FastQCFastQC Report
Fri 10 Feb 2017
ERR1631789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221703
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8850.39918269035601683No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6120.27604497909365233No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5730.2584538774847431No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5540.24988385362399246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.24131382976324184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5200.2345480214521229No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4910.22146745871729295No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4890.22056535094247712No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4830.21785902761802955No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4580.2065826804328313No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4400.19846371045948857No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4400.19846371045948857No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4400.19846371045948857No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4360.19665949490985687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4230.19079579437355382No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4170.18808947104910623No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3990.1799705010757635No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3260.14704356729498472No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3150.14208197453349752No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3140.1416309206460896No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3130.14117986675868166No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3080.138924597321642No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3020.13621827399719444No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3000.13531616622237858No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2960.13351195067274688No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2920.13170773512311518No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT2880.12990351957348345No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2850.12855035791125966No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2750.12403981903718038No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2700.12178454960014072No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT2660.11998033405050902No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2550.11501874128902179No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2480.1118613640771663No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2460.11095925630235046No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2450.11050820241494251No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2440.11005714852753458No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2330.10509555576604737No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT2300.10374239410382358No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2300.10374239410382358No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2250.10148712466678395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTTG200.001838733837.07
GGTATCA3950.031.3797451
CTAAGTC250.00548689729.634
CCATATC250.00548689729.635
GCCTAAC250.00548689729.62
TTTTACG250.00548689729.64
TATACAC753.7126665E-722.237
TTGCACA450.003816220720.5555574
AAGTCTG450.003816220720.55555736
CTTATAC2550.019.58823637
ATCAGTA609.2047954E-418.514
CAGTATG500.007017421518.516
TCAGTAT500.007017421518.515
GTATCAA6900.017.9637682
CATCTAA1800.017.47222331
TCTTATA4550.017.07692337
ATCTAAG1851.8189894E-1217.032
AGCCGCG1352.1911546E-816.44444319
CGCGGTA1352.1911546E-816.44444322
TCTAAGT1708.367351E-1116.3235333
Produced by FastQC (version 0.11.5)