##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631787.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538464
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58389047364355	34.0	31.0	34.0	31.0	34.0
2	32.74227246389731	34.0	31.0	34.0	31.0	34.0
3	32.8174882629108	34.0	31.0	34.0	31.0	34.0
4	36.25463169311226	37.0	37.0	37.0	35.0	37.0
5	36.18305030605574	37.0	35.0	37.0	35.0	37.0
6	36.24945957389909	37.0	37.0	37.0	35.0	37.0
7	36.24507116538896	37.0	37.0	37.0	35.0	37.0
8	36.22866338325311	37.0	37.0	37.0	35.0	37.0
9	38.05602231532656	39.0	38.0	39.0	35.0	39.0
10	37.99179146609615	39.0	38.0	39.0	35.0	39.0
11	38.070017679919175	39.0	38.0	39.0	37.0	39.0
12	37.99585116182326	39.0	38.0	39.0	35.0	39.0
13	38.06366256611398	39.0	38.0	39.0	35.0	39.0
14	39.393998484578354	40.0	39.0	41.0	37.0	41.0
15	39.411241977179536	40.0	39.0	41.0	37.0	41.0
16	39.33301947762524	40.0	39.0	41.0	36.0	41.0
17	39.324827657930705	40.0	39.0	41.0	36.0	41.0
18	39.379507265109645	40.0	39.0	41.0	37.0	41.0
19	39.398609749212575	40.0	39.0	41.0	37.0	41.0
20	39.409030130147976	40.0	39.0	41.0	37.0	41.0
21	39.36840160159268	40.0	39.0	41.0	36.0	41.0
22	39.328239213763595	40.0	39.0	41.0	36.0	41.0
23	39.274343317287695	40.0	39.0	41.0	36.0	41.0
24	39.2714387591371	40.0	39.0	41.0	36.0	41.0
25	39.22492311463719	40.0	39.0	41.0	36.0	41.0
26	39.13410924407203	40.0	39.0	41.0	36.0	41.0
27	39.01035723836691	40.0	39.0	41.0	35.0	41.0
28	39.00094899566174	40.0	39.0	41.0	35.0	41.0
29	38.963780308432874	40.0	38.0	41.0	35.0	41.0
30	38.88759137101088	40.0	38.0	41.0	35.0	41.0
31	38.76750349141261	40.0	38.0	41.0	35.0	41.0
32	38.706860625779996	40.0	38.0	41.0	35.0	41.0
33	38.61449047661496	40.0	38.0	41.0	35.0	41.0
34	38.57671450644797	40.0	38.0	41.0	35.0	41.0
35	38.47376611992631	40.0	38.0	41.0	35.0	41.0
36	38.405280575860225	40.0	38.0	41.0	35.0	41.0
37	38.33346890414215	40.0	38.0	41.0	34.0	41.0
38	38.28664126106852	40.0	38.0	41.0	34.0	41.0
39	38.179076038509535	40.0	37.0	41.0	34.0	41.0
40	37.83979987520057	40.0	37.0	41.0	33.0	41.0
41	37.86645346764129	40.0	37.0	41.0	33.0	41.0
42	37.80676888334225	40.0	37.0	41.0	33.0	41.0
43	37.3147434183158	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	3.0
19	7.0
20	13.0
21	32.0
22	60.0
23	153.0
24	259.0
25	481.0
26	825.0
27	1355.0
28	2066.0
29	2982.0
30	4079.0
31	5588.0
32	7503.0
33	9899.0
34	14396.0
35	21417.0
36	32154.0
37	62065.0
38	129999.0
39	243124.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.39856332085339	16.628781125571997	14.009478813811139	26.96317673976348
2	20.352521245617165	20.449835086468177	33.19701967076722	26.000623997147443
3	21.08701788791823	20.568877399417605	30.58663160396981	27.75747310869436
4	14.47543382658822	17.876961133891957	35.88782165567243	31.759783383847388
5	16.18696885957093	32.66532210138468	34.29662892969632	16.85108010934807
6	29.659550127770846	35.14013935936293	17.275249598858977	17.92506091400725
7	28.101414393534203	28.758839959588755	21.492801747192015	21.64694389968503
8	26.452650502169135	30.148347893266774	21.625586854460092	21.773414750104
9	28.94696767100493	12.828712783027276	19.159683841445297	39.064635704522495
10	20.032722707553337	23.31260771379331	29.072881381113685	27.58178819753967
11	37.60418523801034	20.409349557259166	19.005913115825756	22.980552088904737
12	21.588629880549117	25.64405419860938	27.19568996256017	25.571625958281334
13	35.49893771914186	18.23928804896892	21.397716467581862	24.86405776430736
14	23.15846556130029	22.500854281808998	25.40652671302074	28.93415344386997
15	30.24491888037083	24.313788851250965	21.45621620015451	23.985076068223687
16	22.833652760444522	25.0804139181078	24.871300588340166	27.214632733107507
17	25.42138378795983	24.66497296012361	22.970709276757592	26.942933975158972
18	26.023838176739762	20.995832590479584	24.757086824745944	28.223242408034704
19	26.907276995305164	22.905895287335827	25.708311047720922	24.478516669638083
20	29.73067837404172	20.346392702204792	25.055342604148095	24.867586319605394
21	26.292751233137217	22.66483924644916	24.13828965353301	26.90411986688061
22	27.342403577583646	23.477521245617165	23.60176353479527	25.57831164200392
23	26.88981993225174	22.28431241457182	24.84604356094372	25.97982409223272
24	26.27752273132466	22.017256492541748	25.804696321388242	25.900524454745348
25	26.121523444464255	22.55396832471623	25.90256730254947	25.421940928270043
26	26.096823557378023	23.839291020383907	24.6586587032745	25.40522671896357
27	25.207441908836987	22.23324121946871	27.376017709633327	25.183299162060973
28	24.694501396565045	23.672891781066145	24.08777559874012	27.54483122362869
29	25.914267249064004	24.18917513519938	24.49040232958935	25.406155286147264
30	25.57199738515481	22.028213585309324	26.25003714268735	26.149751886848517
31	26.80253461698461	22.96959499613716	24.942985974921257	25.284884411956977
32	24.295774647887324	22.987052059190585	24.88244339454448	27.83472989837761
33	24.017204492779463	22.159698698520238	27.75840167587805	26.06469513282225
34	24.71455844773281	22.574582516194212	26.182251738277767	26.528607297795208
35	23.848576692220835	24.90361472633268	26.831505913115826	24.41630266833066
36	24.859786355262376	23.498135437095144	26.599921257502825	25.042156950139656
37	25.372541154097583	23.579106495513162	26.117437748856005	24.93091460153325
38	24.737215487014915	22.447925952338505	26.44113626909134	26.37372229155524
39	24.36801717477863	21.349802400903307	27.68838771022761	26.593792714090448
40	24.118046888928507	22.88528109585785	27.948943661971832	25.047728353241816
41	22.012985083496762	21.872214298448924	29.165366672609494	26.949433945444824
42	20.181479170380936	22.488040054674034	30.117333749331433	27.213147025613598
43	19.8861948059666	23.746062875141142	29.227023533606705	27.14071878528555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	11.5
2	12.0
3	48.0
4	84.0
5	84.0
6	126.5
7	169.0
8	200.0
9	231.0
10	347.5
11	464.0
12	464.0
13	1061.0
14	1658.0
15	3298.5
16	4939.0
17	4564.5
18	4190.0
19	4190.0
20	4706.0
21	5222.0
22	3711.5
23	2201.0
24	1925.0
25	1649.0
26	1649.0
27	1511.0
28	1373.0
29	1535.5
30	1698.0
31	2050.0
32	2402.0
33	2402.0
34	3671.0
35	4940.0
36	5410.0
37	5880.0
38	7994.0
39	10108.0
40	10108.0
41	13165.5
42	16223.0
43	20657.0
44	25091.0
45	38351.0
46	51611.0
47	51611.0
48	57398.0
49	63185.0
50	71124.5
51	79064.0
52	75294.0
53	71524.0
54	71524.0
55	61548.0
56	51572.0
57	46423.5
58	41275.0
59	36383.5
60	31492.0
61	31492.0
62	27620.5
63	23749.0
64	18383.0
65	13017.0
66	10971.0
67	8925.0
68	8925.0
69	7389.5
70	5854.0
71	4720.0
72	3586.0
73	2725.5
74	1865.0
75	1865.0
76	1539.0
77	1213.0
78	966.0
79	719.0
80	616.5
81	514.0
82	514.0
83	427.5
84	341.0
85	270.0
86	199.0
87	166.0
88	133.0
89	133.0
90	97.5
91	62.0
92	39.5
93	17.0
94	9.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	538464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.64147562345079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40870419049732	23.2414969392899
2	8.563263733109691	5.076555464043023
3	3.486989098103462	3.1007850705201725
4	1.9586087502873786	2.32224214110083
5	1.2591994047207897	1.8662264230047516
6	0.8813048055782388	1.5673904946826442
7	0.6509164973133174	1.3505887841610267
8	0.4895529902502185	1.1608858421511434
9	0.4025741482067526	1.073960262063173
>10	3.0905727991870884	18.47547552509108
>50	0.42546118037546177	8.795776168240899
>100	0.34087022404160705	19.955009729071392
>500	0.03195658350390067	6.597992959870551
>1k	0.010025594824753148	5.415614196709423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2927	0.5435832293338088	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2864	0.5318832828192785	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2255	0.41878379984548647	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2161	0.4013267367920604	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2028	0.37662684970582994	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1853	0.34412699827657933	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1802	0.334655613002912	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1720	0.31942711119034883	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1708	0.31719854994948593	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1573	0.29212723598977836	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1547	0.2872986866345754	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1504	0.2793130088548167	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1421	0.26389879360551494	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1387	0.2575845367564034	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1355	0.251641706780769	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1053	0.1955562488857194	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	982	0.18237059487728058	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	968	0.17977060676294052	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	967	0.17958489332620195	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	948	0.17605633802816903	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	947	0.17587062459143044	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	929	0.1725277827301361	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	926	0.17197064241992036	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	876	0.16268497058299164	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	867	0.16101354965234446	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	829	0.15395643905627862	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	827	0.15358501218280146	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	818	0.15191359125215428	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	804	0.14931360313781422	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	797	0.1480136090806442	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	791	0.14689932846021275	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	786	0.14597076127651987	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	777	0.1442993403458727	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	762	0.1415136387947941	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	760	0.14114221192131693	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	751	0.13947079099066975	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	733	0.13612794912937543	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	729	0.1353850953824211	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	720	0.13371367445177393	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	710	0.13185654008438819	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	704	0.13074225946395673	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	670	0.12442800261484518	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	657	0.12201372793724372	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	657	0.12201372793724372	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	649	0.1205280204433351	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	615	0.11421376359422356	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.1132851964105307	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	598	0.1110566351696678	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	597	0.11087092173292921	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	596	0.11068520829619063	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	584	0.10845664705532775	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	582	0.10808522018185059	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	567	0.10529951863077198	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	564	0.10474237832055625	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	560	0.10399952457360195	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	555	0.10307095738990907	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	545	0.10121382302252332	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	541	0.10047096927556902	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	541	0.10047096927556902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.857134367385749E-4	0.0	0.0	0.0	0.0
3	1.857134367385749E-4	0.0	0.0	0.0	0.0
4	1.857134367385749E-4	0.0	0.0	0.0	0.0
5	1.857134367385749E-4	0.0	0.0	0.0	0.0
6	1.857134367385749E-4	0.0	0.0	0.0	0.0
7	1.857134367385749E-4	0.0	0.0	0.0	0.0
8	7.428537469542997E-4	0.0	0.0	0.0	0.0
9	7.428537469542997E-4	0.0	0.0	1.857134367385749E-4	0.0
10	7.428537469542997E-4	0.0	0.0	1.857134367385749E-4	0.0
11	7.428537469542997E-4	0.0	0.0	1.857134367385749E-4	0.0
12	7.428537469542997E-4	0.0	0.0	3.714268734771498E-4	0.0
13	7.428537469542997E-4	0.0	0.0	3.714268734771498E-4	0.0
14	7.428537469542997E-4	0.0	0.0	5.571403102157248E-4	0.0
15	9.285671836928745E-4	0.0	0.0	0.0014857074939085993	0.0
16	9.285671836928745E-4	0.0	0.0	0.0024142746776014737	0.0
17	0.0011142806204314495	0.0	0.0	0.004271409044987223	0.0
18	0.0012999940571700244	0.0	0.0	0.005385689665418672	0.0
19	0.0012999940571700244	0.0	0.0	0.006685683722588697	0.0
20	0.0012999940571700244	0.0	0.0	0.008542818089974445	0.0
21	0.0012999940571700244	0.0	0.0	0.013185654008438819	0.0
22	0.0012999940571700244	0.0	0.0	0.020242764604504665	0.0
23	0.0012999940571700244	0.0	0.0	0.022842752718844714	0.0
24	0.0012999940571700244	0.0	0.0	0.028599869257740537	0.0
25	0.0012999940571700244	0.0	0.0	0.03064271706186486	0.0
26	0.0012999940571700244	0.0	0.0	0.036399833600760684	0.0
27	0.0012999940571700244	0.0	0.0	0.06351399536459262	0.0
28	0.0012999940571700244	0.0	0.0	0.1636135377666845	0.0
29	0.0014857074939085993	0.0	0.0	0.33651274737029774	0.0
30	0.0016714209306471742	0.0	0.0	0.5406118143459916	0.0
31	0.0016714209306471742	0.0	0.0	1.13768051346051	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATAGA	20	0.0018410673	37.0	3
AGAACTA	20	0.0018410673	37.0	10
AGTATAA	20	0.0018410673	37.0	22
ATGTTAG	20	0.0018410673	37.0	17
TATAAGT	20	0.0018410673	37.0	24
GTTATAC	20	0.0018410673	37.0	3
ACATTGC	25	0.0054937806	29.6	32
ACTAATG	25	0.0054937806	29.6	13
CCTATAG	25	0.0054937806	29.6	2
TATAATC	25	0.0054937806	29.6	3
GGTATCA	1520	0.0	28.845396	1
GAACTAA	40	5.934356E-5	27.75	11
GTTAGTA	40	5.934356E-5	27.75	19
CCGGGAC	35	8.863246E-4	26.428572	9
CGTTATT	50	9.079373E-6	25.900002	10
TTTTACG	75	1.3702447E-8	24.666666	4
CTTATCC	55	1.9002247E-5	23.545454	3
CTTTATG	55	1.9002247E-5	23.545454	1
CTTAAAC	40	0.0019298615	23.125	3
CCTACCT	40	0.0019298615	23.125	33
>>END_MODULE