##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631788.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541511
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61694591614944	34.0	31.0	34.0	31.0	34.0
2	32.767733250109416	34.0	31.0	34.0	31.0	34.0
3	32.841984742692205	34.0	31.0	34.0	31.0	34.0
4	36.272694368166114	37.0	37.0	37.0	35.0	37.0
5	36.20409003695216	37.0	35.0	37.0	35.0	37.0
6	36.26528731641647	37.0	37.0	37.0	35.0	37.0
7	36.261361265052784	37.0	37.0	37.0	35.0	37.0
8	36.24139491164538	37.0	37.0	37.0	35.0	37.0
9	38.07282215873731	39.0	38.0	39.0	37.0	39.0
10	38.011918502117226	39.0	38.0	39.0	35.0	39.0
11	38.08521525878514	39.0	38.0	39.0	37.0	39.0
12	38.01730158759471	39.0	38.0	39.0	35.0	39.0
13	38.07929294141763	39.0	38.0	39.0	37.0	39.0
14	39.42352417587085	40.0	39.0	41.0	37.0	41.0
15	39.44809985392725	40.0	39.0	41.0	37.0	41.0
16	39.374607348696514	40.0	39.0	41.0	36.0	41.0
17	39.36793897076883	40.0	39.0	41.0	37.0	41.0
18	39.41520670863565	40.0	39.0	41.0	37.0	41.0
19	39.42990262432342	40.0	39.0	41.0	37.0	41.0
20	39.44810170061181	40.0	39.0	41.0	37.0	41.0
21	39.40963526133356	40.0	39.0	41.0	37.0	41.0
22	39.37371355337196	40.0	39.0	41.0	36.0	41.0
23	39.31902953033272	40.0	39.0	41.0	36.0	41.0
24	39.31266400867203	40.0	39.0	41.0	36.0	41.0
25	39.26417376562988	40.0	39.0	41.0	36.0	41.0
26	39.184319432107564	40.0	39.0	41.0	36.0	41.0
27	39.06665053895489	40.0	39.0	41.0	35.0	41.0
28	39.06187501269596	40.0	39.0	41.0	35.0	41.0
29	39.02649068993982	40.0	39.0	41.0	35.0	41.0
30	38.961062656160266	40.0	38.0	41.0	35.0	41.0
31	38.840887812066605	40.0	38.0	41.0	35.0	41.0
32	38.779533564415125	40.0	38.0	41.0	35.0	41.0
33	38.69540969620192	40.0	38.0	41.0	35.0	41.0
34	38.670778617608875	40.0	38.0	41.0	35.0	41.0
35	38.5722044427537	40.0	38.0	41.0	35.0	41.0
36	38.50699616443618	40.0	38.0	41.0	35.0	41.0
37	38.43207432535996	40.0	38.0	41.0	35.0	41.0
38	38.39307604092992	40.0	38.0	41.0	35.0	41.0
39	38.280858560583255	40.0	38.0	41.0	34.0	41.0
40	37.94763910613081	40.0	37.0	41.0	34.0	41.0
41	37.98220719431369	40.0	37.0	41.0	34.0	41.0
42	37.92937170251389	40.0	37.0	41.0	34.0	41.0
43	37.439710365994415	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	3.0
18	3.0
19	4.0
20	15.0
21	36.0
22	84.0
23	145.0
24	235.0
25	441.0
26	778.0
27	1174.0
28	1879.0
29	2764.0
30	3846.0
31	5284.0
32	7001.0
33	9602.0
34	13912.0
35	20904.0
36	31886.0
37	60985.0
38	130021.0
39	250508.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.6227722059201	16.30825597263952	13.807106411504105	27.26186540993627
2	20.417498444168263	20.3290422539893	33.01151777156882	26.24194153027362
3	21.496885566498186	20.26625497912323	30.341765910572455	27.895093543806126
4	14.884646849279145	17.635098825323954	35.32079680745174	32.15945751794516
5	16.302346582063894	32.707368825379355	34.05175518133519	16.938529411221566
6	30.029122215430526	34.71545361036064	17.40961864117257	17.845805533036263
7	28.568948737883442	28.360458051636993	20.89578974388332	22.174803466596245
8	26.842483347522027	29.94842210038208	21.333823320301896	21.875271231794
9	28.963215890351258	12.862157924769765	18.65520737344209	39.519418811436886
10	19.969123434242334	23.32750396575508	28.66294498172706	28.040427618275526
11	37.81825299947739	20.56948058303525	18.850771267804348	22.761495149683018
12	21.592728494896686	25.131160770510665	26.980800020682867	26.29531071390978
13	35.67240554670173	18.14441442556107	21.199754021617288	24.983426006119913
14	23.409866096902928	22.142486486885772	24.756099137413646	29.691548278797658
15	30.403629843161077	24.332100363612188	20.815458965745847	24.448810827480884
16	23.188633287227773	25.065049463445803	24.3511212145275	27.395196034798925
17	25.994670468374604	24.57697073558986	22.401022324569585	27.02733647146595
18	25.940377942461	21.030782384845367	24.606702356923496	28.42213731577013
19	27.26537411059055	23.15483895987339	25.134854139620433	24.444932789915626
20	29.934387297764957	20.370961993385176	24.32508296230363	25.369567746546238
21	26.560494615991182	22.613575716836777	23.35852826627714	27.467401400894904
22	27.670352033476696	23.12159863788547	23.033696453072974	26.174352875564853
23	26.966026544243793	22.387356858863438	24.13468978469505	26.51192681219772
24	26.79908626048224	21.761515463213122	25.301794423381978	26.137603852922652
25	26.498446014947064	22.441280047866062	25.138178171819227	25.922095765367647
26	26.680713780514154	23.613740071762162	23.906624242166828	25.79892190555686
27	25.72080714888525	22.045904884665315	26.49272129282692	25.74056667362251
28	24.927286795651426	23.657137158801945	23.33913807845085	28.076437967095774
29	26.322087639955605	24.036815503286174	23.727680508798528	25.91341634795969
30	25.837332944298453	22.19567100206644	25.70621834090166	26.260777712733447
31	27.144046935334647	22.871557549154126	24.382884188871508	25.60151132663972
32	24.595068244227726	22.953181006480015	24.344657818585404	28.107092930706855
33	24.5428070713245	22.30831875991439	26.71081473875877	26.438059430002347
34	25.041781238054263	22.78864141263982	25.166986450875424	27.0025908984305
35	24.270790436390026	24.96809852431437	26.02255540515336	24.73855563414224
36	25.143164220117413	23.380873149391242	26.097900134992642	25.378062495498703
37	26.035666865493035	23.445876445723172	25.33521941382539	25.183237274958405
38	25.199303430585896	22.486339151005243	25.864294538799765	26.450062879609092
39	24.93430419695999	21.03743044924295	27.27146816962167	26.75679718417539
40	24.668935626423103	22.673777633326008	27.299907111766892	25.357379628484
41	22.364088633471894	21.864745129831157	28.59406364782987	27.177102588867076
42	20.7715078733396	22.093180009270355	29.52128396283732	27.614028154552724
43	20.16819602925887	23.57828372830838	28.886947818234532	27.366572424198214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	5.5
2	9.0
3	37.5
4	66.0
5	66.0
6	101.0
7	136.0
8	138.0
9	140.0
10	257.5
11	375.0
12	375.0
13	872.5
14	1370.0
15	2739.5
16	4109.0
17	3911.0
18	3713.0
19	3713.0
20	4033.0
21	4353.0
22	3207.0
23	2061.0
24	1794.5
25	1528.0
26	1528.0
27	1482.0
28	1436.0
29	1731.0
30	2026.0
31	2223.0
32	2420.0
33	2420.0
34	3214.5
35	4009.0
36	4088.5
37	4168.0
38	6614.0
39	9060.0
40	9060.0
41	12084.5
42	15109.0
43	18478.5
44	21848.0
45	35337.0
46	48826.0
47	48826.0
48	55766.0
49	62706.0
50	71174.5
51	79643.0
52	77398.0
53	75153.0
54	75153.0
55	64214.5
56	53276.0
57	47986.0
58	42696.0
59	38287.5
60	33879.0
61	33879.0
62	29880.0
63	25881.0
64	20211.5
65	14542.0
66	12274.0
67	10006.0
68	10006.0
69	8464.0
70	6922.0
71	5630.0
72	4338.0
73	3376.5
74	2415.0
75	2415.0
76	1963.0
77	1511.0
78	1231.0
79	951.0
80	709.5
81	468.0
82	468.0
83	344.5
84	221.0
85	158.5
86	96.0
87	66.0
88	36.0
89	36.0
90	19.5
91	3.0
92	2.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	541511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.613531209550576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.08936948369106	22.344126108557198
2	8.694161102995515	4.975413001228456
3	3.3791835992032273	2.9007112613580897
4	1.921165164660724	2.19885277590884
5	1.2243066190691456	1.7515867827397176
6	0.8584366004062243	1.4737741474286397
7	0.6730267355647552	1.3480370052060846
8	0.5002783686751113	1.1451784572438677
9	0.41285897030750274	1.0632017728832979
>10	3.3735503472618595	19.76196017810571
>50	0.4758400738196915	9.387475549739484
>100	0.3552681309954608	20.212877016478153
>500	0.031593717638434805	6.217359943914875
>1k	0.010961085711293709	5.219445999207588
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2382	0.4398802609734613	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2270	0.4191973939587561	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2151	0.39722184775563196	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1915	0.35364009226036036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1821	0.3362812574444471	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1774	0.32760184003649045	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1701	0.3141210427858344	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1701	0.3141210427858344	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1690	0.3120896897754616	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1647	0.30414894618945876	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1578	0.29140682276075647	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1461	0.2698006134686092	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1444	0.2666612497253057	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1375	0.2539191262966034	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1374	0.253734457841115	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1006	0.18577646622136948	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1001	0.18485312394392728	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	969	0.17894373336829725	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	962	0.17765105417987817	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	935	0.17266500588169031	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	924	0.17063365287131746	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	919	0.16971031059387529	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	869	0.16047688781945335	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	866	0.15992288245298802	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	832	0.15364415496638112	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	823	0.15198213886698514	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	820	0.15142813350051984	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	815	0.15050479122307764	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	796	0.1469960905687973	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	783	0.1445954006474476	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	773	0.14274871609256323	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	770	0.1421947107260979	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	767	0.14164070535963258	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	764	0.14108669999316725	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	756	0.13960935234925975	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	754	0.13924001543828288	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	721	0.1331459564071644	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	714	0.13185327721874532	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	690	0.1274212342870228	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	689	0.12723656583153437	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	687	0.1268672289205575	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	672	0.1240972020882309	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	651	0.12021916452297368	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	647	0.11948049070101992	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	645	0.11911115379004304	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	633	0.11689513232418178	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	617	0.11394043703636676	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	613	0.113201763214413	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	611	0.11283242630343612	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	589	0.10876972028269047	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	581	0.10729237263878295	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	567	0.10470701426194481	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	564	0.1041530088954795	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	564	0.1041530088954795	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	564	0.1041530088954795	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	549	0.10138298206315292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.8466845548843882E-4	0.0
4	0.0	0.0	0.0	1.8466845548843882E-4	0.0
5	0.0	0.0	0.0	1.8466845548843882E-4	0.0
6	0.0	0.0	0.0	1.8466845548843882E-4	0.0
7	0.0	0.0	0.0	1.8466845548843882E-4	0.0
8	3.6933691097687765E-4	0.0	0.0	1.8466845548843882E-4	0.0
9	5.540053664653165E-4	0.0	0.0	1.8466845548843882E-4	0.0
10	7.386738219537553E-4	0.0	0.0	1.8466845548843882E-4	0.0
11	7.386738219537553E-4	0.0	0.0	1.8466845548843882E-4	0.0
12	0.001108010732930633	0.0	0.0	5.540053664653165E-4	0.0
13	0.001108010732930633	0.0	0.0	5.540053664653165E-4	0.0
14	0.001108010732930633	0.0	0.0	7.386738219537553E-4	0.0
15	0.001108010732930633	0.0	0.0	9.233422774421942E-4	0.0
16	0.001108010732930633	0.0	0.0	0.0018466845548843883	0.0
17	0.0012926791884190717	0.0	0.0	0.002954695287815021	0.0
18	0.0014773476439075106	0.0	0.0	0.003324032198791899	0.0
19	0.0016620160993959495	0.0	0.0	0.0038780375652572156	0.0
20	0.0016620160993959495	0.0	0.0	0.005909390575630042	0.0
21	0.0020313530103728272	0.0	0.0	0.009233422774421941	0.0
22	0.0020313530103728272	0.0	0.0	0.012188118062236963	0.0
23	0.0020313530103728272	0.0	0.0	0.014034802617121351	0.0
24	0.0020313530103728272	0.0	0.0	0.018466845548843883	0.0
25	0.0020313530103728272	0.0	0.0	0.02012886164823983	0.0
26	0.002216021465861266	0.0	0.0	0.026038252223869875	0.0
27	0.002216021465861266	0.0	0.0	0.0537385205471357	0.0
28	0.002216021465861266	0.0	0.0	0.14625741674684356	0.0
29	0.002400689921349705	0.0	0.0	0.3130130320529038	0.0
30	0.002400689921349705	0.0	0.0	0.49897416672976175	0.0
31	0.002400689921349705	0.0	0.0	0.9944396328052431	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTAC	20	0.0018410769	37.0	25
CTTTTTA	60	1.2678356E-9	30.833332	2
CGAACTA	25	0.005493808	29.6	24
CCTAGTT	75	1.4551915E-11	29.6	2
TAGTTCC	75	1.4551915E-11	29.6	4
CTAGTTC	75	1.4551915E-11	29.6	3
GCCTAGT	75	1.4551915E-11	29.6	1
TAGTGAT	25	0.005493808	29.6	9
TTTTACG	65	2.9831426E-9	28.461538	4
GGTATCA	1455	0.0	27.209621	1
TACCTTA	75	4.620233E-10	27.133333	31
GCTTTTT	70	6.5756467E-9	26.428572	1
GAAATTC	130	0.0	25.615385	14
TAAGGTA	140	0.0	25.107143	5
TAGCGAA	135	0.0	24.666666	10
CTAAGTC	60	1.3352255E-6	24.666666	34
TCGCTAC	90	1.4188117E-10	24.666666	27
TTTTGCT	45	1.32195E-4	24.666666	2
TCGGGTA	140	0.0	23.785715	25
ACTGTGC	70	1.9190156E-7	23.785715	8
>>END_MODULE