FastQCFastQC Report
Fri 10 Feb 2017
ERR1631790.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631790.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390016
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9640.2471693468985888No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9530.2443489497866754No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9530.2443489497866754No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8440.21640137840498852No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8170.20947858549392845No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7960.20409419100754841No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7050.18076181489990154No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7010.17973621595011485No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6710.1720442238267148No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6380.16358303249097472No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6360.1630702330160814No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5820.14922464719396125No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5740.14717344929438791No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4700.12050787659993437No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4460.1143542829012143No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4420.11332868395142763No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4320.11076468657696095No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4230.10845708893994094No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4170.10691869051526091No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4070.10435469314079424No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4050.10384189366590088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.10307269445356089No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3940.10102149655398752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT308.2979E-637.010
TTAGTTT200.001840445237.010
ATCAGTT250.00549194729.67
GTTATGG250.00549194729.611
GGTATCA6150.027.6747991
CTAAGTC358.858849E-426.4285734
ATACGGC659.3585186E-825.61538529
TCTTGGA451.3210232E-424.6666662
TAGTTCC400.001928912123.1250024
TTTATCG502.6976687E-422.230
GACCGTT603.7181664E-521.5833327
CTAAGGA603.7181664E-521.5833323
CGGGACG450.003821344620.5555554
TTAAATC450.003821344620.5555553
GCCTAAG555.134724E-420.1818181
TAGTCAC555.134724E-420.18181825
TAAGGAG656.8871974E-519.9230774
CTGTCCG759.240057E-619.73333211
GGTAATA3900.018.9743625
GTATCAA8950.018.8100572
Produced by FastQC (version 0.11.5)