FastQCFastQC Report
Fri 10 Feb 2017
ERR1631791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706603
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.37008051197065395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23020.3257840682816235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20280.28700698978068306No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18700.26464648465970286No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17850.2526170989933527No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17760.2513433993345627No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16190.22912441639789247No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14930.21129262117483227No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14630.20704695564553222No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14360.20322585666916218No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC14330.20280129011623219No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14150.20025389079865213No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13300.18822450513230202No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12670.1793086075207719No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11540.16331660069374176No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10930.15468374745083163No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9620.13614434130622147No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9440.13359694198864142No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9420.1333138976200214No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8750.12383191127125133No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8510.12043537884781129No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8500.1202938566635013No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8350.11817102389885127No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8290.11732189079299127No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8130.11505753584403124No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8010.11335926963231122No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7990.11307622526369121No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7950.11251013652645121No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7820.11067034813042119No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7680.10868903755008118No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7550.10684924915405115No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7530.10656620478543115No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7440.10529250512664112No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7340.10387728328354112No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7280.10302815017768109No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7180.10161292833458109No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7170.10147140615027109No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7140.10104683959734108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.029.7138461
TACCCTC502.7182796E-729.65
TAACGCC250.005494927529.619
TCTGTAC250.005494927529.63
TATCCTT604.317917E-827.7500025
TTGGAGC556.256014E-726.9090933
CATATAA358.866002E-426.428571
ACCCTCA751.3718818E-824.6666666
GTATTAT551.9013143E-523.5454541
CTATACC802.7221176E-823.1250024
TAGCGAA802.7221176E-823.12500210
TTTTACG652.6804173E-622.769234
TATACAC2850.022.719337
AACGTCA4300.022.37209328
GGTCTAT502.7006742E-422.21
GTAGCGA855.1735697E-821.7647069
TTCTGCG4950.021.3030318
GTAACGT4800.021.19791826
CTAAGTC705.097336E-621.14285734
CCTTTGT705.097336E-621.1428578
Produced by FastQC (version 0.11.5)