##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631793.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882073
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63528302079306	34.0	31.0	34.0	31.0	34.0
2	32.79297178351452	34.0	31.0	34.0	31.0	34.0
3	32.87418728381891	34.0	31.0	34.0	31.0	34.0
4	36.303680080900335	37.0	37.0	37.0	35.0	37.0
5	36.22488501518582	37.0	35.0	37.0	35.0	37.0
6	36.2776629598684	37.0	37.0	37.0	35.0	37.0
7	36.266131034506216	37.0	37.0	37.0	35.0	37.0
8	36.244833477501295	37.0	37.0	37.0	35.0	37.0
9	38.07504821029552	39.0	38.0	39.0	37.0	39.0
10	38.01661767223348	39.0	38.0	39.0	35.0	39.0
11	38.08480930716619	39.0	38.0	39.0	37.0	39.0
12	38.02756121092018	39.0	38.0	39.0	35.0	39.0
13	38.077124002208436	39.0	38.0	39.0	37.0	39.0
14	39.4610434737261	40.0	39.0	41.0	37.0	41.0
15	39.47562389960922	41.0	39.0	41.0	37.0	41.0
16	39.417954069561134	40.0	39.0	41.0	37.0	41.0
17	39.393174941303045	40.0	39.0	41.0	37.0	41.0
18	39.418892767378665	41.0	39.0	41.0	37.0	41.0
19	39.4482758229761	41.0	39.0	41.0	37.0	41.0
20	39.445313483124416	41.0	39.0	41.0	37.0	41.0
21	39.41017240069699	41.0	39.0	41.0	37.0	41.0
22	39.38542048107129	40.0	39.0	41.0	37.0	41.0
23	39.32305602824256	40.0	39.0	41.0	36.0	41.0
24	39.331248093978616	41.0	39.0	41.0	36.0	41.0
25	39.27709271228118	40.0	39.0	41.0	36.0	41.0
26	39.210320461004926	40.0	39.0	41.0	36.0	41.0
27	39.10626784857943	40.0	39.0	41.0	35.0	41.0
28	39.092800709238354	40.0	39.0	41.0	35.0	41.0
29	39.06881176501265	40.0	39.0	41.0	35.0	41.0
30	39.008694291742295	40.0	39.0	41.0	35.0	41.0
31	38.90452717632214	40.0	38.0	41.0	35.0	41.0
32	38.85126401102857	40.0	38.0	41.0	35.0	41.0
33	38.782864910273865	40.0	38.0	41.0	35.0	41.0
34	38.75220531634003	40.0	38.0	41.0	35.0	41.0
35	38.66551634615276	40.0	38.0	41.0	35.0	41.0
36	38.62129438266447	40.0	38.0	41.0	35.0	41.0
37	38.566687791146535	40.0	38.0	41.0	35.0	41.0
38	38.52272544335899	40.0	38.0	41.0	35.0	41.0
39	38.44451196216186	40.0	38.0	41.0	35.0	41.0
40	38.11894707127415	40.0	38.0	41.0	34.0	41.0
41	38.16733422290445	40.0	37.0	41.0	34.0	41.0
42	38.13828107197477	40.0	37.0	41.0	34.0	41.0
43	37.6573106761005	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	1.0
14	1.0
15	0.0
16	6.0
17	4.0
18	6.0
19	21.0
20	47.0
21	79.0
22	145.0
23	277.0
24	422.0
25	756.0
26	1232.0
27	2027.0
28	3097.0
29	4245.0
30	6076.0
31	8290.0
32	11272.0
33	15254.0
34	21698.0
35	32591.0
36	50551.0
37	92917.0
38	201907.0
39	429147.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.301374149305104	16.872979900756512	13.524504207701629	27.301141742236755
2	19.561532889001253	20.08484558534271	34.112369384393354	26.241252141262684
3	20.59466733478975	20.687856900732708	29.997970689500757	28.719505074976787
4	15.13876969366481	16.85960232316373	34.19331506575987	33.8083129174116
5	16.249335372469172	33.24214662505258	33.926783837618885	16.58173416485937
6	32.445273803868844	34.19263484995006	16.45612097864916	16.90597036753194
7	29.44280121940021	28.68209320543765	20.772997246259663	21.10210832890248
8	26.58385417079992	31.710073882773877	20.698286876483014	21.007785069943193
9	28.113546157744313	13.726301564609733	18.615919544074018	39.54423273357194
10	19.13401725254032	24.370091817797395	30.014522607539284	26.481368322123
11	37.24226906389834	20.69908046159445	19.589081629298256	22.469568845208958
12	22.215734978850957	24.789558233842325	27.3305043913599	25.664202395946823
13	33.717617476104586	18.40550612024175	22.32116842937036	25.555707974283308
14	23.64192079340372	21.067870799809086	24.32871202270107	30.961496384086125
15	28.75827737613553	24.93999929711033	21.25288950007539	25.048833826678745
16	24.423375389565262	25.09384143942735	23.734430143536873	26.748353027470518
17	25.64277559793804	24.854858951583374	23.137994247641636	26.364371202836956
18	25.59277973591755	22.60663233088418	24.828443904302706	26.972144028895567
19	27.061479038582974	23.253630935308074	25.1151548681345	24.569735157974453
20	28.52439650686508	21.757269523044013	24.60215877824171	25.116175191849198
21	26.105775825810333	23.38445910939344	23.62026725679167	26.889497808004553
22	26.867844271392507	23.821384397889968	23.174725901370973	26.13604542934655
23	26.46005489341585	23.247622362321486	24.232121377709102	26.06020136655356
24	26.421282592257107	22.93733058374987	24.643198465433134	25.998188358559894
25	26.281838351247572	23.138220986244903	24.872657931939873	25.70728273056765
26	26.263359155081268	23.897001722079693	24.167614245079488	25.672024877759554
27	26.00283649992688	22.813644675667433	25.313777884596856	25.86974093980884
28	25.104498153780924	23.87795567940522	23.983162391321354	27.034383775492504
29	25.882665040195086	24.2838177792541	24.134963886208965	25.698553294341853
30	25.506278958770984	23.304533751741634	25.345521289054307	25.843666000433068
31	26.36199044750264	23.49227331524715	24.3976405580944	25.748095679155806
32	24.244932108793716	23.48830538968997	24.81654012763116	27.450222373885154
33	24.551029223204882	22.879852347821554	26.26358589368454	26.30553253528903
34	25.172633104062815	22.779293777272404	25.671344661949746	26.376728456715032
35	24.593315972714276	24.0746514177398	26.186154660668677	25.14587794887725
36	24.86313491060264	22.955809779916176	26.528529951602646	25.65252535787854
37	25.138055467064518	22.634067701879548	26.12754273172402	26.100334099331914
38	24.445709141987116	21.940927791690708	27.101498402059693	26.51186466426248
39	24.09381082971591	21.166275353627196	28.12374939489135	26.616164421765546
40	23.663120852809236	22.151454584824613	28.55511958760783	25.63030497475832
41	22.006341878733394	21.65909170782917	29.61920385274235	26.715362560695088
42	21.02104927823434	21.960994158079885	29.91441751419667	27.1035390494891
43	20.247984010393697	22.798566558550142	29.710126032652628	27.243323398403536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	23.5
2	23.0
3	73.0
4	123.0
5	123.0
6	160.0
7	197.0
8	231.0
9	265.0
10	458.0
11	651.0
12	651.0
13	1174.0
14	1697.0
15	3569.0
16	5441.0
17	5218.0
18	4995.0
19	4995.0
20	5490.0
21	5985.0
22	4839.0
23	3693.0
24	3735.5
25	3778.0
26	3778.0
27	4099.0
28	4420.0
29	5848.5
30	7277.0
31	8687.0
32	10097.0
33	10097.0
34	12368.0
35	14639.0
36	16332.5
37	18026.0
38	22562.0
39	27098.0
40	27098.0
41	31557.5
42	36017.0
43	40286.5
44	44556.0
45	58392.0
46	72228.0
47	72228.0
48	79134.0
49	86040.0
50	95982.0
51	105924.0
52	105309.5
53	104695.0
54	104695.0
55	91415.5
56	78136.0
57	71478.5
58	64821.0
59	57850.5
60	50880.0
61	50880.0
62	46331.0
63	41782.0
64	34442.0
65	27102.0
66	23438.0
67	19774.0
68	19774.0
69	16891.5
70	14009.0
71	12179.5
72	10350.0
73	8459.5
74	6569.0
75	6569.0
76	5426.0
77	4283.0
78	3547.0
79	2811.0
80	2202.0
81	1593.0
82	1593.0
83	1304.0
84	1015.0
85	792.5
86	570.0
87	433.0
88	296.0
89	296.0
90	223.5
91	151.0
92	92.0
93	33.0
94	20.5
95	8.0
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	882073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.78405365367289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.83716383424205	38.44136150631806
2	6.631520302425072	5.93972722058451
3	2.1683808570872256	2.913266539361745
4	1.1328930284178311	2.029421686741445
5	0.6762236336971525	1.5142017746687462
6	0.495988861254428	1.3327435064425452
7	0.36377153316916894	1.1403814701388837
8	0.2841116723974695	1.0178937904226406
9	0.2302765095570407	0.9281444003264727
>10	1.7295926934455679	15.846352633287776
>50	0.2665803509287343	8.25654249645252
>100	0.16820532976347286	14.18916133292136
>500	0.011468545211090697	3.4658264065375226
>1k	0.0038228484036968995	2.984975235795819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3028	0.34328224534704044	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2721	0.3084778697454746	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2270	0.25734831470864655	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1955	0.22163698469401058	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1846	0.20927973081593018	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1633	0.18513206956793826	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1624	0.18411174585323437	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1513	0.17152775337188644	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1505	0.17062079895881632	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1421	0.1610977776215801	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1395	0.1581501757791022	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1368	0.15508920463499054	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1355	0.15361540371375157	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1303	0.1477202000287958	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1216	0.13785707078665824	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	975	0.11053506909292088	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	971	0.11008159188638583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0	0.0
6	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0	0.0
7	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0	1.1336930163376501E-4
8	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0	1.1336930163376501E-4
9	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0	1.1336930163376501E-4
10	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0	1.1336930163376501E-4
11	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0	1.1336930163376501E-4
12	1.1336930163376501E-4	1.1336930163376501E-4	0.0	1.1336930163376501E-4	1.1336930163376501E-4
13	1.1336930163376501E-4	1.1336930163376501E-4	0.0	1.1336930163376501E-4	1.1336930163376501E-4
14	1.1336930163376501E-4	1.1336930163376501E-4	0.0	2.2673860326753002E-4	1.1336930163376501E-4
15	1.1336930163376501E-4	1.1336930163376501E-4	0.0	5.66846508168825E-4	1.1336930163376501E-4
16	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0010203237147038851	1.1336930163376501E-4
17	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.00249412463594283	1.1336930163376501E-4
18	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0027208632392103603	1.1336930163376501E-4
19	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0034010790490129504	1.1336930163376501E-4
20	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.0045347720653506	1.1336930163376501E-4
21	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.00793585111436355	1.1336930163376501E-4
22	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.01111019156010897	1.1336930163376501E-4
23	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.012583992481347915	1.1336930163376501E-4
24	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.016325179435262162	1.1336930163376501E-4
25	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.01745887245159981	1.1336930163376501E-4
26	1.1336930163376501E-4	1.1336930163376501E-4	0.0	0.021653536612049117	1.1336930163376501E-4
27	2.2673860326753002E-4	1.1336930163376501E-4	0.0	0.04886216900415272	1.1336930163376501E-4
28	2.2673860326753002E-4	1.1336930163376501E-4	0.0	0.1697138445457462	1.1336930163376501E-4
29	2.2673860326753002E-4	1.1336930163376501E-4	0.0	0.3683368610081025	1.1336930163376501E-4
30	2.2673860326753002E-4	1.1336930163376501E-4	0.0	0.5984765433246455	1.1336930163376501E-4
31	3.4010790490129504E-4	1.1336930163376501E-4	0.0	1.300119151136017	1.1336930163376501E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1515	0.0	27.963697	1
GTAGTAT	45	1.3228715E-4	24.666666	1
ATACACA	210	0.0	22.90476	37
TCTATAC	45	0.0038251136	20.555555	3
GTGTAGA	55	5.1417906E-4	20.181818	1
TCGGGTA	150	0.0	19.733334	25
CTTATAC	1245	0.0	18.871487	37
TATACAC	385	0.0	18.74026	37
ATTACCT	110	3.8486178E-8	18.5	33
GTTCCGA	140	9.458745E-11	18.5	33
CTACCGT	100	2.8730392E-7	18.5	8
TTTTACG	70	1.2187897E-4	18.5	4
TCTATCG	50	0.007033615	18.5	31
GTATCAA	2320	0.0	18.021551	2
GGTAAGT	155	2.0008883E-11	17.903225	28
GATTAGA	115	6.399932E-8	17.69565	2
CCGCTTA	95	3.604462E-6	17.526316	25
GCGGTAA	605	0.0	17.429752	23
GTACTAT	85	2.7219527E-5	17.411764	1
TAGCGAA	160	3.45608E-11	17.34375	10
>>END_MODULE