FastQCFastQC Report
Fri 10 Feb 2017
ERR1631792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864492
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22940.26535815253351097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19810.2291519181206998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16590.19190460987493232No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14450.16715018762463968No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13630.1576648482577051No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13570.15697079903573427No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12410.14355251407763173No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12110.1400822679677776No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11990.13869416952383595No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11350.13129097782281385No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11200.1295558547678868No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11000.1272423573613174No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10550.12203698819653623No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9680.11197327447795931No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9580.1108165257746746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC303.598746E-430.8333343
GGTATCA14050.027.38791
TAATACT1355.638867E-1119.1851864
CTTATAC9850.019.1573637
GCGGTAA4800.018.88541823
TAGACAG500.007033505518.55
TCTAATA1205.167749E-918.52
TATACAC2950.018.18644137
CTCTATG5450.017.3119261
TGCACGA752.06652E-417.26666615
CGCGCAA650.001579505517.07692321
TATACTT650.001579505517.0769235
GTATCAA22300.017.0067252
CTAATAC1851.8189894E-1217.03
CGCGGTA5450.016.97247722
CGGTAAT5400.016.78703724
TCTTATA17350.016.52737637
GACCGTT904.443906E-516.4444457
GTACTGG4850.016.4020611
CACGAAT803.3813514E-416.187517
Produced by FastQC (version 0.11.5)