##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631792.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	864492
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.614814249293225	34.0	31.0	34.0	31.0	34.0
2	32.77558728131666	34.0	31.0	34.0	31.0	34.0
3	32.86482003303674	34.0	31.0	34.0	31.0	34.0
4	36.295621012108846	37.0	37.0	37.0	35.0	37.0
5	36.21033161671826	37.0	35.0	37.0	35.0	37.0
6	36.26350041411604	37.0	37.0	37.0	35.0	37.0
7	36.249519949288135	37.0	37.0	37.0	35.0	37.0
8	36.22856081953332	37.0	37.0	37.0	35.0	37.0
9	38.053409401128064	39.0	38.0	39.0	35.0	39.0
10	37.99185880262628	39.0	38.0	39.0	35.0	39.0
11	38.06445750799313	39.0	38.0	39.0	36.0	39.0
12	38.003653012404975	39.0	38.0	39.0	35.0	39.0
13	38.05371015579092	39.0	38.0	39.0	35.0	39.0
14	39.44493297798013	40.0	39.0	41.0	37.0	41.0
15	39.44805966972511	40.0	39.0	41.0	37.0	41.0
16	39.39877292097555	40.0	39.0	41.0	37.0	41.0
17	39.37159858043799	40.0	39.0	41.0	37.0	41.0
18	39.39386946322233	40.0	39.0	41.0	37.0	41.0
19	39.426465484932194	41.0	39.0	41.0	37.0	41.0
20	39.40912466512125	40.0	39.0	41.0	37.0	41.0
21	39.3805471884066	40.0	39.0	41.0	37.0	41.0
22	39.35277133854333	40.0	39.0	41.0	36.0	41.0
23	39.29435552902745	40.0	39.0	41.0	36.0	41.0
24	39.30035211430528	40.0	39.0	41.0	36.0	41.0
25	39.248902245480586	40.0	39.0	41.0	36.0	41.0
26	39.185845560167124	40.0	39.0	41.0	36.0	41.0
27	39.09203786732555	40.0	39.0	41.0	36.0	41.0
28	39.07913549228911	40.0	39.0	41.0	35.0	41.0
29	39.04836250653563	40.0	39.0	41.0	35.0	41.0
30	38.991846078390545	40.0	39.0	41.0	35.0	41.0
31	38.90243287387275	40.0	38.0	41.0	35.0	41.0
32	38.8481836731861	40.0	38.0	41.0	35.0	41.0
33	38.78791359549886	40.0	38.0	41.0	35.0	41.0
34	38.75522387714403	40.0	38.0	41.0	35.0	41.0
35	38.67878245258487	40.0	38.0	41.0	35.0	41.0
36	38.626839808812576	40.0	38.0	41.0	35.0	41.0
37	38.5830048167016	40.0	38.0	41.0	35.0	41.0
38	38.54662275648589	40.0	38.0	41.0	35.0	41.0
39	38.47685577194468	40.0	38.0	41.0	35.0	41.0
40	38.16101247900501	40.0	38.0	41.0	34.0	41.0
41	38.21756939335471	40.0	38.0	41.0	34.0	41.0
42	38.192021441493964	40.0	37.0	41.0	34.0	41.0
43	37.72388755477205	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	4.0
18	9.0
19	13.0
20	39.0
21	86.0
22	133.0
23	280.0
24	452.0
25	768.0
26	1190.0
27	1853.0
28	2941.0
29	4234.0
30	6038.0
31	8154.0
32	10939.0
33	14856.0
34	21572.0
35	31945.0
36	50035.0
37	90771.0
38	202871.0
39	415306.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.33098744696307	17.12173160653887	13.240955381889016	27.30632556460904
2	19.738528523109526	19.942578994368947	34.07480925213883	26.2440832303827
3	20.53390893148809	20.78561744932284	29.801548192464477	28.878925426724596
4	15.238429042721044	16.531211393512027	34.26197119233029	33.96838837143664
5	16.495236508839874	33.19232566640293	33.687876810890096	16.624561013867105
6	33.098629021436864	34.246702109446936	16.03334675161829	16.621322117497904
7	29.841687372468456	28.474641754926594	20.73113458539813	20.952536287206822
8	26.8291667245041	31.790924612373512	20.354844232219616	21.025064430902773
9	28.04456258704534	13.769589539290125	18.67131216945906	39.51453570420548
10	18.90474405778191	24.52492330756097	30.61254470833738	25.957787926319735
11	37.296470065657054	20.660688589368092	19.843908329978763	22.19893301499609
12	22.526177223155333	24.664080176566124	27.306903938960687	25.50283866131786
13	33.4071339006029	18.657084160408658	22.20726160565974	25.72852033332871
14	23.67216816349949	20.725466516752032	24.297506512495197	31.30485880725328
15	28.37631811514739	25.188203014024424	21.247044507063105	25.188434363765076
16	24.808326739865723	25.026605220175547	23.442784895638134	26.722283144320595
17	25.557668549853556	24.74192936429718	23.34503963021057	26.355362455638687
18	25.592949385303736	22.74630650139041	24.78889336165054	26.87185075165531
19	26.983939701003596	23.33023324680853	24.9516479042027	24.734179147985174
20	28.218768941760015	22.20633620669711	24.348519130310056	25.22637572123282
21	26.253915594360617	23.498424508266126	23.68720589664219	26.560454000731067
22	26.792729140350634	23.981482766757818	23.217450248238272	26.00833784465328
23	26.427312224982995	23.31137824294499	24.245452820847387	26.01585671122463
24	26.47300379876274	23.168751127829985	24.336141919184907	26.022103154222364
25	26.390411941348212	23.16782572886736	24.562401965547398	25.87936036423703
26	26.11707222276204	23.851001513027303	24.23400100868487	25.79792525552579
27	25.92412653905415	23.064412394793703	25.10329765920332	25.90816340694882
28	25.338464670581107	23.781249566219238	24.024860843130995	26.855424920068664
29	26.01550968661364	24.232381560500272	24.045219620308806	25.70688913257728
30	25.646969549747133	23.425086640477875	25.101099836667085	25.826843973107906
31	26.296599621511824	23.558575440836933	24.28455092701841	25.860274010632832
32	24.354765573307795	23.512189817835214	24.69693183973941	27.43611276911759
33	24.669632570341886	22.99882474331746	26.030316070015687	26.301226616324964
34	25.16321724203347	22.82033841840063	25.60197202518936	26.414472314376535
35	24.910351975495434	23.940302512920884	25.8215229290728	25.327822582510883
36	25.00381727072084	22.894370335410855	26.169588613891165	25.932223779977143
37	25.19051651143099	22.464869541881242	26.028118247479444	26.31649569920832
38	24.508728825714986	21.961915205693057	26.98336132665195	26.545994641940005
39	24.242792298829833	21.26335466377942	27.830448402067343	26.663404635323403
40	23.759271340856827	22.13542751118576	28.427562082702906	25.677739065254507
41	22.330223992818905	21.451210653192856	29.507618346959834	26.71094700702841
42	21.42865405347881	21.9417877782559	29.698828907612796	26.930729260652498
43	20.81650263970054	22.34017203166715	29.66273834807031	27.180586980561994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	23.0
2	25.0
3	62.5
4	100.0
5	100.0
6	131.0
7	162.0
8	173.0
9	184.0
10	329.5
11	475.0
12	475.0
13	943.5
14	1412.0
15	2784.5
16	4157.0
17	4001.5
18	3846.0
19	3846.0
20	4354.0
21	4862.0
22	4258.5
23	3655.0
24	3911.5
25	4168.0
26	4168.0
27	4781.0
28	5394.0
29	7032.0
30	8670.0
31	10365.0
32	12060.0
33	12060.0
34	14519.5
35	16979.0
36	18970.0
37	20961.0
38	24926.5
39	28892.0
40	28892.0
41	33206.0
42	37520.0
43	41575.5
44	45631.0
45	56333.5
46	67036.0
47	67036.0
48	72883.5
49	78731.0
50	86833.0
51	94935.0
52	94799.5
53	94664.0
54	94664.0
55	85128.0
56	75592.0
57	69880.0
58	64168.0
59	58110.5
60	52053.0
61	52053.0
62	47282.0
63	42511.0
64	35750.0
65	28989.0
66	25211.0
67	21433.0
68	21433.0
69	18411.0
70	15389.0
71	13454.0
72	11519.0
73	9240.5
74	6962.0
75	6962.0
76	5704.5
77	4447.0
78	3631.5
79	2816.0
80	2267.5
81	1719.0
82	1719.0
83	1389.5
84	1060.0
85	869.5
86	679.0
87	522.0
88	365.0
89	365.0
90	274.0
91	183.0
92	120.0
93	57.0
94	33.0
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	864492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.694966683359844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.37358752650862	43.78706141978619
2	6.49799471639355	6.588312513124387
3	2.175344239232669	3.308370111982168
4	1.0900843389087358	2.2104715697212276
5	0.684612972237012	1.7353215909275637
6	0.4669591604466651	1.420348744880002
7	0.33974682967819686	1.2056417947919322
8	0.2833958203787683	1.1493393337844071
9	0.22345992373520562	1.0195464049940142
>10	1.5503443445052096	15.793349491185035
>50	0.18749064270731616	6.665109587404347
>100	0.11758208653950299	10.97104599911483
>500	0.006417735717063594	2.0359744118032075
>1k	0.0029796630114938116	2.1101070265007555
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2294	0.26535815253351097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1981	0.2291519181206998	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1659	0.19190460987493232	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1445	0.16715018762463968	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1363	0.1576648482577051	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1357	0.15697079903573427	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1241	0.14355251407763173	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1211	0.1400822679677776	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1199	0.13869416952383595	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1135	0.13129097782281385	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1120	0.1295558547678868	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1100	0.1272423573613174	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1055	0.12203698819653623	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	968	0.11197327447795931	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	958	0.1108165257746746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.1567487032847036E-4	0.0	0.0	0.0	0.0
7	1.1567487032847036E-4	0.0	0.0	0.0	0.0
8	2.3134974065694072E-4	0.0	0.0	0.0	0.0
9	2.3134974065694072E-4	0.0	0.0	0.0	0.0
10	2.3134974065694072E-4	0.0	0.0	0.0	0.0
11	2.3134974065694072E-4	0.0	0.0	0.0	0.0
12	2.3134974065694072E-4	0.0	0.0	0.0	1.1567487032847036E-4
13	2.3134974065694072E-4	0.0	0.0	1.1567487032847036E-4	1.1567487032847036E-4
14	3.470246109854111E-4	0.0	0.0	2.3134974065694072E-4	1.1567487032847036E-4
15	3.470246109854111E-4	0.0	0.0	3.470246109854111E-4	1.1567487032847036E-4
16	3.470246109854111E-4	0.0	0.0	5.783743516423518E-4	1.1567487032847036E-4
17	3.470246109854111E-4	0.0	0.0	9.253989626277629E-4	1.1567487032847036E-4
18	3.470246109854111E-4	0.0	0.0	0.0010410738329562333	1.1567487032847036E-4
19	3.470246109854111E-4	0.0	0.0	0.0011567487032847037	1.1567487032847036E-4
20	4.6269948131388145E-4	0.0	0.0	0.0017351230549270554	1.1567487032847036E-4
21	5.783743516423518E-4	0.0	0.0	0.004511319942810344	1.1567487032847036E-4
22	5.783743516423518E-4	0.0	0.0	0.007634541441679044	1.1567487032847036E-4
23	5.783743516423518E-4	0.0	0.0	0.008906965015292218	1.1567487032847036E-4
24	5.783743516423518E-4	0.0	0.0	0.011336137292190095	1.1567487032847036E-4
25	5.783743516423518E-4	0.0	0.0	0.013302610087774091	1.1567487032847036E-4
26	5.783743516423518E-4	0.0	0.0	0.017813930030584436	1.1567487032847036E-4
27	5.783743516423518E-4	0.0	0.0	0.04522887429843191	1.1567487032847036E-4
28	5.783743516423518E-4	0.0	0.0	0.15662377442474887	1.1567487032847036E-4
29	5.783743516423518E-4	0.0	0.0	0.3204193908098629	1.1567487032847036E-4
30	5.783743516423518E-4	0.0	0.0	0.5407800187855989	1.1567487032847036E-4
31	5.783743516423518E-4	0.0	0.0	1.1753723574075874	1.1567487032847036E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAC	30	3.598746E-4	30.833334	3
GGTATCA	1405	0.0	27.3879	1
TAATACT	135	5.638867E-11	19.185186	4
CTTATAC	985	0.0	19.15736	37
GCGGTAA	480	0.0	18.885418	23
TAGACAG	50	0.0070335055	18.5	5
TCTAATA	120	5.167749E-9	18.5	2
TATACAC	295	0.0	18.186441	37
CTCTATG	545	0.0	17.311926	1
TGCACGA	75	2.06652E-4	17.266666	15
CGCGCAA	65	0.0015795055	17.076923	21
TATACTT	65	0.0015795055	17.076923	5
GTATCAA	2230	0.0	17.006725	2
CTAATAC	185	1.8189894E-12	17.0	3
CGCGGTA	545	0.0	16.972477	22
CGGTAAT	540	0.0	16.787037	24
TCTTATA	1735	0.0	16.527376	37
GACCGTT	90	4.443906E-5	16.444445	7
GTACTGG	485	0.0	16.402061	1
CACGAAT	80	3.3813514E-4	16.1875	17
>>END_MODULE