FastQCFastQC Report
Fri 10 Feb 2017
ERR1631794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369044
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11320.30673849188714625No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9980.2704284583952049No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9350.2533573232460086No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8880.2406217144839098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.24035074408471618No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8860.24007977368552258No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8610.23330551370568278No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8220.2227376681371327No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8140.22056990494358397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8060.21840214175003525No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7820.211898852169389No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7700.20864720737906592No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7570.2051245921895492No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7250.19645353941535426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6340.1717952330887374No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5690.1541821571411539No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5340.14469819316937818No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5220.14144654837905507No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5180.1403626667822807No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5180.1403626667822807No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5130.13900781478631274No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4980.13494325879840885No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4950.1341303476008281No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4950.1341303476008281No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4810.13033676201211777No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4760.12898191001614984No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4660.1262722060242139No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4500.12193667963711645No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4450.12058182764114848No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4410.11949794604437412No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4400.11922697564518052No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4320.11705921245163178No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA4320.11705921245163178No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4250.11516241965727665No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG4200.11380756766130869No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4100.11109786366937276No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3990.10811718927824325No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3870.10486554448792013No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3730.10107195889920986No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3700.10025904770162906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATC200.001840317337.07
GGTATCA6900.031.3695661
GTAGCTA250.005491567329.613
ACCTACA250.005491567329.627
CGGAGTA250.005491567329.625
ACGGTAG250.005491567329.610
TAAAAGG358.857941E-426.428574
ATACGGC358.857941E-426.4285729
ACTGTGC451.320835E-424.6666668
ACTATCC400.001928716923.1258
CAGGACA802.7132046E-823.1254
GTATAAA400.001928716923.1251
TATAAAA400.001928716923.1252
AACTCAG502.6972865E-422.217
TAGAACT450.003820960920.5555554
AATATTA450.003820960920.5555552
CCGGTCT450.003820960920.5555559
CACGTAG450.003820960920.55555526
GCTAAAC450.003820960920.5555553
GTATCAA10600.020.4198112
Produced by FastQC (version 0.11.5)