FastQCFastQC Report
Fri 10 Feb 2017
ERR1631788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541511
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.4398802609734613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22700.4191973939587561No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA21510.39722184775563196No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19150.35364009226036036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18210.3362812574444471No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17740.32760184003649045No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17010.3141210427858344No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17010.3141210427858344No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16900.3120896897754616No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16470.30414894618945876No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15780.29140682276075647No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14610.2698006134686092No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14440.2666612497253057No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13750.2539191262966034No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13740.253734457841115No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10060.18577646622136948No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA10010.18485312394392728No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9690.17894373336829725No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9620.17765105417987817No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9350.17266500588169031No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9240.17063365287131746No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9190.16971031059387529No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8690.16047688781945335No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8660.15992288245298802No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8320.15364415496638112No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC8230.15198213886698514No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8200.15142813350051984No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8150.15050479122307764No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7960.1469960905687973No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7830.1445954006474476No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7730.14274871609256323No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG7700.1421947107260979No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7670.14164070535963258No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA7640.14108669999316725No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7560.13960935234925975No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7540.13924001543828288No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7210.1331459564071644No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7140.13185327721874532No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA6900.1274212342870228No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6890.12723656583153437No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6870.1268672289205575No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6720.1240972020882309No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6510.12021916452297368No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6470.11948049070101992No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6450.11911115379004304No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6330.11689513232418178No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6170.11394043703636676No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6130.113201763214413No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT6110.11283242630343612No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5890.10876972028269047No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5810.10729237263878295No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5670.10470701426194481No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT5640.1041530088954795No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5640.1041530088954795No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5640.1041530088954795No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5490.10138298206315292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAC200.001841076937.025
CTTTTTA601.2678356E-930.8333322
CGAACTA250.00549380829.624
CCTAGTT751.4551915E-1129.62
TAGTTCC751.4551915E-1129.64
CTAGTTC751.4551915E-1129.63
GCCTAGT751.4551915E-1129.61
TAGTGAT250.00549380829.69
TTTTACG652.9831426E-928.4615384
GGTATCA14550.027.2096211
TACCTTA754.620233E-1027.13333331
GCTTTTT706.5756467E-926.4285721
GAAATTC1300.025.61538514
TAAGGTA1400.025.1071435
TAGCGAA1350.024.66666610
CTAAGTC601.3352255E-624.66666634
TCGCTAC901.4188117E-1024.66666627
TTTTGCT451.32195E-424.6666662
TCGGGTA1400.023.78571525
ACTGTGC701.9190156E-723.7857158
Produced by FastQC (version 0.11.5)