33	13178.0
34	19490.0
35	30209.0
36	44542.0
37	78499.0
38	171683.0
39	348235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33347773584088	16.23079672278509	12.500473820727898	27.935251720646132
2	20.402179033838923	18.742452712691357	33.86125987577774	26.994108377691976
3	21.335605867795895	19.914955948210043	28.872875252478757	29.876562931515306
4	15.560543458837161	15.500435915069666	33.34547216912422	35.59354845696895
5	17.136200445120732	32.813168425669716	32.8390254996778	17.211605629531757
6	34.83557066968468	32.96722785091163	15.458062804260598	16.739138675143096
7	30.98787560445987	27.553487591312027	20.007012546772877	21.451624257455233
8	27.786607389437318	31.07560012779622	19.75845973065322	21.37933275211324
9	28.63867243554684	13.025331809886879	17.906632948681153	40.42936280588512
10	19.87447812031256	23.747082618089664	29.48897757609561	26.88946168550217
11	38.33277377361658	19.89857528875989	19.165642311378736	22.603008626244794
12	23.972621284568607	23.49974819812746	26.33685133942351	26.190779177880426
13	33.64831130292578	17.740389561864742	21.577525193724988	27.033773941484498
14	23.901142043331582	20.00254509419556	23.882053836864877	32.21425902560798
15	29.256940796776938	23.857550536365064	20.86029598903985	26.02521267781815
16	25.40328912811571	24.01012081074788	22.230450156766974	28.35613990436944
17	26.233016911338662	24.092700994752775	22.633333152830197	27.040948941078362
18	26.219749930957555	21.57156859028857	24.183809951859814	28.024871526894064
19	27.871895120458767	22.229773270012835	23.60967460699955	26.288657002528847
20	28.086468221520665	21.437680390319976	24.028938262512927	26.446913125646425
21	28.100141333954266	22.240738835429877	22.734189279196833	26.924930551419024
22	28.22617764757485	23.16428312277643	21.98243885005063	26.62710037959809
23	27.65705126469521	22.627105794692127	22.70603079022467	27.00981215038799
24	27.892607855135402	22.116868559422535	22.95471898369515	27.03580460174691
25	27.20001732830091	22.039838846801572	23.869734497939557	26.890409326957965
26	27.665173905744872	22.685318055548034	23.524116121276446	26.125391917430647
27	27.620228625270077	22.048638374605375	23.71080148806784	26.620331512056705
28	26.04700843130141	22.54032891281157	23.47754631259172	27.935116343295302
29	27.209899874911326	22.864422290693078	23.539819893972457	26.385857940423136
30	26.347275395437244	21.908658193849536	24.64314530321819	27.10092110749503
31	26.941852720272486	22.44867844630122	23.56554159062972	27.043927242796574
32	25.27143158840953	22.421061466732368	23.785800540426386	28.52170640443171
33	25.7453876936573	22.49768504730085	25.0415608467041	26.715366412337747
34	26.172097103466204	22.48049212374573	24.400549090534955	26.946861682253115
35	26.08924616475965	23.406067071354695	24.845263688003943	25.659423075881715
36	26.286626342266434	22.420113825276573	24.438048616714227	26.855211215742763
37	25.520390536581665	22.32291288738229	24.991877358950337	27.164819217085707
38	25.93220843779952	21.337636528058308	25.61664383302016	27.11351120112201
39	24.519951913964984	21.122115785540615	26.78115980484001	27.57677249565439
40	24.884387742393148	21.535964347020887	27.402947977191623	26.176699933394342
41	23.288424153485426	20.87491674292924	28.33068896241383	27.505970141171503
42	22.089928466607823	21.786953955455438	28.43790782426937	27.685209753667372
43	21.827702538054574	21.296617190757516	28.745485165349898	28.130195105838013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	20.0
2	23.0
3	52.0
4	81.0
5	81.0
6	95.0
7	109.0
8	138.5
9	168.0
10	271.5
11	375.0
12	375.0
13	714.5
14	1054.0
15	1962.5
16	2871.0
17	2773.5
18	2676.0
19	2676.0
20	2974.5
21	3273.0
22	2666.0
23	2059.0
24	2183.5
25	2308.0
26	2308.0
27	2642.0
28	2976.0
29	3685.0
30	4394.0
31	5572.5
32	6751.0
33	6751.0
34	9073.5
35	11396.0
36	13275.0
37	15154.0
38	18518.5
39	21883.0
40	21883.0
41	24942.0
42	28001.0
43	32859.0
44	37717.0
45	45514.0
46	53311.0
47	53311.0
48	57282.0
49	61253.0
50	68479.5
51	75706.0
52	76380.5
53	77055.0
54	77055.0
55	72376.5
56	67698.0
57	62684.0
58	57670.0
59	52683.0
60	47696.0
61	47696.0
62	45792.0
63	43888.0
64	35484.5
65	27081.0
66	24067.5
67	21054.0
68	21054.0
69	18305.5
70	15557.0
71	14440.5
72	13324.0
73	14461.0
74	15598.0
75	15598.0
76	13412.0
77	11226.0
78	7672.0
79	4118.0
80	2757.0
81	1396.0
82	1396.0
83	1128.5
84	861.0
85	673.5
86	486.0
87	372.0
88	258.0
89	258.0
90	190.0
91	122.0
92	75.5
93	29.0
94	16.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	738676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.57906291789221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.50016954225012	38.11517437514419
2	6.880970892880568	6.134944687398155
3	2.24216798811376	2.998612434438213
4	1.1722639705628808	2.090337172004032
5	0.7299862239781051	1.6271050903957252
6	0.5012847726836696	1.340808325274796
7	0.3729126269476396	1.1636866821702703
8	0.295981018365364	1.0555645152169095
9	0.22054366745566878	0.8848467024884059
>10	1.6512319524315933	14.928834033427766
>50	0.2203698817634399	6.969097817833099
>100	0.19714086938883052	17.23621277065203
>500	0.011614500832210085	3.4848212885447123
>1k	0.0033620923461660773	1.9699541050117082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1778	0.24070092977164548	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1691	0.22892310024963583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1618	0.21904055363921393	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	1386	0.1876330082471882	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1266	0.17138772614786454	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1265	0.17125234879703685	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1224	0.1657018774131013	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1136	0.15378867054026393	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1042	0.1410631995624604	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1030	0.13943867135252805	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1022	0.13835565254590648	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	986	0.13348206791610936	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	940	0.1272547097780353	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	903	0.12224574779741051	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	895	0.12116272899078892	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	836	0.11317546529195478	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	827	0.11195706913450552	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	790	0.10694810715388074	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	776	0.10505282424229297	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	756	0.10234527722573902	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	744	0.10072074901580667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	1.3537735082769714E-4
4	0.0	0.0	0.0	0.0	1.3537735082769714E-4
5	1.3537735082769714E-4	0.0	0.0	0.0	1.3537735082769714E-4
6	1.3537735082769714E-4	0.0	0.0	0.0	1.3537735082769714E-4
7	1.3537735082769714E-4	0.0	0.0	0.0	1.3537735082769714E-4
8	4.0613205248309135E-4	0.0	0.0	0.0	1.3537735082769714E-4
9	4.0613205248309135E-4	1.3537735082769714E-4	0.0	0.0	1.3537735082769714E-4
10	4.0613205248309135E-4	1.3537735082769714E-4	0.0	0.0	1.3537735082769714E-4
11	5.415094033107885E-4	1.3537735082769714E-4	0.0	0.0	1.3537735082769714E-4
12	5.415094033107885E-4	1.3537735082769714E-4	0.0	0.0	1.3537735082769714E-4
13	5.415094033107885E-4	1.3537735082769714E-4	0.0	2.7075470165539427E-4	1.3537735082769714E-4
14	5.415094033107885E-4	1.3537735082769714E-4	0.0	5.415094033107885E-4	1.3537735082769714E-4
15	6.768867541384856E-4	1.3537735082769714E-4	0.0	5.415094033107885E-4	1.3537735082769714E-4
16	6.768867541384856E-4	1.3537735082769714E-4	0.0	8.122641049661827E-4	1.3537735082769714E-4
17	6.768867541384856E-4	1.3537735082769714E-4	0.0	0.0014891508591046683	2.7075470165539427E-4
18	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.0017599055607600625	2.7075470165539427E-4
19	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.002166037613243154	2.7075470165539427E-4
20	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.0031136790690370337	2.7075470165539427E-4
21	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.003925943174003217	2.7075470165539427E-4
22	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.005550471383935582	2.7075470165539427E-4
23	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.00703962224304025	2.7075470165539427E-4
24	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.009205659856283405	2.7075470165539427E-4
25	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.01042405601373268	2.7075470165539427E-4
26	9.476414557938799E-4	1.3537735082769714E-4	0.0	0.015839150046840562	2.7075470165539427E-4
27	0.001083018806621577	1.3537735082769714E-4	0.0	0.040342450546653745	2.7075470165539427E-4
28	0.001083018806621577	1.3537735082769714E-4	0.0	0.13551272817852483	2.7075470165539427E-4
29	0.001083018806621577	1.3537735082769714E-4	0.0	0.2790127200558838	2.7075470165539427E-4
30	0.001083018806621577	1.3537735082769714E-4	0.0	0.46827025651300436	2.7075470165539427E-4
31	0.001083018806621577	1.3537735082769714E-4	0.0	0.9921806042161923	2.7075470165539427E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTACG	45	1.0790791E-7	32.88889	4
GGTATCA	925	0.0	31.4	1
CGTTGTT	35	8.8663836E-4	26.42857	7
TATGCGT	40	0.0019305373	23.125	3
GCGTTGT	40	0.0019305373	23.125	6
CCATATA	40	0.0019305373	23.125	2
GACAGGC	510	0.0	21.40196	7
CTTTTTA	70	5.0978506E-6	21.142857	2
TCTATCG	45	0.0038245325	20.555557	31
GGACAGG	600	0.0	20.35	6
TCTAATA	155	0.0	20.290323	2
GCGGTAA	335	0.0	19.880596	23
ATGCTAG	140	3.6379788E-12	19.821428	15
ACAGGCT	560	0.0	19.821428	8
CATCAGA	560	0.0	19.821428	16
TAATACT	160	0.0	19.65625	4
ACGTATA	95	1.6740887E-7	19.473684	2
AACGACC	115	3.0449883E-9	19.304348	37
CCAGGAC	640	0.0	18.789062	3
CTTATAC	870	0.0	18.712645	37
>>END_MODULE