##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631790.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390016
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60020870938628	34.0	31.0	34.0	31.0	34.0
2	32.75960473416475	34.0	31.0	34.0	31.0	34.0
3	32.84973949786676	34.0	31.0	34.0	31.0	34.0
4	36.28576263537906	37.0	37.0	37.0	35.0	37.0
5	36.20221734492944	37.0	35.0	37.0	35.0	37.0
6	36.2490359369872	37.0	37.0	37.0	35.0	37.0
7	36.24125676895307	37.0	37.0	37.0	35.0	37.0
8	36.22352416311126	37.0	37.0	37.0	35.0	37.0
9	38.05295936576961	39.0	38.0	39.0	35.0	39.0
10	37.978562417952084	39.0	38.0	39.0	35.0	39.0
11	38.065699868723335	39.0	38.0	39.0	35.0	39.0
12	37.997615482441745	39.0	38.0	39.0	35.0	39.0
13	38.05821043239252	39.0	38.0	39.0	35.0	39.0
14	39.43300274860518	40.0	39.0	41.0	37.0	41.0
15	39.4443484369872	40.0	39.0	41.0	37.0	41.0
16	39.3820894527404	40.0	39.0	41.0	36.0	41.0
17	39.3480882835576	40.0	39.0	41.0	36.0	41.0
18	39.37205909501149	40.0	39.0	41.0	36.0	41.0
19	39.39215057843781	40.0	39.0	41.0	37.0	41.0
20	39.38607390466032	40.0	39.0	41.0	37.0	41.0
21	39.350295885297015	40.0	39.0	41.0	36.0	41.0
22	39.32369697653429	40.0	39.0	41.0	36.0	41.0
23	39.25662280521825	40.0	39.0	41.0	36.0	41.0
24	39.27912188217919	40.0	39.0	41.0	36.0	41.0
25	39.22420362241549	40.0	39.0	41.0	36.0	41.0
26	39.15814992205448	40.0	39.0	41.0	36.0	41.0
27	39.03382425336397	40.0	39.0	41.0	35.0	41.0
28	39.03323709796521	40.0	39.0	41.0	35.0	41.0
29	39.01090724483098	40.0	39.0	41.0	35.0	41.0
30	38.98073925172301	40.0	38.0	41.0	35.0	41.0
31	38.88716360354447	40.0	38.0	41.0	35.0	41.0
32	38.83962452822448	40.0	38.0	41.0	35.0	41.0
33	38.778311659008864	40.0	38.0	41.0	35.0	41.0
34	38.75996625779455	40.0	38.0	41.0	35.0	41.0
35	38.68540008615031	40.0	38.0	41.0	35.0	41.0
36	38.63622261650804	40.0	38.0	41.0	35.0	41.0
37	38.59417562356416	40.0	38.0	41.0	35.0	41.0
38	38.57031506399738	40.0	38.0	41.0	35.0	41.0
39	38.495415572694455	40.0	38.0	41.0	34.0	41.0
40	38.18332068427962	40.0	38.0	41.0	34.0	41.0
41	38.26072776501477	40.0	37.0	41.0	34.0	41.0
42	38.25329217262882	40.0	37.0	41.0	34.0	41.0
43	37.780042357236624	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	10.0
20	22.0
21	29.0
22	53.0
23	112.0
24	201.0
25	308.0
26	488.0
27	852.0
28	1245.0
29	1850.0
30	2688.0
31	3589.0
32	4816.0
33	6718.0
34	10032.0
35	14840.0
36	22795.0
37	40849.0
38	94816.0
39	183697.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.633122743682314	16.34599606170003	12.847934443715129	28.17294675090253
2	20.79042911060059	19.4812520511979	32.56456145388908	27.163757384312436
3	21.22220626846078	19.625861503117818	29.388794305874633	29.76313792254677
4	15.517568510009847	16.030367984903183	34.29987487692813	34.15218862815885
5	16.842898752871676	32.70942730554644	33.67528511650804	16.772388825073843
6	33.53657285854939	33.91091647522153	15.86755415162455	16.68495651460453
7	30.128507548408272	27.692710042664913	20.181223334427305	21.997559074499506
8	27.28759845749918	30.722842139809647	20.517363390219888	21.472196012471283
9	28.5793403347555	12.760502133245815	17.70081227436823	40.95934525763046
10	19.165367574663602	23.471088365605514	29.824930259271415	27.538613800459466
11	38.874302592714145	19.522276009189365	18.910506235641613	22.692915162454874
12	22.607021250410238	23.960042664916312	26.865564489661963	26.567371595011487
13	35.15189120446341	17.789013784049885	20.866323432884805	26.192771578601903
14	24.2110580078766	20.25275886117493	23.271352970134558	32.264830160813915
15	29.78313710206761	24.142855677715787	19.987128733180175	26.08687848703643
16	25.45741713160486	24.272337545126355	22.549587709222184	27.7206576140466
17	26.88966606498195	23.881584345257632	21.754492123400066	27.474257466360353
18	26.49634886773876	21.59654988513292	23.49749753856252	28.4096037085658
19	27.957827371184774	22.39010707253036	23.5644178700361	26.087647686248772
20	29.975949704627503	20.67966442402363	23.082129963898918	26.262255907449948
21	27.27606046931408	22.314981949458485	21.992943879225468	28.41601370200197
22	28.319863800459466	22.894445356087957	21.4283516573679	27.357339186084673
23	27.64604529044962	22.254728011158516	22.610867246471937	27.48835945191992
24	27.783731949458485	21.551167131604856	23.216227026583525	27.44887389235313
25	27.74860518542829	22.178064489661963	23.212124630784377	26.861205694125367
26	27.70706842796193	22.800859451919923	22.5293321299639	26.96273999015425
27	27.00068715129636	21.705263373810304	24.295669921234	26.998379553659333
28	26.26533270429931	22.936238513291762	22.17139809648835	28.62703068592058
29	27.05376189694782	23.254686987200525	22.56086929766984	27.130681818181817
30	26.66429069576633	22.260112405644897	23.80030562848704	27.275291270101736
31	27.802705530029538	22.566510091893665	22.97264727600919	26.65813710206761
32	25.529209058089926	22.373184689858878	23.223919018706926	28.873687233344274
33	25.843042336724647	22.003969067935675	24.702319904824417	27.45066869051526
34	26.335842632097144	21.8711540039383	24.127728093206432	27.665275270758123
35	25.761763619954053	23.568776665572695	24.61283639645553	26.056623318017724
36	25.9966257794552	22.435746225795864	25.047433951427635	26.5201940433213
37	26.68967426977355	22.140630127994747	24.523096488349196	26.64659911388251
38	25.687151296357076	21.533731949458485	25.704330078765995	27.074786675418444
39	25.257168936659006	20.63889686576961	26.521219642271088	27.582714555300296
40	25.030255169018705	21.54475713816869	27.158629389563504	26.2663583032491
41	23.21520142763374	20.613256892024943	28.323966196258617	27.847575484082704
42	21.99525147686249	21.03682925828684	29.20034049885133	27.767578765999346
43	21.271947817525437	21.77269650475878	28.990605513619954	27.964750164095832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.5
2	5.0
3	9.5
4	14.0
5	14.0
6	23.5
7	33.0
8	32.0
9	31.0
10	47.5
11	64.0
12	64.0
13	133.0
14	202.0
15	461.5
16	721.0
17	699.5
18	678.0
19	678.0
20	749.0
21	820.0
22	712.5
23	605.0
24	691.5
25	778.0
26	778.0
27	956.5
28	1135.0
29	1517.0
30	1899.0
31	2319.5
32	2740.0
33	2740.0
34	3574.5
35	4409.0
36	4994.5
37	5580.0
38	7247.0
39	8914.0
40	8914.0
41	10640.0
42	12366.0
43	14351.5
44	16337.0
45	22909.5
46	29482.0
47	29482.0
48	33008.0
49	36534.0
50	41629.5
51	46725.0
52	47698.5
53	48672.0
54	48672.0
55	43886.5
56	39101.0
57	36712.0
58	34323.0
59	31531.0
60	28739.0
61	28739.0
62	25925.5
63	23112.0
64	18941.0
65	14770.0
66	12687.0
67	10604.0
68	10604.0
69	8963.5
70	7323.0
71	6252.0
72	5181.0
73	4166.0
74	3151.0
75	3151.0
76	2568.5
77	1986.0
78	1657.0
79	1328.0
80	1042.0
81	756.0
82	756.0
83	601.5
84	447.0
85	334.0
86	221.0
87	175.5
88	130.0
89	130.0
90	99.5
91	69.0
92	46.0
93	23.0
94	13.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	390016.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.300699570541774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.7446990016303	43.6331904381808
2	6.071048884822805	6.10756012067089
3	2.0120322060656743	3.036198825706916
4	1.0723928445784536	2.1576844118695795
5	0.6552877950693914	1.6480717256014101
6	0.4875472284128065	1.471437999770573
7	0.34910711794445914	1.2292232580363351
8	0.29435411119576427	1.1844974171689586
9	0.2213496801427447	1.0020639384806145
>10	1.7695805044160282	17.943147142349275
>50	0.2025870384102282	7.092427750147248
>100	0.1133461657940769	10.957506775052888
>500	0.006667421517298641	2.5369901969644983
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	964	0.2471693468985888	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	953	0.2443489497866754	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	953	0.2443489497866754	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	844	0.21640137840498852	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	817	0.20947858549392845	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	796	0.20409419100754841	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	705	0.18076181489990154	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	701	0.17973621595011485	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	671	0.1720442238267148	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	638	0.16358303249097472	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	636	0.1630702330160814	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	582	0.14922464719396125	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	574	0.14717344929438791	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	470	0.12050787659993437	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	446	0.1143542829012143	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	442	0.11332868395142763	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	432	0.11076468657696095	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	423	0.10845708893994094	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	417	0.10691869051526091	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	407	0.10435469314079424	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	405	0.10384189366590088	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	402	0.10307269445356089	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	394	0.10102149655398752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.5639973744666887E-4	0.0
4	0.0	0.0	0.0	2.5639973744666887E-4	0.0
5	0.0	0.0	0.0	2.5639973744666887E-4	0.0
6	0.0	0.0	0.0	2.5639973744666887E-4	0.0
7	0.0	0.0	0.0	2.5639973744666887E-4	0.0
8	2.5639973744666887E-4	0.0	0.0	2.5639973744666887E-4	0.0
9	2.5639973744666887E-4	0.0	0.0	2.5639973744666887E-4	0.0
10	2.5639973744666887E-4	0.0	0.0	2.5639973744666887E-4	0.0
11	2.5639973744666887E-4	0.0	0.0	2.5639973744666887E-4	0.0
12	2.5639973744666887E-4	0.0	0.0	2.5639973744666887E-4	0.0
13	2.5639973744666887E-4	0.0	0.0	2.5639973744666887E-4	0.0
14	2.5639973744666887E-4	0.0	0.0	2.5639973744666887E-4	0.0
15	2.5639973744666887E-4	0.0	0.0	0.0010255989497866755	0.0
16	2.5639973744666887E-4	0.0	0.0	0.0012819986872333444	0.0
17	2.5639973744666887E-4	0.0	0.0	0.0017947981621266819	0.0
18	2.5639973744666887E-4	0.0	0.0	0.002051197899573351	0.0
19	2.5639973744666887E-4	0.0	0.0	0.0033331965868066953	0.0
20	2.5639973744666887E-4	0.0	0.0	0.004358795536593371	0.0
21	2.5639973744666887E-4	0.0	0.0	0.007435592385953396	0.0
22	2.5639973744666887E-4	0.0	0.0	0.010768788972760093	0.0
23	2.5639973744666887E-4	0.0	0.0	0.013589186084673448	0.0
24	2.5639973744666887E-4	0.0	0.0	0.016665982934033475	0.0
25	2.5639973744666887E-4	0.0	0.0	0.019229980308500164	0.0
26	2.5639973744666887E-4	0.0	0.0	0.023845175582540203	0.0
27	2.5639973744666887E-4	0.0	0.0	0.043331555628487034	0.0
28	2.5639973744666887E-4	0.0	0.0	0.12691787003610108	0.0
29	5.127994748933377E-4	0.0	0.0	0.2669121266819823	0.0
30	5.127994748933377E-4	0.0	0.0	0.44741754184443716	0.0
31	5.127994748933377E-4	0.0	0.0	0.9520122251394815	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	30	8.2979E-6	37.0	10
TTAGTTT	20	0.0018404452	37.0	10
ATCAGTT	25	0.005491947	29.6	7
GTTATGG	25	0.005491947	29.6	11
GGTATCA	615	0.0	27.674799	1
CTAAGTC	35	8.858849E-4	26.42857	34
ATACGGC	65	9.3585186E-8	25.615385	29
TCTTGGA	45	1.3210232E-4	24.666666	2
TAGTTCC	40	0.0019289121	23.125002	4
TTTATCG	50	2.6976687E-4	22.2	30
GACCGTT	60	3.7181664E-5	21.583332	7
CTAAGGA	60	3.7181664E-5	21.583332	3
CGGGACG	45	0.0038213446	20.555555	4
TTAAATC	45	0.0038213446	20.555555	3
GCCTAAG	55	5.134724E-4	20.181818	1
TAGTCAC	55	5.134724E-4	20.181818	25
TAAGGAG	65	6.8871974E-5	19.923077	4
CTGTCCG	75	9.240057E-6	19.733332	11
GGTAATA	390	0.0	18.97436	25
GTATCAA	895	0.0	18.810057	2
>>END_MODULE