FastQCFastQC Report
Fri 10 Feb 2017
ERR1631786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028027
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC32550.3166259251945718No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT24880.24201698982614267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.18345821656435093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16940.16478166429480937No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16930.16468439058507217No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16050.15612430412819897No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG14530.14133870024814524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14480.14085233169945927No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT14080.13696138330997143No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13980.13598864621259948No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13710.13336225604969518No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT13540.13170860298416287No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13540.13170860298416287No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG13280.12917948653099579No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT12730.12382943249545002No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12500.12159213717149453No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12290.11954938926701342No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12260.11925756813780182No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG12200.11867392587937865No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC11930.11604753571647437No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11450.11137839764908898No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC11430.11118385022961458No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11180.1087520074861847No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG11150.1084601863569731No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11120.10816836522776153No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG10890.10593106990380602No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10880.10583379619406882No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT10800.10505560651617127No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG10550.10262376377274138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.028.8403051
CTATTAG509.089867E-625.9000021
GACCGTC400.001931048823.1250027
AGACCGT652.682389E-622.7692286
TAGACTA603.7257927E-521.5833345
ACAGGCT8900.021.2022468
TTTTACG705.1010593E-621.1428574
GACAGGC8700.021.0517257
ATACGGC806.958635E-720.812529
GCGACTT450.003825537620.55555517
GGTTAGA555.1425863E-420.1818181
GACTATG759.264209E-619.7333347
GGACAGG10150.019.3201966
AGGCCAT9250.018.625
GTTTAGG1601.8189894E-1218.51
CCGTCTA500.00703438818.52
GTATCAA20200.018.52
CGTAAAA902.1517662E-618.533
ACCGTCG500.00703438818.58
TCCAGGA10750.018.0697692
Produced by FastQC (version 0.11.5)