##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631786.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028027
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60485473630556	34.0	31.0	34.0	31.0	34.0
2	32.769775502005295	34.0	31.0	34.0	31.0	34.0
3	32.853227590325936	34.0	31.0	34.0	31.0	34.0
4	36.290721936291554	37.0	37.0	37.0	35.0	37.0
5	36.21095652156996	37.0	35.0	37.0	35.0	37.0
6	36.2564319808721	37.0	37.0	37.0	35.0	37.0
7	36.239259280155096	37.0	37.0	37.0	35.0	37.0
8	36.21581631610843	37.0	37.0	37.0	35.0	37.0
9	38.05307156329552	39.0	38.0	39.0	35.0	39.0
10	37.98596048547363	39.0	38.0	39.0	35.0	39.0
11	38.066319269824625	39.0	38.0	39.0	35.0	39.0
12	38.007334437714185	39.0	38.0	39.0	35.0	39.0
13	38.05028467151155	39.0	38.0	39.0	35.0	39.0
14	39.44424611415848	40.0	39.0	41.0	37.0	41.0
15	39.44915649102602	40.0	39.0	41.0	37.0	41.0
16	39.40357500338026	40.0	39.0	41.0	37.0	41.0
17	39.363972930672055	40.0	39.0	41.0	36.0	41.0
18	39.35870555928979	40.0	39.0	41.0	36.0	41.0
19	39.37891319975059	40.0	39.0	41.0	36.0	41.0
20	39.361334867663984	40.0	39.0	41.0	36.0	41.0
21	39.33676061037308	40.0	39.0	41.0	36.0	41.0
22	39.311775858027076	40.0	39.0	41.0	36.0	41.0
23	39.25082123329446	40.0	39.0	41.0	36.0	41.0
24	39.26389676535733	40.0	39.0	41.0	36.0	41.0
25	39.203100696771585	40.0	39.0	41.0	36.0	41.0
26	39.13741078784896	40.0	39.0	41.0	36.0	41.0
27	39.05318245532462	40.0	39.0	41.0	35.0	41.0
28	39.03382401434982	40.0	39.0	41.0	35.0	41.0
29	39.013722402232624	40.0	39.0	41.0	35.0	41.0
30	38.961728631640995	40.0	38.0	41.0	35.0	41.0
31	38.89342984182322	40.0	38.0	41.0	35.0	41.0
32	38.84518986369035	40.0	38.0	41.0	35.0	41.0
33	38.789456891696425	40.0	38.0	41.0	35.0	41.0
34	38.73387469395259	40.0	38.0	41.0	35.0	41.0
35	38.64970472565409	40.0	38.0	41.0	35.0	41.0
36	38.6150947397296	40.0	38.0	41.0	35.0	41.0
37	38.57697900930618	40.0	38.0	41.0	35.0	41.0
38	38.553778256796754	40.0	38.0	41.0	35.0	41.0
39	38.48758544279479	40.0	38.0	41.0	34.0	41.0
40	38.14619849478662	40.0	37.0	41.0	34.0	41.0
41	38.211543082039675	40.0	37.0	41.0	34.0	41.0
42	38.19100762917706	40.0	37.0	41.0	34.0	41.0
43	37.72021746510549	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	5.0
19	14.0
20	27.0
21	66.0
22	123.0
23	266.0
24	478.0
25	788.0
26	1252.0
27	2095.0
28	3301.0
29	4856.0
30	6920.0
31	9688.0
32	13121.0
33	18121.0
34	27026.0
35	42692.0
36	62267.0
37	107838.0
38	237266.0
39	489813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42726406991256	15.756881871779632	12.214854279119129	28.60099977918868
2	20.38477588623645	18.237458743787858	33.76302373381244	27.614741636163252
3	21.280277658077075	19.745784886972814	28.543900111572945	30.43003734337717
4	15.646962579776602	15.038515525370444	32.95643013267161	36.358091762181346
5	17.207038336541743	32.912267868450925	32.600505628743214	17.280188166264114
6	35.67552214095544	32.57949450743998	15.058359362156832	16.68662398944775
7	31.59508456489956	27.231969588347386	19.684405176128642	21.48854067062441
8	28.29040482399781	31.05015724295179	19.286361155884038	21.373076777166357
9	28.997584693787225	12.949854429893378	17.133304864560948	40.91925601175845
10	19.865820644788514	23.59675378175865	29.141160689359324	27.39626488409351
11	38.641494824552275	19.863583349464555	18.74376840296996	22.75115342301321
12	24.58291465107434	23.1504620014844	25.93161463658056	26.33500871086071
13	33.627716003568	17.415106801669605	21.46694590706275	27.49023128769964
14	24.12543639418031	19.4701111935776	23.481289888300598	32.92316252394149
15	29.40253514742317	23.48916905878931	20.51911087938352	26.589184914404
16	25.910117146728634	23.69276293326926	21.529687449843244	28.86743247015886
17	26.468565514329878	23.96337839375814	22.160507457488958	27.407548634423023
18	26.411465846714144	21.3682130916795	23.76951189025191	28.450809171354447
19	28.115603967600073	21.94757530687424	23.106007916134498	26.83081280939119
20	28.184181932964798	21.45488396705534	23.492768186049588	26.868165913930277
21	28.772785150584568	21.778999967899676	22.09961411519347	27.348600766322285
22	28.81286191899629	23.045503668677963	21.36928310248661	26.772351309839138
23	28.118327631472713	22.622946673579587	21.9710182709209	27.287707424026802
24	28.257234488977428	21.92870420718522	22.419060977970425	27.395000325866924
25	27.466301955104296	21.814115777114804	23.387712579533417	27.33186968824749
26	28.07883450531941	22.50456456882942	23.029550780281063	26.387050145570107
27	28.10179110081739	21.767229849021476	22.999979572520953	27.130999477640177
28	26.2777144958255	22.2069070170336	23.041709993998214	28.47366849314269
29	27.581571301142873	22.465265990095588	23.135190029055657	26.817972679705882
30	26.69920148011677	21.550114928888053	24.25305950135551	27.49762408963967
31	27.03703307403405	22.2103115968744	23.32506831046266	27.42758701862889
32	25.40604478287049	22.163425668781073	23.605702963054473	28.824826585293966
33	26.36516356087924	22.249318354479016	24.436809539049072	26.948708545592677
34	26.66690660848402	22.278792288529388	24.024660830892575	27.029640272094017
35	26.6982287430194	22.98568033718959	24.454999722769927	25.86109119702109
36	26.68626407672172	22.03171706579691	23.895773165490787	27.38624569199058
37	25.59913309669882	22.06391466371992	24.63048149513583	27.706470744445426
38	26.358159853778158	20.87600812040929	25.470342705006775	27.29548932080578
39	24.731062511004087	20.612980009279912	26.64677095056842	28.009186529147577
40	24.918508949667665	20.868226223630312	27.573886678073627	26.63937814862839
41	23.187231463765055	20.276802068428164	28.545164669799526	27.990801798007254
42	22.062747379203078	21.320548973908274	28.56646761223198	28.050236034656677
43	21.762463437244353	20.5564639839226	29.049626128496627	28.631446450336423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	27.0
2	25.0
3	47.0
4	69.0
5	69.0
6	102.5
7	136.0
8	155.0
9	174.0
10	249.5
11	325.0
12	325.0
13	689.0
14	1053.0
15	2130.5
16	3208.0
17	3085.5
18	2963.0
19	2963.0
20	3332.5
21	3702.0
22	2963.0
23	2224.0
24	2333.5
25	2443.0
26	2443.0
27	2839.5
28	3236.0
29	4259.0
30	5282.0
31	6933.0
32	8584.0
33	8584.0
34	11659.5
35	14735.0
36	17206.5
37	19678.0
38	23977.0
39	28276.0
40	28276.0
41	32706.0
42	37136.0
43	43692.5
44	50249.0
45	60033.0
46	69817.0
47	69817.0
48	75826.5
49	81836.0
50	93196.5
51	104557.0
52	106879.5
53	109202.0
54	109202.0
55	103724.5
56	98247.0
57	90302.5
58	82358.0
59	75406.0
60	68454.0
61	68454.0
62	65950.5
63	63447.0
64	50888.0
65	38329.0
66	34256.5
67	30184.0
68	30184.0
69	26345.0
70	22506.0
71	21210.0
72	19914.0
73	22764.0
74	25614.0
75	25614.0
76	22059.5
77	18505.0
78	12493.0
79	6481.0
80	4278.0
81	2075.0
82	2075.0
83	1735.0
84	1395.0
85	1099.0
86	803.0
87	646.5
88	490.0
89	490.0
90	342.5
91	195.0
92	135.5
93	76.0
94	44.5
95	13.0
96	13.0
97	7.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1028027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.077015445379594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.70035297899658	33.945373545683495
2	6.947253358559744	5.568503603079282
3	2.3790492424772953	2.8603557970824354
4	1.2728676466829383	2.040509453441444
5	0.791296513659882	1.5856401299911056
6	0.5246899086531942	1.2616803343877325
7	0.42486574268616056	1.191914565229215
8	0.3278188389318411	1.051040053692624
9	0.2629672164450657	0.948504707558764
>10	1.8717850367775408	15.154381872106976
>50	0.25219638055377347	7.067638867364812
>100	0.21892820813308084	18.41626016910457
>500	0.018835164274614426	4.881867185616899
>1k	0.007093763168361278	4.026329715660702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3255	0.3166259251945718	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	2488	0.24201698982614267	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1886	0.18345821656435093	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1694	0.16478166429480937	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1693	0.16468439058507217	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1605	0.15612430412819897	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1453	0.14133870024814524	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1448	0.14085233169945927	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1408	0.13696138330997143	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1398	0.13598864621259948	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1371	0.13336225604969518	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1354	0.13170860298416287	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1354	0.13170860298416287	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1328	0.12917948653099579	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1273	0.12382943249545002	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1250	0.12159213717149453	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1229	0.11954938926701342	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1226	0.11925756813780182	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1220	0.11867392587937865	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1193	0.11604753571647437	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1145	0.11137839764908898	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	1143	0.11118385022961458	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1118	0.1087520074861847	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	1115	0.1084601863569731	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1112	0.10816836522776153	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	1089	0.10593106990380602	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1088	0.10583379619406882	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	1080	0.10505560651617127	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1055	0.10262376377274138	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.727370973719562E-5	0.0	0.0
2	0.0	0.0	9.727370973719562E-5	0.0	0.0
3	0.0	0.0	9.727370973719562E-5	0.0	0.0
4	0.0	0.0	9.727370973719562E-5	0.0	0.0
5	0.0	0.0	9.727370973719562E-5	0.0	0.0
6	0.0	0.0	9.727370973719562E-5	0.0	0.0
7	0.0	0.0	9.727370973719562E-5	0.0	0.0
8	9.727370973719562E-5	0.0	9.727370973719562E-5	0.0	0.0
9	9.727370973719562E-5	0.0	9.727370973719562E-5	0.0	0.0
10	1.9454741947439125E-4	0.0	9.727370973719562E-5	0.0	0.0
11	1.9454741947439125E-4	0.0	9.727370973719562E-5	9.727370973719562E-5	0.0
12	1.9454741947439125E-4	0.0	9.727370973719562E-5	9.727370973719562E-5	0.0
13	2.9182112921158685E-4	0.0	9.727370973719562E-5	9.727370973719562E-5	0.0
14	2.9182112921158685E-4	0.0	9.727370973719562E-5	1.9454741947439125E-4	0.0
15	2.9182112921158685E-4	0.0	9.727370973719562E-5	3.890948389487825E-4	0.0
16	2.9182112921158685E-4	0.0	9.727370973719562E-5	0.001264558226583543	0.0
17	2.9182112921158685E-4	0.0	9.727370973719562E-5	0.0016536530655323256	0.0
18	4.863685486859781E-4	0.0	9.727370973719562E-5	0.0022372953239554994	0.0
19	5.836422584231737E-4	0.0	9.727370973719562E-5	0.002626390162904282	0.0
20	5.836422584231737E-4	0.0	9.727370973719562E-5	0.0036964009700134336	0.0
21	5.836422584231737E-4	0.0	9.727370973719562E-5	0.006809159681603693	0.0
22	5.836422584231737E-4	0.0	9.727370973719562E-5	0.010602834361354322	0.0
23	5.836422584231737E-4	0.0	9.727370973719562E-5	0.01245103484636104	0.0
24	5.836422584231737E-4	0.0	9.727370973719562E-5	0.015271972428739712	0.0
25	5.836422584231737E-4	0.0	9.727370973719562E-5	0.01712017291374643	0.0
26	5.836422584231737E-4	0.0	9.727370973719562E-5	0.02237295323955499	0.0
27	6.809159681603693E-4	0.0	9.727370973719562E-5	0.05029050793413013	9.727370973719562E-5
28	6.809159681603693E-4	0.0	9.727370973719562E-5	0.1602097999371612	9.727370973719562E-5
29	7.78189677897565E-4	0.0	9.727370973719562E-5	0.32324053745670106	9.727370973719562E-5
30	7.78189677897565E-4	0.0	9.727370973719562E-5	0.5378263411369546	9.727370973719562E-5
31	7.78189677897565E-4	0.0	9.727370973719562E-5	1.1364487508596564	9.727370973719562E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1315	0.0	28.840305	1
CTATTAG	50	9.089867E-6	25.900002	1
GACCGTC	40	0.0019310488	23.125002	7
AGACCGT	65	2.682389E-6	22.769228	6
TAGACTA	60	3.7257927E-5	21.583334	5
ACAGGCT	890	0.0	21.202246	8
TTTTACG	70	5.1010593E-6	21.142857	4
GACAGGC	870	0.0	21.051725	7
ATACGGC	80	6.958635E-7	20.8125	29
GCGACTT	45	0.0038255376	20.555555	17
GGTTAGA	55	5.1425863E-4	20.181818	1
GACTATG	75	9.264209E-6	19.733334	7
GGACAGG	1015	0.0	19.320196	6
AGGCCAT	925	0.0	18.6	25
GTTTAGG	160	1.8189894E-12	18.5	1
CCGTCTA	50	0.007034388	18.5	2
GTATCAA	2020	0.0	18.5	2
CGTAAAA	90	2.1517662E-6	18.5	33
ACCGTCG	50	0.007034388	18.5	8
TCCAGGA	1075	0.0	18.069769	2
>>END_MODULE