##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369044
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59020604589155	34.0	31.0	34.0	31.0	34.0
2	32.76191727815653	34.0	31.0	34.0	31.0	34.0
3	32.844574088726546	34.0	31.0	34.0	31.0	34.0
4	36.278928799817905	37.0	37.0	37.0	35.0	37.0
5	36.20097874508189	37.0	35.0	37.0	35.0	37.0
6	36.252934609423264	37.0	37.0	37.0	35.0	37.0
7	36.24858282481222	37.0	37.0	37.0	35.0	37.0
8	36.232790669947214	37.0	37.0	37.0	35.0	37.0
9	38.05866509142541	39.0	38.0	39.0	35.0	39.0
10	37.99997019325609	39.0	38.0	39.0	35.0	39.0
11	38.073731045620576	39.0	38.0	39.0	35.0	39.0
12	37.999458059201615	39.0	38.0	39.0	35.0	39.0
13	38.05451653461376	39.0	38.0	39.0	35.0	39.0
14	39.41190481351817	40.0	39.0	41.0	37.0	41.0
15	39.425334106502206	40.0	39.0	41.0	37.0	41.0
16	39.37215345595647	40.0	39.0	41.0	36.0	41.0
17	39.34780676558893	40.0	39.0	41.0	36.0	41.0
18	39.376876470014416	40.0	39.0	41.0	36.0	41.0
19	39.37975146594986	40.0	39.0	41.0	36.0	41.0
20	39.39248707471196	40.0	39.0	41.0	36.0	41.0
21	39.34826741526756	40.0	39.0	41.0	36.0	41.0
22	39.31922751758598	40.0	39.0	41.0	36.0	41.0
23	39.252790995111695	40.0	39.0	41.0	36.0	41.0
24	39.25555218347947	40.0	39.0	41.0	36.0	41.0
25	39.22101158669427	40.0	39.0	41.0	36.0	41.0
26	39.15687289320515	40.0	39.0	41.0	36.0	41.0
27	39.0371310738015	40.0	39.0	41.0	35.0	41.0
28	39.027533302262064	40.0	39.0	41.0	35.0	41.0
29	39.01567021818536	40.0	39.0	41.0	35.0	41.0
30	38.96045728964568	40.0	38.0	41.0	35.0	41.0
31	38.87310727176163	40.0	38.0	41.0	35.0	41.0
32	38.83149976696546	40.0	38.0	41.0	35.0	41.0
33	38.766832681197904	40.0	38.0	41.0	35.0	41.0
34	38.75009754934371	40.0	38.0	41.0	35.0	41.0
35	38.660216667931195	40.0	38.0	41.0	35.0	41.0
36	38.612935042975906	40.0	38.0	41.0	35.0	41.0
37	38.5682791211888	40.0	38.0	41.0	35.0	41.0
38	38.53652951951529	40.0	38.0	41.0	35.0	41.0
39	38.464047647434995	40.0	38.0	41.0	35.0	41.0
40	38.150933222054825	40.0	37.0	41.0	34.0	41.0
41	38.20482923445443	40.0	37.0	41.0	34.0	41.0
42	38.1864574413891	40.0	37.0	41.0	34.0	41.0
43	37.72438245846024	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.0
20	9.0
21	20.0
22	36.0
23	86.0
24	184.0
25	236.0
26	459.0
27	719.0
28	1132.0
29	1788.0
30	2533.0
31	3482.0
32	4646.0
33	6519.0
34	9673.0
35	15097.0
36	22346.0
37	39494.0
38	86152.0
39	174427.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.162181203325346	16.57309155547848	13.538765025308635	27.725962215887535
2	20.522485123725083	19.51203650513218	33.247255069856166	26.71822330128657
3	21.06903241889856	19.97891850294274	30.04601077378307	28.90603830437563
4	15.17081973965164	16.857610474631752	34.81779950358223	33.15377028213438
5	16.718602659845438	32.439763280259264	33.57729701607396	17.26433704382133
6	31.895112777880147	34.07832128418292	16.778487118067222	17.24807881986972
7	29.38484299975071	28.037578174960164	20.804023368487226	21.773555456801898
8	26.807914503419646	30.411007901496838	21.11265865316873	21.668418941914787
9	28.776785423960288	12.902797498401275	18.44685186590217	39.87356521173627
10	19.848310770531427	23.278254083523915	29.12850500211357	27.744930143831088
11	38.42089290165942	19.881369159233046	18.875256067027237	22.822481872080296
12	22.612208842306067	24.44640747444749	26.790572397871255	26.150811285375188
13	35.207996878421	17.488971504752822	21.399616305914744	25.903415310911438
14	23.457094546991687	21.451371652160717	24.305502866866824	30.786030933980772
15	30.08421760006937	24.21391487193939	20.412742112051678	25.289125415939562
16	24.045371283640975	24.535014794983795	23.330280400169084	28.089333521206143
17	26.293612685750205	24.240740941459553	22.32958671594715	27.136059656843088
18	26.685435882984144	20.789661937329967	23.868698583366754	28.65620359631914
19	27.784762792512545	22.565872904043964	23.922350722407085	25.727013581036406
20	29.643619730980586	20.54687245965251	23.620760668104616	26.188747141262287
21	27.596438365073002	22.229056697846328	22.548801768894766	27.625703168185904
22	28.405555977065067	23.218640595701327	21.69497404103576	26.680829386197853
23	27.927564192887566	21.946976512285797	22.896456791060142	27.229002503766488
24	27.412178493621358	21.74645841688254	23.89173106729821	26.949632022197896
25	27.007619687625322	22.201959657926967	24.304148014870854	26.486272639576853
26	27.436294859149584	23.082071514507753	23.323235169790053	26.158398456552607
27	26.45511104366959	21.992228568951127	25.39426193082668	26.158398456552607
28	25.612122131778325	23.169594953447287	22.954715426886768	28.263567487887624
29	26.644790323105106	23.708555077443343	23.422139365495713	26.22451523395584
30	26.09905593912921	22.03612577362049	24.740139387173347	27.124678900076955
31	27.50214066615363	22.441497490814104	23.599354006568323	26.457007836463948
32	24.78810114783061	22.572105223225414	23.47687538613282	29.162918242811152
33	25.095652550915336	22.164836713237445	25.98009993388322	26.759410801963995
34	25.634883645310584	22.004693207314034	25.003251644790325	27.35717150258506
35	25.307551403084727	23.741342495745766	25.83594368151223	25.115162419657278
36	25.514301817669438	22.57996336480203	25.494520978528307	26.411213839000226
37	25.408081421185553	22.48945925147137	25.655748366048492	26.44671096129459
38	25.210002059375036	21.381732259567965	26.33913571281473	27.069129968242265
39	24.497349909495885	20.053435362720975	27.878247580234333	27.570967147548803
40	24.20334702637084	21.46735890571314	28.642384105960268	25.686909961955756
41	22.316309166386663	20.511104366958953	29.67180065249672	27.500785814157663
42	20.66230584970898	21.3760418811849	30.5058475412146	27.455804727891525
43	19.810645885043517	22.144242962898733	29.915674011770953	28.129437140286797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	10.0
2	9.0
3	28.0
4	47.0
5	47.0
6	53.0
7	59.0
8	65.0
9	71.0
10	122.5
11	174.0
12	174.0
13	352.5
14	531.0
15	1011.0
16	1491.0
17	1408.5
18	1326.0
19	1326.0
20	1474.0
21	1622.0
22	1268.0
23	914.0
24	902.5
25	891.0
26	891.0
27	1044.5
28	1198.0
29	1377.5
30	1557.0
31	1973.0
32	2389.0
33	2389.0
34	3145.5
35	3902.0
36	4407.5
37	4913.0
38	6397.0
39	7881.0
40	7881.0
41	9576.0
42	11271.0
43	14087.0
44	16903.0
45	24634.5
46	32366.0
47	32366.0
48	35831.0
49	39296.0
50	43794.5
51	48293.0
52	47433.0
53	46573.0
54	46573.0
55	40873.0
56	35173.0
57	32478.0
58	29783.0
59	26757.5
60	23732.0
61	23732.0
62	21271.0
63	18810.0
64	14747.0
65	10684.0
66	9223.0
67	7762.0
68	7762.0
69	6596.0
70	5430.0
71	4901.5
72	4373.0
73	4199.5
74	4026.0
75	4026.0
76	3373.5
77	2721.0
78	1928.0
79	1135.0
80	875.5
81	616.0
82	616.0
83	570.0
84	524.0
85	427.5
86	331.0
87	248.0
88	165.0
89	165.0
90	122.5
91	80.0
92	45.0
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	369044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.64494862313098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53115451051728	31.12122328620363
2	8.07991893716389	6.2449610441152315
3	3.115261385041318	3.6116734851763677
4	1.7657102082623746	2.729431211265495
5	1.0988019122911827	2.1231571723745417
6	0.7794319886342829	1.8072665493598004
7	0.5778390562403358	1.563139244859239
8	0.44298772085539717	1.3695390170507769
9	0.37283196062202195	1.2967264766969309
>10	2.718911344666628	21.06827353151786
>50	0.30972998815953273	8.355360564242286
>100	0.1927053093752749	14.411331533910731
>500	0.014014931590929083	3.9913677110380195
>1k	7.007465795464542E-4	0.3065491721890928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1132	0.30673849188714625	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	998	0.2704284583952049	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	935	0.2533573232460086	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	888	0.2406217144839098	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	887	0.24035074408471618	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	886	0.24007977368552258	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	861	0.23330551370568278	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	822	0.2227376681371327	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	814	0.22056990494358397	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	806	0.21840214175003525	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	782	0.211898852169389	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	770	0.20864720737906592	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	757	0.2051245921895492	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	725	0.19645353941535426	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.1717952330887374	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	569	0.1541821571411539	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	534	0.14469819316937818	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	522	0.14144654837905507	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	518	0.1403626667822807	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	518	0.1403626667822807	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	513	0.13900781478631274	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	498	0.13494325879840885	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	495	0.1341303476008281	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	495	0.1341303476008281	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	481	0.13033676201211777	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	476	0.12898191001614984	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	466	0.1262722060242139	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	450	0.12193667963711645	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	445	0.12058182764114848	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	441	0.11949794604437412	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	440	0.11922697564518052	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	432	0.11705921245163178	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	432	0.11705921245163178	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	425	0.11516241965727665	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	420	0.11380756766130869	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	410	0.11109786366937276	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	399	0.10811718927824325	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	387	0.10486554448792013	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	373	0.10107195889920986	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	370	0.10025904770162906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.7097039919359207E-4	0.0	0.0	0.0
5	0.0	2.7097039919359207E-4	0.0	0.0	0.0
6	0.0	2.7097039919359207E-4	0.0	0.0	0.0
7	0.0	2.7097039919359207E-4	0.0	0.0	0.0
8	5.419407983871841E-4	2.7097039919359207E-4	0.0	0.0	0.0
9	5.419407983871841E-4	2.7097039919359207E-4	0.0	0.0	0.0
10	5.419407983871841E-4	2.7097039919359207E-4	0.0	0.0	0.0
11	5.419407983871841E-4	2.7097039919359207E-4	0.0	0.0	0.0
12	5.419407983871841E-4	2.7097039919359207E-4	0.0	2.7097039919359207E-4	0.0
13	5.419407983871841E-4	2.7097039919359207E-4	0.0	2.7097039919359207E-4	0.0
14	5.419407983871841E-4	2.7097039919359207E-4	0.0	5.419407983871841E-4	0.0
15	8.129111975807763E-4	2.7097039919359207E-4	0.0	8.129111975807763E-4	0.0
16	8.129111975807763E-4	2.7097039919359207E-4	0.0	0.0018967927943551446	2.7097039919359207E-4
17	8.129111975807763E-4	2.7097039919359207E-4	0.0	0.0021677631935487366	2.7097039919359207E-4
18	0.0010838815967743683	2.7097039919359207E-4	0.0	0.002438733592742329	2.7097039919359207E-4
19	0.0010838815967743683	2.7097039919359207E-4	0.0	0.002709703991935921	2.7097039919359207E-4
20	0.0010838815967743683	2.7097039919359207E-4	0.0	0.003793585588710289	2.7097039919359207E-4
21	0.0010838815967743683	2.7097039919359207E-4	0.0	0.005961348782259026	2.7097039919359207E-4
22	0.0010838815967743683	2.7097039919359207E-4	0.0	0.010025904770162908	2.7097039919359207E-4
23	0.0010838815967743683	2.7097039919359207E-4	0.0	0.011922697564518052	2.7097039919359207E-4
24	0.0010838815967743683	2.7097039919359207E-4	0.0	0.014903371955647565	2.7097039919359207E-4
25	0.0010838815967743683	2.7097039919359207E-4	0.0	0.016258223951615525	2.7097039919359207E-4
26	0.0010838815967743683	2.7097039919359207E-4	0.0	0.02086472073790659	2.7097039919359207E-4
27	0.0010838815967743683	2.7097039919359207E-4	0.0	0.0514843758467825	2.7097039919359207E-4
28	0.0010838815967743683	2.7097039919359207E-4	0.0	0.18913733863712728	2.7097039919359207E-4
29	0.0010838815967743683	2.7097039919359207E-4	0.0	0.39913939801216114	2.7097039919359207E-4
30	0.0010838815967743683	2.7097039919359207E-4	0.0	0.6730904715968827	2.7097039919359207E-4
31	0.0010838815967743683	2.7097039919359207E-4	0.0	1.4683885932300755	2.7097039919359207E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTATC	20	0.0018403173	37.0	7
GGTATCA	690	0.0	31.369566	1
GTAGCTA	25	0.0054915673	29.6	13
ACCTACA	25	0.0054915673	29.6	27
CGGAGTA	25	0.0054915673	29.6	25
ACGGTAG	25	0.0054915673	29.6	10
TAAAAGG	35	8.857941E-4	26.42857	4
ATACGGC	35	8.857941E-4	26.42857	29
ACTGTGC	45	1.320835E-4	24.666666	8
ACTATCC	40	0.0019287169	23.125	8
CAGGACA	80	2.7132046E-8	23.125	4
GTATAAA	40	0.0019287169	23.125	1
TATAAAA	40	0.0019287169	23.125	2
AACTCAG	50	2.6972865E-4	22.2	17
TAGAACT	45	0.0038209609	20.555555	4
AATATTA	45	0.0038209609	20.555555	2
CCGGTCT	45	0.0038209609	20.555555	9
CACGTAG	45	0.0038209609	20.555555	26
GCTAAAC	45	0.0038209609	20.555555	3
GTATCAA	1060	0.0	20.419811	2
>>END_MODULE