FastQCFastQC Report
Fri 10 Feb 2017
ERR1631793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882073
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.34328224534704044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27210.3084778697454746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22700.25734831470864655No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19550.22163698469401058No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18460.20927973081593018No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16330.18513206956793826No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16240.18411174585323437No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15130.17152775337188644No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15050.17062079895881632No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14210.1610977776215801No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13950.1581501757791022No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13680.15508920463499054No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13550.15361540371375157No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13030.1477202000287958No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12160.13785707078665824No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9750.11053506909292088No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9710.11008159188638583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.027.9636971
GTAGTAT451.3228715E-424.6666661
ATACACA2100.022.9047637
TCTATAC450.003825113620.5555553
GTGTAGA555.1417906E-420.1818181
TCGGGTA1500.019.73333425
CTTATAC12450.018.87148737
TATACAC3850.018.7402637
ATTACCT1103.8486178E-818.533
GTTCCGA1409.458745E-1118.533
CTACCGT1002.8730392E-718.58
TTTTACG701.2187897E-418.54
TCTATCG500.00703361518.531
GTATCAA23200.018.0215512
GGTAAGT1552.0008883E-1117.90322528
GATTAGA1156.399932E-817.695652
CCGCTTA953.604462E-617.52631625
GCGGTAA6050.017.42975223
GTACTAT852.7219527E-517.4117641
TAGCGAA1603.45608E-1117.3437510
Produced by FastQC (version 0.11.5)