FastQCFastQC Report
Fri 10 Feb 2017
ERR1631787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538464
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29270.5435832293338088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.5318832828192785No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA22550.41878379984548647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21610.4013267367920604No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA20280.37662684970582994No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18530.34412699827657933No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18020.334655613002912No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17200.31942711119034883No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17080.31719854994948593No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC15730.29212723598977836No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15470.2872986866345754No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15040.2793130088548167No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT14210.26389879360551494No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13870.2575845367564034No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13550.251641706780769No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10530.1955562488857194No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9820.18237059487728058No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9680.17977060676294052No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9670.17958489332620195No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9480.17605633802816903No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9470.17587062459143044No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9290.1725277827301361No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9260.17197064241992036No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8760.16268497058299164No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8670.16101354965234446No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8290.15395643905627862No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8270.15358501218280146No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8180.15191359125215428No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8040.14931360313781422No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC7970.1480136090806442No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7910.14689932846021275No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7860.14597076127651987No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7770.1442993403458727No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7620.1415136387947941No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA7600.14114221192131693No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7510.13947079099066975No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7330.13612794912937543No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG7290.1353850953824211No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA7200.13371367445177393No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA7100.13185654008438819No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7040.13074225946395673No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6700.12442800261484518No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6570.12201372793724372No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6570.12201372793724372No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT6490.1205280204433351No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA6150.11421376359422356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.1132851964105307No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5980.1110566351696678No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5970.11087092173292921No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5960.11068520829619063No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT5840.10845664705532775No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5820.10808522018185059No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5670.10529951863077198No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5640.10474237832055625No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG5600.10399952457360195No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5550.10307095738990907No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG5450.10121382302252332No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5410.10047096927556902No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5410.10047096927556902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGA200.001841067337.03
AGAACTA200.001841067337.010
AGTATAA200.001841067337.022
ATGTTAG200.001841067337.017
TATAAGT200.001841067337.024
GTTATAC200.001841067337.03
ACATTGC250.005493780629.632
ACTAATG250.005493780629.613
CCTATAG250.005493780629.62
TATAATC250.005493780629.63
GGTATCA15200.028.8453961
GAACTAA405.934356E-527.7511
GTTAGTA405.934356E-527.7519
CCGGGAC358.863246E-426.4285729
CGTTATT509.079373E-625.90000210
TTTTACG751.3702447E-824.6666664
CTTATCC551.9002247E-523.5454543
CTTTATG551.9002247E-523.5454541
CTTAAAC400.001929861523.1253
CCTACCT400.001929861523.12533
Produced by FastQC (version 0.11.5)