FastQCFastQC Report
Fri 10 Feb 2017
ERR1631795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822449
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20880.25387592422144106No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18520.2251811358515847No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18200.2212903170895703No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17290.21022580123509177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17080.20767245142251983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16270.1978238164311708No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15280.18578659588618868No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14520.17654590132640444No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13960.1697369684928792No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13680.16633250207611658No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13650.16596773781717772No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12840.15611910282582872No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12480.15174193171856248No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11880.14444664653978545No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11870.1443250584534725No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11770.14310917759034297No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA11640.14152853246827463No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11610.1411637682093358No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11500.13982629925989332No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10990.13362530685793284No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10740.13058560470010908No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10290.1251141408160263No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC10020.12183126248557662No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9780.11891314841406579No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9730.11830520798250105No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9400.11429280113417367No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9300.11307692027104416No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9210.11198262749422759No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8880.10797022064590023No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8610.10468734231545056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.031.4033621
TATACTC250.005495444429.65
TTTTACG802.723391E-823.1250024
CTATCGC450.003824896220.55555530
TACATCG450.003824896220.5555555
CTTATAC9700.020.21649437
GCAGTCG2200.020.1818189
CAGTCGG2300.020.10869610
CTATCTA656.899079E-519.92307720
ATTACCT851.244427E-619.58823633
TTGCGCG851.244427E-619.58823618
TATACAC2550.019.58823637
TCGCCAT1153.0468073E-919.30434813
GTATCAA19500.019.1641032
GCGGTAA4950.019.06060623
CGCGGTA5250.018.67619122
ACTATAC609.2337694E-418.53
GTATTGG609.2337694E-418.51
CCGCTTA801.6157588E-518.525
ATTTTAC500.007033220518.58
Produced by FastQC (version 0.11.5)